Упоредна процена параметара аргирофилних нуклеоларних организационих региона у бенигним и малигним туморима дојке

DOI: https://doi.org/10.5937/afmnai40-35516

Sažetak


Aim: We aim to evaluate the AgNOR parameters for discrimination of benign from malignant breast tumors via a new approach the total AgNOR area/nuclear area (TAA/NA).

Material and methods: Three groups, consisting of control (n=14), benign (n=18) and malignant (n=28) were included in the study. The AgNOR staining technique was performed and both mean AgNOR number and TAA/NA ratio were evaluated.

Results: While the differences between control and patient groups were statistically significant for AgNOR number (p<0.001), it was not significant between the malignant group and the benign group for mean AgNOR number (p>0.05). For the ratio of TAA / NA, the differences between the control and benign group (p <0.001), control and malignant group (p <0.001), and malignant and benign patient groups were significant. (p <0.05).

Conclusion: We thought that the evaluation of the TAA/NA rate, when compared with the AgNOR number, is able to be a more sensitive and useful tool for distinguishing benign from the malignant breast lesions.

Reference


  1. Greenlee RT, Murray T, Bolden S, Wingo PA: Cancer statistics 2000. Cancer J Clin. 2000;50:7-33. https://pubmed.ncbi.nlm.nih.gov/10735013/

  2. Derenzini M, Ploton D: Interphase nucleolar organizer regions in cancer cells. Int Rev Exp Pathol. 1992;32:149–192. https://pubmed.ncbi.nlm.nih.gov/1713900/

  3. Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet J J: Improvement in the staining and visualisation of argyroohilic proteins of the nucleolar organising regions at the optical level. Histochem J. 1986;18:5-14 Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet J J: Improvement in the staining and visualisation of argyroohilic proteins of the nucleolar organising regions at the optical level. Histochem J. 1986;18:5-14

  4. Cucer N, Imamoglu N, Tozak H Demirtas H, Sarac F, Tatlısen A, Ozturk F: Two-dimensional agnor evaluation as a prognostic variable in urinary bladder carcinoma: A different approach via total AgNOR area/nucleus area per cell. Micron. 2007;38: 674-679. https://pubmed.ncbi.nlm.nih.gov/17011198/

  5. Lewy-Trenda I, Janczukowicz J, Wierzchniewska-Lawska A: Practical application of proliferation markers (MIB-1, PCNA, AgNOR) expression analysis for differential diagnostics of nodular thyroid lesions. Wiad lek. 2006;59:32-7 https://pubmed.ncbi.nlm.nih.gov/16646289/

  6. Derenzini M: The AgNORs. Micron. 2000;31:117-120. https://pubmed.ncbi.nlm.nih.gov/10588056/

  7. Ogura S, Abe S, Sukoh N, Kunikane H, Nakajima l, Kawakami Y, lnoue K: Correlation between nucleolar organizer regions visualized by silver staining and the growth rate of lung adenocarcinoma. Cancer. 1992;70:63–68. https://pubmed.ncbi.nlm.nih.gov/1606548/

  8. Ozdamar SO, Yıldız L, Yavuz İ Karagöz F, Kandemir B, Calıkusu A, Ulusoy AN: Meme Karsinomlarında PCNA ve AgNOR değerlerinin Mitotik İndeks, ERRP Ekspresyonu, Elastik İndeks ve Klinik Evre ile İlişkisi. Türk Patoloji Dergisi. 1995;11(2):176-179 http://www.turkjpath.org/pdf/pdf_TPD_1129.pdf

  9. Trere D, Migaldi M, Trentini GP: Higher reproducibility of morphometric analysis over the counting method for interphase AgNOR quantification. Anal Cell Pathol. 1995;8(1):57-65. https://pubmed.ncbi.nlm.nih.gov/7734412/

  10. Trere D: AgNOR staining and quantification. Micron. 2000;31:127–131. https://pubmed.ncbi.nlm.nih.gov/10588058/

  11. Eroz R, Cucer N, Unluhizarci K, Ozturk F: ‘‘Tiroid papiller karsinom ve normal tiroid hücre çekirdeklerinde AgNOR sayısının değerlendirilmesi’’, Erciyes Üniversitesi Sağlık Bilimleri Dergisi (Journal of health sciences). 2010;102-107. https://dergipark.org.tr/tr/pub/eujhs/issue/44542/552470

  12. Camargo RS, Shirata NK,di Loreto C, Garcia EA, Castelo A, Longatto Filho A:  Significance of AgNOR measurement in thyroid lesions. Anal Quant Cytol Histol. 2006;28(4):188-92. https://pubmed.ncbi.nlm.nih.gov/16927638/

  13. Ceccarelli C, Trerè D, Santini D, Taffurelli M, Chieco P, Derenzini M: AgNORs in breast tumours. Micron 2000;31:143-149. https://www.researchgate.net/publication/222965285_AgNORs_in_breast_tumours

  14. Kobyakov, D. Avdalyan, A. Lazarev, A. Lushnikova, E. Nepomnyashchikh, L: Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival. Cancer Biology & Medicine. 2014;11:264-9. https://pubmed.ncbi.nlm.nih.gov/25610712/

  15. Gundog, M. Yildiz, O. Imamoglu, N. Aslan, D. Aytekin, A, Soyuer l, Soyuer S: Prognostic Significance of Two Dimensional AgNOR Evaluation in Local Advanced Rectal Cancer Treated with Chemoradiotherapy. Asian Pacific Journal of Cancer Prevention. 2016;16:8155-8161. https://pubmed.ncbi.nlm.nih.gov/26745054/

  16. Eroz R, Yilmaz S, Cucer N: Argyrophilic nucleolar organizing region associated protein synthesis in hair root cells of humans at different developmental stages and sex. Biotech Histochem. 2013;88(5):267-271. https://pubmed.ncbi.nlm.nih.gov/23480229/

  17. Eroz R, Unluhizarci K, Cucer N, Ozturk F: The Value Of Argyrophilic Nucleolar Organising Region Protein Determinations in Non-Diagnostic Fine Needle Aspiration Samples (Due To Insufficient Cell Groups) Of Thyroid Nodules. Analytical And Quantitative Cytology And Histology. 2013;35(4):226-232. https://pubmed.ncbi.nlm.nih.gov/24341126/

  18. Gazioğlu E. Cerrahi Biyopsi Yöntemleri, Meme Kanseri Sempozyum Dizisi, 2006: 49-54 https://docplayer.biz.tr/5859816-Cerrahi-biyopsi-yontemleri-prof-dr-ertu-rul-gazio-lu.html

  19. Busch H, Smetana K: Nucleoli of tumor cells. The Nucleolus. London, Academic Press. 1970;448-471

  20. Pianese G. Teuscher R: Beitrag zur Histologie und Aetiologie der Carcinoma. Histologische und experimentelle Untersuchungen. Beitr Pathol Anat Aligem Pathol. 1896;142(1):1-193

Objavljeno
2025/12/09
Broj časopisa
Vol. 40 Br. 1 (2023): Acta facultatis medicae Naissensis
Rubrika
Originalni rad / Original article