SINTEZA I KARAKTERIZACIJA ALGINATNIH MIKROČESTICA SA ALFA-TOKOFEROLOM
Sažetak
Uvod. Tehnika mikroinkapsulacije se može koristiti za zaštitu alfa-tokoferola od degradacije u nepovoljnoj sredini, kao i za poboljšanje bioraspoloživosti i produžetak roka trajanja vitamina E. Metode. Napravljeno je ukupno četiri test formulacije kalcijum-alginatnih mikročestica sa različitim sadržajem alfa-tokoferola metodom eksternog jonotropnog geliranja. Odnos vitamin E/natrijum alginat bio je 1:1 i 1:2. Sve alginatne čestice okarakterisane su određivanjem mase, veličine čestica, procentnog sadržaja alfa-tokoferola, efikasnosti inkapsulacije i sposobnosti bubrenja. Rezultati. Nakon sušenja dobijene su čestice sfernog oblika, veličine 500 do 1000 µm, što ih svrstava u kategoriju mikročestica. Veličina i stepen bubrenja se nisu značajno menjali u 0,1 M HCl, dok su u baznim uslovima fosfatnog pufera pH 6,8 i 7,4 bili povećani. Sadržaj inkapsuliranog vitamina E bio je sličan u svim formulacijama (0,30±0,010 – 0,60±0,021mg/mL). Kapaciteti punjenja se kretao u opsegu 10±0,11% I 20,45±0,22%, dok je procentualna efikasnost inkapsulacije bila između 18,94±0,32 i 31,91±0,41.
Zaključak. Sve četiri formulacije su pokazale očekivano ponašanje u različitim medijumima, koji su simulirali gastrointestinalne tečnosti in vivo (0,1 M HCl, fosfatni pufer pH 6,8 i 7,4): gastrorezistenciju i porast veličine i sposobnosti bubrenja u intestinalnim tečnostima. Pokazano je da su optimalni uslovi za inkapsulaciju alfa-tokoferola, sa najvećim kapacitetom punjenja i stepenom inkapsulacije, postignuti korišćenjem 1% natrijum alginata, 2% kalcijum hlorida i odnosa vitamin E/polimer 1:1.
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