KOENZIM Q10 UBLAŽAVA OŠTEĆENJE JETRE IZAZVANO METOTREKSATOM KOD PACOVA
Sažetak
Glavni cilj ovog istraživanja bio je ispitivanje zaštitnih efekata koenzima Q10 na oštećenje jetre izazvano metotreksatom. Studija je obavljena na 32 pacova Vistar podeljenih u 4 grupe, pri čemu je prva grupa primala normalni fiziološki rastvor, druga koenzim Q10, treća samo metotreksat i četvrta grupa istovremeno koenzim Q10 i metotreksat. Morfološke i funkcionalne promene u tkivu jetre urađene su biohemijskom analizom seruma, histo-patološkom analizom preseka tkiva jetre i određivanjem parametara oksidativnog stresa u tkivu jetre. Primena metotreksata kod pacova izazvala je značajno povećanje koncentracija AST, ALT i g-GT i značajno smanjenje količine ukupnih proteina u serumu u poređenju sa C grupom životinja. Takođe, metotreksat je značajno povećao nivoe MDA i AOPP i smanjio aktivnost katalaze u tkivu jetre. Histopatološka analiza je pokazala izraženo oštećenje jetre sa ćelijskim poremećajem jetrenih kordona i značajnim oticanjem ćelija, vakuolnom degene-racijom i znacima inflamatornog odgovora nakon primene metotreksata. U grupi pacova koji su primali koenzim Q10 8 dana nakon primene metotreksata, značajno je smanjena povreda tkiva jetre. Blagi poremećaj normalnog radijalnog rasporeda hepatocita i samo diskretno neravnomerna raspodela sadržaja hepatičnog glikogena. U istoj grupi, biohemijska analiza je pokazala značajno smanjene koncentracije serumskih parametara oštećenja jetre, a promene parametara oksidativnog stresa su statistički značajno poboljšane u poređenju sa rezultatima u grupi koja je primala samo metotreksat. Naši rezultati su potvrdili da je koenzim Q10 zaštitni agens kod hepatotoksičnosti izazvane metotreksatom, verovatno zbog njegovih anti-oksidativnih efekata.
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