ODGOVOR POTKOŽNOG TKIVA NA DVE IMPLANTIRANE MEMBRANE NA BAZI KOLAGENA RAZLIČITOG POREKLA
Sažetak
Kao glavni strukturni protein kod sisara, kolagen ispunjava osnovne zahteve da bude odgovarajuća komponenta biomaterijala koji se koriste u tkivnom inženjerstvu. Zbog svoje biokompatibilnosti i biorazgradivosti, kolagen se može koristiti u različitim oblicima u vođenoj regeneraciji mekog i koštanog tkiva. Membrane zasnovane na kolagenu, koje se često koriste za regeneraciju mekih i tvrdih tkiva, mogu se razlikovati po svom poreklu (svinjske, goveđe i konjske), fizičko-hemijskim karakteristikama kao što su arhitektura, poroznost, sposobnost apsorpcije, kao i po proizvodnim procesima, koji mogu uticati na odgovor tkiva i konačni ishod. U ovom istraživanju ispitali smo i uporedili odgovor tkiva na dve implantirane kolagenske membrane različitog porekla: svinjskog i konjskog. Koristili smo model potkožne implantacije kod BALB/c miševa, a odgovor tkiva je analiziran tri, deset i trideset dana posle implantacije. Tkivo je analizirano histološkim i histomorfometrijskim metodama. Dobijeni rezultati su pokazali da postoje varijacije u odgovoru potkožnog tkiva, obrascima ćelijske infiltracije, kao i da postoje promene u debljini membrane i brzini resorpcije; to se može pripisati razlikama u poreklu membrane, ali i razlikama u procesu proizvodnje. Možemo zaključiti da su obe membrane pogodne za primenu u vođenoj regeneraciji tkiva.
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