• Milenko Ristić Faculty of Sciences and Mathematics, University of Priština in Kosovska Mitrovica, Serbia
  • Biljana Dekić Faculty of Sciences and Mathematics, University of Priština in Kosovska Mitrovica, Serbia
  • Niko Radulović Department of Chemistry, Faculty of Sciences and Mathematics, University of Niš, Niš, Serbia
  • Marija Aksić Faculty of Sciences and Mathematics, University of Priština in Kosovska Mitrovica, Serbia
Keywords: Synthesis, Azines, Coumarins, Spectral analysis, 1H- and 13C-NMR, Antioxidant activity


In this research, the synthesis of a new azine derivative with coumarin moiety was performed in three reaction steps, starting from 4-hydroxycoumarin. The first step in synthesis was the acetylation of 4-hydroxycoumarin to yield 3-acetyl-4-hydroxycoumarin and then the obtained 3-acetyl-4-hydroxycoumarin was reacted with hydrazine hydrate and give a corresponding hydrazone. Condensation of the hydrazone with 4-ethoxy-3-methoxybenzaldehyde afforded the target compound 1-[1-(4-hydroxy-2-oxo-2H-chromen-3-yl)-ethylidene]-2-(4-etoxy-3-methoxybenzylidene)-hydrazine in a good yield. The resulting azine derivative is fully spectrally characterized, including complete assignment of 1H- and 13C-NMR spectra, as well as 2D NMR (1H-1H COSY, NOESY, HSQC and HMBC) spectra. The antioxidant activity of corresponding hydrazone and target compound was evaluated by DPPH method where hydrazone derivative displayed a significant and target azine good antioxidant activity, with IC50 (μM) values 11.69 and 216.60, respectively.


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