VALORISATION OF SHELL OF INVASIVE CRAYFISH FROM DANUBE RIVER (FAXONIUS LIMOSUS): PROTEIN EXTRACTION AND CHARACTERIZATION
Keywords:
crayfish, shell, protein, amino acid, FTIR, DPPH
Abstract
In order to deal with invasive crayfish Faxonius Limosus impact on the native crayfish, as well as fish biodiversity in the Danube River, possible solution would be to find and adopt mechanisms for its utilizing for novel valuable products production. Apart from utilizing edible part for novel food products, shell can be also considered as a source of valuable compounds. Complex structure of shell is mainly composed of three basic compounds: chitin, protein and minerals-mainly calcium carbonate.
In this paper, shell proteins were extracted using three extraction methods. The first method was to use naturally present enzymes (proteases and lipases) in crayfish wastes and recover proteins using autolysis process. To accelerate the process, UV radiation was used. Remaining two extraction methods were alkaline extraction of proteins, where in one method alkaline extraction was applied directly to the shell and in the other method alkaline extraction followed the step of acidic demineralization of the shell. Obtained protein concentrates were analyzed for yield, crude protein content, DPPH radical scavenging ability, amino acidic content and structure.
Results have shown that similar percent of protein content was obtained by all three methods: 67-68%, but extraction yield was considerably different. Alkaline deproteinization with or without the step of demineralization resulted in 10 % yield, while UV radiation accelerated autolysis resulted in only 3,41 % yield. Although proteins extraction without using exogenous enzymes or chemicals is very interesting approach, drawback of this approach is low process yield. FTIR spectroscopy revealed secondary structure that was similar in all three concentrates, according to the peak deconvolution, whit autolitic concentrate differing in a lesser extent, having slightly higher share of b sheet structures. DPPH assay revealed high antioxidant activity of the concentrates (72-88 %), probably originating from active peptides derived from proteins and residues of carotenoides led by astaxanthin.
References
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Pattanaik, S. S., Sawant, P. B., Xavier, K. A. M., Dube, K., Srivastava, P. P., Dhanabalan, V., & Chadha, N. K. (2020). Characterization of carotenoprotein from different shrimp shell waste for possible use as supplementary nutritive feed ingredient in animal diets. Aquaculture, 515(October 2019), 734594. https://doi.org/10.1016/j.aquaculture.2019.734594
Rhazi, M., Desbrières, J., Tolaimate, A., Alagui, A., & Vottero, P. (2000). Investigation of different natural sources of chitin: Influence of the source and deacetylation process on the physicochemical characteristics of chitosan. Polymer International, 49(4), 337–344. https://doi.org/10.1002/(SICI)1097-0126(200004)49:4<337::AID-PI375>3.0.CO;2-B
Ribeiro, A. G. O., Viana, M. L. P., Hattori, G. Y., Constantino, V. R. L., & Perotti, G. F. (2018). Extraction and characterization of biopolymers from exoskeleton residues of the amazon crab Dilocarcinus pagei. Revista Brasileira de Ciências Ambientais (Online), 50, 97–111. https://doi.org/10.5327/Z2176-947820180398
Roljić, R. L., Nikolić, V. P., Đikanović, V., Zorić, K. S., Urošević, A. M., & Marković, V. M. (2024). Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia. Archives of Biological Sciences, 76(1), 91–101. https://doi.org/10.2298/ABS231212005R
Satmari, A., Miok, K., Ion, M. C., Zaharia, C., Schrimpf, A., & Pârvulescu, L. (2023). Headwater refuges: Flow protects Austropotamobius crayfish from Faxonius limosus invasion. NeoBiota, 89, 71–94. https://doi.org/10.3897/NEOBIOTA.89.110085
Šimat, V., Rathod, N. B., Čagalj, M., Hamed, I., & Mekinić, I. G. (2022). Astaxanthin from Crustaceans and Their Byproducts: A Bioactive Metabolite Candidate for Therapeutic Application. Marine Drugs, 20 (3), 206. https://doi.org/10.3390/md20030206
Śmietana, N., Panicz, R., Sobczak, M., Nędzarek, A., & Śmietana, P. (2020). Variability of elements and nutritional value of spiny-cheek crayfish (Faxonius limosus, Rafinesque, 1817): Variability of elements and nutritional value of F. limosus. Journal of Food Composition and Analysis, 94 (December), 103656. https://doi.org/10.1016/j.jfca.2020.103656
Śmietana, N., Panicz, R., Sobczak, M., Śmietana, P., & Nędzarek, A. (2021). Spiny-cheek crayfish, faxonius limosus (Rafinesque, 1817), as an alternative food source. Animals, 11(1), 1–21. https://doi.org/10.3390/ani11010059
Spackman, D. H., Stein, W. H., & Moore, S. (1958). Automatic Recording Apparatus for Use in the Chromatography of Amino Acids. Analytical Chemistry, 30(7), 1190–1206. https://doi.org/10.1021/ac60139a006
Stirn, A. (2012). The Formula for Lobster Shell. https://www.mpie.de/3526359/2012_the_formula_for_lobster_shell.pdf
Sukumaran, S. (2022). Protein secondary structure elucidation using FTIR spectroscopy Application note. Thermo Fischer Scientific, 1–4. www.btools.com
Šuput, D., Pezo, L., Rakita, S., Spasevski, N., Tomičić, R., Hromiš, N., & Popović, S. (2024). Camelina sativa Oilseed Cake as a Potential Source of Biopolymer Films: A Chemometric Approach to Synthesis, Characterization, and Optimization. Coatings, 14(1), 95. https://doi.org/10.3390/coatings14010095
Suzuki, M., Sugisaka-Nobayashi, A., Kogure, T., & Nagasawa, H. (2013). Structural and functional analyses of a strong chitin-binding protein-1 (SCBP-1) from the exoskeleton of the crayfish Procambarus clarkii. Bioscience, Biotechnology and Biochemistry, 77(2), 361–368. https://doi.org/10.1271/bbb.120787
Tomičić, Z., Pezo, L., Spasevski, N., Lazarević, J., Čabarkapa, I., & Tomičić, R. (2022). Diversity of amino acids composition in cereals. Food and Feed Research, 1(49), 11–22. https://doi.org/10.5937/ffr0-34322
Trevino, S. R., Scholtz, J. M., & Pace, C. N. (2007). Amino Acid Contribution to Protein Solubility: Asp, Glu, and Ser Contribute more Favorably than the other Hydrophilic Amino Acids in RNase Sa. Journal of Molecular Biology, 366(2). https://doi.org/10.1016/j.jmb.2006.10.026
Wang, C., Shi, G., Que, F., Xia, Y., Li, X., Yang, H., Shi, L., Wu, W., Ding, A., Li, X., Qiao, Y., Liao, L., Kang, J., Wang, L., Wang, L., & Xiong, G. (2022). Effect of microstructure and chemical proximate composition on mechanical properties of Procambarus clarkii shell. Lwt, 165(February), 113731. https://doi.org/10.1016/j.lwt.2022.113731
Zhou, X., Dai, Q., Huang, X., & Qin, Z. (2021). Preparation and characterizations of antibacterial-antioxidant film from soy protein isolate incorporated with mangosteen peel extract. E-Polymers, 21(1). https://doi.org/10.1515/epoly-2021-0058
Zorić, K. S., Atanacković, A. D., Ilić, M. D., Csányi, B., & Paunović, M. M. (2020). The spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) (Decapoda: Cambaridae) invades new areas in Serbian inland waters. Acta Zoologica Bulgarica, 72(4), 623–627.
Ahmadkelayeh, S., & Hawboldt, K. (2020). Extraction of lipids and astaxanthin from crustacean by-products: A review on supercritical CO2 extraction. Trends in Food Science and Technology, 103(July), 94–108. https://doi.org/10.1016/j.tifs.2020.07.016
Ayodeji Ahmed, A. (2022). Mineral and amino profile of crab (Sudanonaonautes aubryi). Food Chemistry Advances, 1(July), 100070. https://doi.org/10.1016/j.focha.2022.100070
Bradley, M. (2007). Curve Fitting in Raman and IR Spectroscopy: Basic Theory of Line Shapes and Applications. Application Note: 50733Thermo Fisher Scientific, Madison, WI, USA, https://assets.thermofisher.com/TFS-Assets/CAD/Application-Notes/AN50733_E.pdf
KeyCao, W., Tan, C., Zhan, X., Li, H., & Zhang, C. (2014). Ultraviolet irradiation and gradient temperature assisted autolysis for protein recovery from shrimp head waste. Food Chemistry, 164, 136–141. https://doi.org/10.1016/j.foodchem.2014.05.042
Dey, S. S., & Dora, K. C. (2014). Antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (Penaeus monodon and Penaeus indicus). Journal of Food Science and Technology, 51(3), 449-457. https://doi.org/10.1007/s13197-011-0512-z
Dragojlović, D. M., Popović, L. M., Čakarević, J. C., Spasevski, N. J., Rakita, S. M., Čolović, D. S., & Đuragić, O. M. (2021). Determination of protein oxidation in aquaculture feed. Food and Feed Research, 48(2), 175-184. https://doi.org/10.5937/ffr48-34712
El-Sherif, S. A., Abou-Taleb, M., Ibrahim, S. M., Talab, A. S., & El-Ghafour, S. A. (2021). Nutritional composition and amino acid profile of the crayfish byproduct meal. Egyptian Journal of Aquatic Biology and Fisheries, 25(4), 909–915. https://doi.org/10.21608/ejabf.2021.195951
Endo, H., Takagi, Y., Ozaki, N., Kogure, T., & Watanabe, T. (2004). A crustacean Ca2+-binding protein with a glutamate-rich sequence promotes CaCO3 crystallization. Biochemical Journal, 384(1), 159–167. https://doi.org/10.1042/BJ20041052
Feng, P., Gao, M., Burgher, A., Zhou, T. H., & Pramuk, K. (2016). A nine-country study of the protein content and amino acid composition of mature human milk. Food & nutrition research, 60, 31042. https://doi.org/10.3402/fnr.v60.31042
Lazarević, J., Čabarkapa, I., Rakita, S., Banjac, M., Tomičić, Z., Škrobot, D., Radivojević, G., Kalenjuk Pivarski, B., & Tešanović, D. (2022). Invasive Crayfish Faxonius limosus: Meat Safety, Nutritional Quality and Sensory Profile. International Journal of Environmental Research and Public Health, 19(24), 16819. https://doi.org/10.3390/ijerph192416819
Li, C., Pei, J., Zhu, S., Song, Y., Xiong, X., & Xue, F. (2020). Development of chitosan/peptide films: Physical, antibacterial and antioxidant properties. Coatings, 10(12), 1193. https://doi.org/10.3390/coatings10121193
Makkey, A., Ibrahim Mahmoud, M., Ibrahim, S., Desouky, M., Abouzied, A., & Mohamed, S. (2023). Population dynamic parameters, economic evaluation of Crayfish (Procambarus clarkii) and value added of its shells from River Nile, Egypt. Egyptian Journal for Aquaculture, 12(3), 1–25. https://doi.org/10.21608/eja.2023.181764.1087
Mugisha, J., Asekova, S., Kulkarni, K. P., Park, C. W., & Lee, J. (2016). Evaluation of crude protein, crude oil, total flavonoid, total polyphenol content and DPPH activity in the sprouts from a high oleic acid soybean cultivar. Korean Journal of Agricultural Science, 43(5), 723–733. https://doi.org/10.7744/kjoas.20160075
Nadarajah, K. (2005). Development and characterization of antimicrobial edible filmsfrom crawfish chitosan (PhD thesis).[ Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College. Baton Rouge, LA. https://repository.lsu.edu/cgi/viewcontent.cgi?referer=&httpsredir=1&article=2629&context=gradschool_dissertations
Pattanaik, S. S., Sawant, P. B., Xavier, K. A. M., Dube, K., Srivastava, P. P., Dhanabalan, V., & Chadha, N. K. (2020). Characterization of carotenoprotein from different shrimp shell waste for possible use as supplementary nutritive feed ingredient in animal diets. Aquaculture, 515(October 2019), 734594. https://doi.org/10.1016/j.aquaculture.2019.734594
Rhazi, M., Desbrières, J., Tolaimate, A., Alagui, A., & Vottero, P. (2000). Investigation of different natural sources of chitin: Influence of the source and deacetylation process on the physicochemical characteristics of chitosan. Polymer International, 49(4), 337–344. https://doi.org/10.1002/(SICI)1097-0126(200004)49:4<337::AID-PI375>3.0.CO;2-B
Ribeiro, A. G. O., Viana, M. L. P., Hattori, G. Y., Constantino, V. R. L., & Perotti, G. F. (2018). Extraction and characterization of biopolymers from exoskeleton residues of the amazon crab Dilocarcinus pagei. Revista Brasileira de Ciências Ambientais (Online), 50, 97–111. https://doi.org/10.5327/Z2176-947820180398
Roljić, R. L., Nikolić, V. P., Đikanović, V., Zorić, K. S., Urošević, A. M., & Marković, V. M. (2024). Morphometric characteristics of spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) from the Danube River on the territory of Serbia. Archives of Biological Sciences, 76(1), 91–101. https://doi.org/10.2298/ABS231212005R
Satmari, A., Miok, K., Ion, M. C., Zaharia, C., Schrimpf, A., & Pârvulescu, L. (2023). Headwater refuges: Flow protects Austropotamobius crayfish from Faxonius limosus invasion. NeoBiota, 89, 71–94. https://doi.org/10.3897/NEOBIOTA.89.110085
Šimat, V., Rathod, N. B., Čagalj, M., Hamed, I., & Mekinić, I. G. (2022). Astaxanthin from Crustaceans and Their Byproducts: A Bioactive Metabolite Candidate for Therapeutic Application. Marine Drugs, 20 (3), 206. https://doi.org/10.3390/md20030206
Śmietana, N., Panicz, R., Sobczak, M., Nędzarek, A., & Śmietana, P. (2020). Variability of elements and nutritional value of spiny-cheek crayfish (Faxonius limosus, Rafinesque, 1817): Variability of elements and nutritional value of F. limosus. Journal of Food Composition and Analysis, 94 (December), 103656. https://doi.org/10.1016/j.jfca.2020.103656
Śmietana, N., Panicz, R., Sobczak, M., Śmietana, P., & Nędzarek, A. (2021). Spiny-cheek crayfish, faxonius limosus (Rafinesque, 1817), as an alternative food source. Animals, 11(1), 1–21. https://doi.org/10.3390/ani11010059
Spackman, D. H., Stein, W. H., & Moore, S. (1958). Automatic Recording Apparatus for Use in the Chromatography of Amino Acids. Analytical Chemistry, 30(7), 1190–1206. https://doi.org/10.1021/ac60139a006
Stirn, A. (2012). The Formula for Lobster Shell. https://www.mpie.de/3526359/2012_the_formula_for_lobster_shell.pdf
Sukumaran, S. (2022). Protein secondary structure elucidation using FTIR spectroscopy Application note. Thermo Fischer Scientific, 1–4. www.btools.com
Šuput, D., Pezo, L., Rakita, S., Spasevski, N., Tomičić, R., Hromiš, N., & Popović, S. (2024). Camelina sativa Oilseed Cake as a Potential Source of Biopolymer Films: A Chemometric Approach to Synthesis, Characterization, and Optimization. Coatings, 14(1), 95. https://doi.org/10.3390/coatings14010095
Suzuki, M., Sugisaka-Nobayashi, A., Kogure, T., & Nagasawa, H. (2013). Structural and functional analyses of a strong chitin-binding protein-1 (SCBP-1) from the exoskeleton of the crayfish Procambarus clarkii. Bioscience, Biotechnology and Biochemistry, 77(2), 361–368. https://doi.org/10.1271/bbb.120787
Tomičić, Z., Pezo, L., Spasevski, N., Lazarević, J., Čabarkapa, I., & Tomičić, R. (2022). Diversity of amino acids composition in cereals. Food and Feed Research, 1(49), 11–22. https://doi.org/10.5937/ffr0-34322
Trevino, S. R., Scholtz, J. M., & Pace, C. N. (2007). Amino Acid Contribution to Protein Solubility: Asp, Glu, and Ser Contribute more Favorably than the other Hydrophilic Amino Acids in RNase Sa. Journal of Molecular Biology, 366(2). https://doi.org/10.1016/j.jmb.2006.10.026
Wang, C., Shi, G., Que, F., Xia, Y., Li, X., Yang, H., Shi, L., Wu, W., Ding, A., Li, X., Qiao, Y., Liao, L., Kang, J., Wang, L., Wang, L., & Xiong, G. (2022). Effect of microstructure and chemical proximate composition on mechanical properties of Procambarus clarkii shell. Lwt, 165(February), 113731. https://doi.org/10.1016/j.lwt.2022.113731
Zhou, X., Dai, Q., Huang, X., & Qin, Z. (2021). Preparation and characterizations of antibacterial-antioxidant film from soy protein isolate incorporated with mangosteen peel extract. E-Polymers, 21(1). https://doi.org/10.1515/epoly-2021-0058
Zorić, K. S., Atanacković, A. D., Ilić, M. D., Csányi, B., & Paunović, M. M. (2020). The spiny-cheek crayfish Faxonius limosus (Rafinesque, 1817) (Decapoda: Cambaridae) invades new areas in Serbian inland waters. Acta Zoologica Bulgarica, 72(4), 623–627.
Published
2024/10/23
Section
Original research paper