Journal of Medical Biochemistry

Analiza faktora koji utiču na težinu nakon barijatrijske-metabolske operacije u bolesnika sindromu obesitosti hipoventilacije na osnovu gastrointestinalnih peptida hormona

2025-11-02T16:18:06+01:00

Background: Obesity is becoming increasingly prevalent in modern society, leading to a rise in the incidence of obesity hypoventilation syndrome (OHS). This study analyzes the factors influencing weight regain in OHS patients following laparoscopic sleeve gastrectomy (LSG), based on gastrointestinal peptide hormones.

Methods: A total of 134 OHS patients who underwent LSG at our hospital between January 2023 and January 2024 were enrolled. The alterations in gastrointestinal peptide hormones, including insulin (INS), leptin (Lep), glucagon-like peptide-1 (GLP-1), and ghrelin (GHR), before and after surgery were measured. Subsequently, a 6-month follow-up was conducted. Patients with weight regain were identified, and the predictive value of gastrointestinal peptide hormones for weight regain was analyzed. Logistic regression was then employed to analyze the related factors affecting weight regain.

Results: Following the surgical procedure, a significant increase was observed in the levels of INS, Lep, and GLP, accompanied by a notable decrease in GHR levels among the patients (P < 0.05). During the follow-up period, 32 patients experienced weight regain. The receiver operating characteristic (ROC) curve analysis demonstrated that gastrointestinal peptide hormones exhibited outstanding predictive capabilities for postoperative weight regain. Moreover, through statistical analysis, it was determined that unfavorable dietary habits, lack of regular exercise, trace element deficiencies, and negative emotional states were independent risk factors influencing weight regain following LSG (P < 0.05).

Conclusion: There is a close correlation between gastrointestinal peptide hormones and post-LSG weight alterations in patients with OHS.

Vrste ustava tradicionalne kineske medicine i apolipoprotein B u hiperurikemiji: Udruženja sa kardiovaskularnim rizikom Tradicionalna kineska medicina Ustav Tipovi i Apolipoprotein B u hiperurikemiji: Udruženja sa kardiovaskularnim rizikom

2025-11-02T15:27:04+01:00

Background: To explore the correlation between different traditional Chinese medicine (TCM) constitution types and apolipoprotein B (ApoB) in patients with hyperuricemia (HUA), and to investigate the relationships between TCM constitutions, uric acid levels, and various cardiovascular risk factors.

Methods: A cross-sectional study involving 683 patients diagnosed with HUA was conducted. Patients' TCM constitutions were classified using the standardized "Classification and Determination of TCM Constitution" questionnaire. Serum uric acid (UA), lipid profiles, ApoB, and homocysteine (Hcy) levels were measured.

Results: Among 683 HUA patients, phlegm-dampness (22.99%) and damp-heat constitution (20.06%) were the most common TCM constitution types. UA, ApoB, and Hcy levels in patients with phlegm-damp constitution were significantly higher than those in other constitutions (P<0.05). UA levels were negatively correlated with HDL-C (r=-0.472, P=0.027) and positively correlated with ApoB (r=0.618, P=0.012), and Hcy (r=0.492, P=0.018). Male gender, BMI, ApoB, Hcy, and phlegm-dampness constitution are independent factors associated with UA levels. Patients with high ApoB levels had a significantly higher incidence of cardiovascular diseases compared to those with low ApoB levels (male: 42.73% vs 22.81%, P<0.001; female: 37.82% vs 25.21%, P=0.036). Logistic regression analysis revealed that phlegm-dampness constitution (OR=2.18, 95% CI: 1.42-3.35, P<0.001) and damp-heat constitution (OR=1.86, 95% CI: 1.21-2.87, P=0.005) were independently associated with increased risk of cardiovascular diseases.

Conclusion: Phlegm-damp and damp-heat constitutions are the most common TCM constitution types in HUA patients and are associated with higher levels of UA, ApoB, and Hcy. These constitution types are independently associated with increased cardiovascular risk.

ULOGA MIKRO-RNA-346 U PROGRESIJI RAKA PROSTATE: KLINIČKI ZNAČAJ I POTENCIJAL KOJI IMA KAO BIOMARKER

2025-11-02T16:21:36+01:00

Objective: The aim of this study was to analyze the expression changes and clinical significance of microRNA-346 (MiRNA-346) in PCa genesis, development and hormone resistance transformation.

Method: Data of the middle-aged and elderly male patients who were treated in Tengzhou Central People’s Hospital from January 2017 to December 2023 were collected. The tissues and corresponding preoperative serum of 128 patients with newly diagnosed PCa and 130 patients with benign prostatic hyperplasia (BPH), 120 patients with hormone-sensitive PCa (HSPC), 116 patients with castration-resistant PCa (CRPC), and 150 healthy males of the same age who underwent physical examination in the physical examination center of our hospital were collected. Quantitative real-time polymerase chain reaction was used to detect the relative expression of miRNA-346 in the tissues and serum of the subjects. GraphPad Prism statistical analysis software was utilized to analyze the expression changes of miRNA-346 in the occurrence and progression of PCa and its correlation with clinical and pathological features.

Result: The expression of miRNA-346 was significantly lower in PCa patients than in BPH patients and healthy population (P<0.001), while there was no difference between BPH and healthy population (P=0.516). Tissue and serum expression were positively correlated (P<0.001). miRNA-346 was negatively correlated with tPSA, PHI, clinical stage, and Gleason score in PCa tissue/serum (P<0.001). Serum miRNA-346 in CRPC patients was negatively correlated with time to transformation (r=0.6575) and survival (r=0.5699) (P<0.001). Serum miRNA-346 was positively correlated with tPSA and ALP in HSPC and CRPC patients (P<0.001).

Conclusion: Serum miRNA-346 expression in the process of hormone resistance conversion in PCa is gradually increasing, making it a potential biomarker for monitoring PCa progression.

Neinvazivni prenatalni testovi za skrining hromozomskih abnormalnosti u in vitro oplodnji starijih trudnica u severozapadnoj Kini

2025-11-02T15:27:05+01:00

Background: The study aimed to explore the value of using non-invasive prenatal tests (NIPT) in the second trimester of pregnancy for chromosomal abnormality screening in vitro fertilization in elderly pregnant women and to analyze the reasons for inconsistent screening results in northwest China.

Methods: A total of 47,286 pregnant women aged 19-51 who underwent prenatal examinations were collected. NIPT detection found that the positive rate of pregnant women aged >35 with spontaneous pregnancy was 0.78%, and the positive rate of in vitro fertilization pregnancy was 0.82%. Then, the detection accuracy of NIPT for pregnant women aged >35 with in vitro fertilization was further analyzed.

Results: NIPT's sensitivity, specificity, and positive predictive value in detecting common chromosomal aneuploidies (T21, T18, and T13) in pregnant women aged >35 who received in vitro fertilization were 99.72%, 99.78%, and 66.45%, respectively. The mean gestational age, pregnancy number, AFP, and free β-HCG significantly differed between the positive and false positive groups (P < 0.05). Logistic analysis showed that the mean gestational age and AFP were independent risk factors for the failure of NIPT diagnosis (P < 0.05).

Conclusions: NIPT has a particular detection performance for common chromosomal aneuploidies in vitro fertilization in pregnant women. However, factors affecting detection accuracies must be considered when using it.

Razvoj i validacija modela nomograma za predviđanje koronarne bolesti srca kod pacijenata sa hrkanjem sa hipertenzijom i poremećajima metabolizma bez glukoze

2025-11-02T15:27:05+01:00

Background: We developed and validated a nomogram to predict the risk of coronary heart disease in hypertensive patients who snore, excluding those with glucose metabolism disorders.

Methods: Records from 2105 snoring patients with hypertension and non-glucose metabolism disorders. A random grouping technique was utilized to split the data into validation and derivation datasets (split ratio = 0.7: 0.3). Least absolute shrinkage and selection operator regression was applied to select predictors and constructed a nomogram model based on multivariate Cox regression analysis. The discrimination and consistency of the nomogram were evaluated using the area under the receiver operating characteristic curve (AUC), calibration plots, and decision curve analysis (DCA) to assess its performance. We found age, male, waist-to-height ratio (WHtR), low and high-density lipoprotein cholesterol (LDL-C and HDL-C), and apnea index (AI) identified as predictors to generate this nomogram model.

Results: The C-index at 84 months was 0.703 (95% confidence interval 0.653–0.754) in the derivation set and 0.645 (95% confidence interval 0.562–0.728) in the validation set. The nomogram demonstrated good performance in the calibration curve and DCA.

Conclusions: So, our study proposed an effective nomogram model with potential application value for individualized prediction of coronary heart disease outcomes in snoring individuals with hypertension, excluding glucose metabolism disorders. And “AI” was proposed as a novel predictor.

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2025-11-02T16:25:41+01:00

Promjene proteina hranjivih hranjivaca, upalnih faktora i imunoglobulina u cerebralnom krvarenju

2025-11-02T16:26:39+01:00

Objective: Enteral nutrition support (ENS) is one of the necessary measures after surgical treatment of modern cerebral hemorrhage (CH), and this study will analyze the changes in nutrient proteins, inflammatory factors, and immunoglobulins before and after ENS in patients with CH, which will serve as a reference for future clinics when performing ENS.

Methods: 160 ICH patients (76 in the intermittent group and 84 in the continuous group) were included, and changes in indicators before and after EN intervention were retrospectively analyzed in both groups. Including nutrient proteins (albumin ALB, transferrin TRF, prealbumin PAB), inflammatory factors (IL-1β, IL-6, TNF-α), immunoglobulins (IgA, IgG, IgM) and gastrointestinal tolerance.

Results: There was no difference in adverse reactions between the two groups during ENS (P>0.05).After ENS, ALB, TRF, PAB, IgA, IgG, and IgM were significantly increased in both groups, while IL-1β, IL-6, and TNF-α were decreased (P<0.05). After ENS, there was no difference in nutrient proteins between the two groups (P>0.05), but inflammatory factors were lower in the intermittent group than in the continuous group, while immunoglobulins were higher than in the intermittent group (P<0.05).

Conclusion: ENS constitutes the core mechanism of neuroprotection through a multilayered linkage of “metabolic substrate supply - intestinal barrier repair - immune phenotype remodeling - inflammatory signaling inhibition”.

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2025-11-02T16:27:36+01:00

ACCEPTANCE AND COMMITMENT THERAPY MODULATES IMMUNEINFLAMMATORY RESPONSES AND NEUROTROPHIC FACTORS HOMEOSTASIS IN ELDERLY STROKE PATIENTS: A RANDOMIZED CONTROLLED TRIAL

2025-11-02T16:28:30+01:00

Objective: This study examined the regulatory effects of Acceptance and Commitment Therapy (ACT) on T lymphocyte subsets, serum inflammatory cytokines, neurotransmitters, and oxidative stress markers in elderly cerebral stroke (CS) patients, providing insights into the multi-dimensional pathophysiological interactions and potential intervention strategies for chronic stroke recovery.

Methods: In this randomized controlled trial, 120 elderly stroke patients were allocated to either an ACT group (ACT intervention; n=60) or a routine group (conventional treatment; n=60). Comprehensive assessments were performed to quantify: (1) peripheral T lymphocyte distribution (CD3+, CD4+, CD8+ subsets, and CD4+/CD8+ ratio), (2) serum inflammatory cytokines (IL-1β, IL-6, IL-10, and TNF-α), (3) neurotransmitters (5-HT, NE, BDNF, and IGF-1), and (4) oxidative stress markers (SOD, MDA, CAT, and NO) using flow cytometry, HPLC-ECD, and ELISA. Statistical analyses were conducted with SPSS 22.0.

Results: Following treatment, CS patients exhibited reduced CD3+ and CD4+ T-cell levels along with a decreased CD4+/CD8+ ratio, while CD8+ T-cell proportions were elevated (P<0.05). Proinflammatory cytokine levels (IL-1β, IL-6, and TNF-α) were significantly suppressed, whereas anti-inflammatory IL-10 expression increased (P<0.05). Notably, ACT demonstrated superior efficacy in restoring immune balance and attenuating inflammation compared to conventional intervention (P<0.05). Furthermore, neurotransmitter levels were elevated, and oxidative stress markers were ameliorated in CS after treatment (P<0.05), suggesting that ACT enhances neurotrophic activity and mitigates oxidative injury.

Conclusion: ACT likely confers neuroprotection through multi-target mechanisms, including modulation of T-cell subset homeostasis, upregulation of neurotrophic factors, and suppression of oxidative stress. These findings provide a scientific foundation for integrating ACT into a psychobiological rehabilitation framework for elderly CS patients.

Удружење за миР-223-3п и миР-223-5п експресија гена са нивоима ИгЕ, витамина Д и Мг+2 код педијатријских пацијената са астмом

2025-11-02T16:29:41+01:00

Позадина: Астма, глобална незаразна болест, значајно утиче на јавно здравље. Тешки случајеви узрокују високе стопе морбидитета и морталитета, при чему стопа астме код деце у Ираку достиже 20%. Студија је истраживала нивое експресије миР-223-3п и миР-223-5п гена повезаних са педијатријском астмом и утицај ИгЕ, витамина Д и Мг+2 на тежину астме.

Методе: Студија на 140 деце узраста 1-10 година са алергијском астмом спроведена је у Багдаду од новембра 2023. до фебруара 2024. Пацијенти су подељени у три групе: млађи од 1 године, они од 1-5 година и старији од 5 година. Серум ИгЕ, Нивои витамина Д3 и Мг+2 су одређени коришћењем комплета за тестирање имуноглобулина Е.

Резултати: Резултати су указали на значајно повећање нивоа ИгЕ код пацијената (353,812 ± 25,679 нг/мл) у поређењу са контролном групом (25,320 ± 2,581 нг/мл) (п-вредност < 0,01, ниво Вит-Д у серуму је открио значајно смањење у групи пацијената (16,907 ± 0,512 нг/мЛ), док је у контролној групи нивои достигли (32,746 ± 1,629 нг/мЛ), ниво Мг⁺² у серуму смањен у групама пацијената (2,168 ± 0,030 нг/дЛ) у поређењу са контролној групи (2,316 ± 0,028 нг/дЛ) при п-вредности < 0,01, такође, ова студија показује да се повећање експресије гена у генима миР-223-3п и миР-223-5п сматра факторима ризика за алергијску астму. Закључак: Студија је открила да пацијенти са астмом имају висок ниво ИгЕ, низак ниво витамина Д3 и магнезијума и високу експресију гена у генима миР-223-3п и миР-223-5п, факторима ризика за алергијску астму.

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2025-11-18T10:12:34+01:00

The

2025-12-05T17:13:35+01:00

Background: This is largely because depression is a common psychiatric condition affecting more than 280 million people worldwide and is increasingly linked to an increased risk of thrombotic events, including deep vein thrombosis (DVT) and pulmonary embolism (PE). For example, underlying pathophysiological mechanisms remain inadequatly understood and thus further research on the association between depression, systemic inflammation, platelet activation and coagulation abnormalities is warranted. The objective of this study is to identify biological pathways linking thombosis and depression by examination of inflammatory and coagulation biomarkers, platelet activation, and risk of thrombosis independent predictors. In addition, the study investigates the possibility of nursing interventions in reducing thrombotic complications among depressed individuals.

Methods: A case control study was performed with 500 subjects (250 who had major depressive disorder and 250 healthy control subjects) were included. Enzyme-linked immunosorbent assays (ELISA) and quantitative polymerase chain reaction (qPCR) were employed to quantify key inflammatory (IL-6, TNF-, CRP) and coagulation (D-dimer, fibrinogen) biomarkers. An assessment of platelet activation (CD62P, PAC1 binding, GPIIbIIIa activation) was performed by flow cytometry. The study was carried out by a longitudinal follow-up over 12 months and by multivariate regression models to identify independent risk predictors.

Results: Thrombophilic and inflammatory parameters were significantly higher in depressed patients as compared to controls (p 0.001). The system was markedly hypercoagulable, as platelet activation markers were greatly upregulated. Through multivariate regression analysis, we determined that thrombosis risk was independent of severity level of depression (OR = 2.10, p 0.001), IL-6 levels (OR = 1.92, p 0.001), and platelet activation (OR = 2.50, p 0.001).

Conclusion: The results indicate that depression is an independent risk factor for thrombosis, through systemic inflammation and platelet hyperactivity. These results reinforce the value of licking psychiatric screening into thrombosis risk assessment and suggest the possible benefits of targeted anti-inflammatory or antiplatelet interventions in the psychiatric population at high risk of thrombosis.

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2025-11-02T16:32:44+01:00

MARKERI UPALE I IMUNOLOŠKE KOMPETENCIJE: PROCENA U RANOJ POSTOPERATIVNOJ FAZI KADIOHIRURGIJE KOJE UKLJUČUJE EKSTRAKORPOREALNU CIRKULACIJU

2025-11-02T16:33:36+01:00

Uvod: Da bismo stekli uvid u ulogu i važnost upalnih i imunoloških markera u sveobuhvatnoj proceni imunološkog odgovora pacijenta na hirurški zahvat, naša studija se fokusirala na istraživanje preoperativne i postoperativne dinamike serumskih nivoa SAA, CRP i udela HLA-DR⁺CD14⁺monocita, CD14⁺ monocita, and pro-inflammatornih monocita CD16⁺CD14⁺ kod pacijenata koji su bili podvrgnuti operaciji srca uz pomoć ekstrakorporalne cirkulacije (on-pump).

Metode: U Centru za srce Kliničkog centra Univerziteta u Sarajevu provedena je opservacijska, prospektivna studija na 53 pacijenta podeljena u 3 starosne grupe: 50 – 59, 60 – 69 i 70 – 80 godina. Nivoi CRP i SAA u serumu su kvantitativno određeni imunonefelometrijom. Istovremeno je primenjena tehnologija protočne citometrije za merenje udela HLA-DR⁺CD14⁺monocita, CD14⁺ monocita, and pro-inflammatornih monocita CD16⁺CD14⁺

Rezultati: Izmerene vrednosti CRP, SAA, udeo monocita CD14⁺ monocita i udeo proinflamatornih monocita CD16⁺CD14⁺ su značajno povećane postoperativno u odnosu na preoperativne vrednosti (p<0,05). Udeo HLA-DR⁺CD14⁺monocita je niži postoperativno u odnosu na preoperativne vrednosti (p<0,001). Nadalje, nema značajnih polnih razlika u preoperativnim ili postoperativnim parametrima (p>0,05), sa izuzetkom preoperativnog udela monocita (CD14⁺) (p<0,05). Analiza starosnih razlika ukazuje da među definisanim starosnim grupama nije bilo značajnih promena u posmatranim preoperativnim i postoperativnim parametrima (p>0,05).

Zaključak: Rano praćenje upalnih i imunoloških markera u postoperativnoj fazi moglo bi biti korisno za zdravstvene profesionalce za provođenje brzih intervencija usmjerenih na ublažavanje negativnih ishoda.

Procena kvaliteta biohemijskih analita zasnovana na metodi Six Sigma: Komparativna analiza standarda Kliničkih laboratorijskih poboljšanja iz 1988. i 2024. godine

2025-11-02T16:34:51+01:00

Background: Laboratories have a responsibility to produce accurate and reliable results to ensure patient safety and meet quality standards. Within the Six Sigma quality management framework, different approaches may emerge depending on the total allowable error (TEa) limits defined by various standards. This study aimed to compare analyte sigma levels using CLIA 1988 and CLIA 2024 criteria, determine appropriate quality control procedures based on Westgard multirules, and identify potential sources of error using the Quality Goal Index.

Methods: Sigma values for 17 biochemical analytes were calculated based on internal and external quality control data using the formula (TEa – bias)/CV. The Quality Goal Index (QGI) was determined using the formula bias/(1.5×CV). All analytes were evaluated at the Karabuk Public Health Laboratory between November 2024 and March 2025.

Results: Amylase (Levels 1 and 2), total bilirubin (Level 1), high-density lipoprotein cholesterol (Levels 1 and 2), creatine kinase (Levels 1 and 2), and lactate dehydrogenase (Levels 1 and 2) demonstrated world-class sigma performance according to both CLIA 1988 and CLIA 2024 standards. However, a decline in sigma values was observed when calculated using the more stringent CLIA 2024 limits.

Conclusions: The comparison of CLIA 1988 and CLIA 2024 standards demonstrated that stricter TEa limits can significantly impact sigma performance. While some analytes retained world-class performance, others showed a notable decline, requiring enhanced quality control measures. These findings emphasize the need for laboratories to periodically reassess test performance in light of evolving regulatory standards to ensure continued analytical reliability and patient safety.

Causal effects of inflammatory cytokines on hemangioma mediated by blood metabolites: a two-step Mendelian randomization study

2025-11-02T15:27:09+01:00

Background: Hemangioma is the most prevalent infantile vascular tumor. The state of inflammation and metabolism may contribute to the occurrence and development of hemangioma, but their causal relationships have not been clearly elucidated. In this study, via Mendelian randomization (MR) analysis, we aimed to investigate the causal effect of inflammatory cytokines and blood metabolites on hemangioma, and to explore the potential mediating role of metabolites.

Methods: Applying large-scale genome-wide association studies (GWAS) dataset, we applied two-sample MR to infer causal relationships among 91 inflammatory cytokines, 1091 blood metabolites and 309 metabolite ratios，and hemangioma. In addition, a two-step MR was used to assess the potential mediating role of metabolites. Functional enrichment was also performed to explore the biological pathways involved.

Results: 9 cytokines exhibited significant causal effects on hemangioma. Cytokines such as CCL20, IFN-γ, Eotaxin and TRANCE were associated with an increased risk, while IL12B, CXCL11, TGF-α, OSM and IL17A were inversely associated. Additionally, 52 blood metabolites and metabolite-ratios were discovered to have causal effects on hemangioma. 18 metabolites and metabolite-ratios were associated with an elevated risk of hemangioma, whereas 34 metabolites and metabolite-ratios appeared to be protective factors. Mediation analysis further identified specific metabolites, such as Gamma-glutamylvaline, as mediators in cytokine-hemangioma pathways, suggesting that they might modulate cytokine-driven tumorigenesis.

Conclusion: As the first MR study focused on hemangioma, we identified key cytokines and metabolites which might exert a causal effect on hemangioma, with several metabolites functioning as intermediators in cytokine-induced tumorigenesis process. The complex interaction between inflammation and metabolism in hemangioma was revealed, laying a foundation for future studies to explore potential targeted treatments.

Analyze correlation of inflammatory chemokine CXCL8 and lymphoid axillary lymph node metastasis in breast cancer

2025-11-02T16:37:23+01:00

Background: In this study, we analyzed the relationship between serumchemokine CXC ligand 8 (CXCL8) and breast cancer (BC) and the predictive effect on axillary lymph node metastasis.

Methods: 121 BC patients and 104 healthy controls were included, and serum CXCL8 was detected by ELISA to compare the differences in the levels of CXCL8 and tumor markers in the two study groups. Pathological examinations revealed that 36 of the patients had axillary lymph node metastasis. To further evaluate the diagnostic value, the ROC curve was employed to analyze the ability of CXCL8, tumor marker and combined color Doppler ultrasound blood flow richness grade (Adler grade) to assess axillary lymph node metastasis in BC patients.

Results: Serum CXCL8 CEA, CA153, and CA27.29 were higher in BC patients than in controls (P < 0.05). Patients with lymph node metastasis had higher levels of CXCL8 CEA, CA153, and CA27.29 (P < 0.05). The AUC of CXCL8+tumor markers+Adler grade for the diagnosis of lymph node metastasis was 0.903 (95% CI: 0.850-0.957), with a sensitivity of 86.11% and a specificity of 82.35% (P < 0.001).

Conclusion: High expression of CXCL8 is closely associated with BC lymph node metastasis.

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2025-11-02T15:27:09+01:00

Da bi se proučila korelacija između nivoa laktata u krvi i neonatalne hiperbilirubinemije i da se predvidi ozbiljnost NHB kod novorođenčadi

2025-11-02T16:39:01+01:00

Cilj: Proučiti korelaciju između nivoa laktata u krvi i hiperbilirubinemije kod novorođenčadi i analizirati prediktivnu vrednost nivoa laktata u krvi za težinu NHB kod novorođenčadi.

Metode: Ukupno 123 dece sa dijagnozom neonatalne hiperbilirubinemije (NHB) primljene u odeljenje za akušerstvo od oktobra 2021. do oktobra 2022. godine izabrano je kao NHB grupa, a prema težini bolesti podeljeni su na blage, umerene i teške. Pedeset zdravih novorođenčadi rođenih u odeljenju za akušerstvo izabrano je kao kontrolna grupa. Upoređeni su nivoi glukoza-6-fosfat dehidrogenaze (G6PD), izoenzima kreatin kinaze (CK-MB), β2-mikroglobulina (β2-MG), aspartat aminotransferaze (AST), mlečne kiseline u krvi i ukupnog bilirubina u serumu. Pearsonova korelacijska analiza korišćena je za analizu korelacije između nivoa laktata u krvi i nivoa bilirubina, a ROC kriva je korišćena za određivanje prediktivne vrednosti nivoa laktata u krvi za težinu NHB kod novorođenčadi.

Rezultati: NHB grupa je imala značajno viši nivo AST, CK-MB, β2-MG, mlečne kiseline u krvi i ukupnog bilirubina od NHB grupe (P < 0,05). Nivo G6PD je bio znatno niži (P < 0,05). Prema Pearsonu, nivo mlečne kiseline u krvi bio je pozitivno povezan sa ukupnim nivoom bilirubina (r = 0,604, P < 0,001). ROC kriva je pokazala da je AUC nivoa mlečne kiseline u krvi u predviđanju ozbiljnosti NHB bio 0,873, što je bilo više nego kod ukupnog bilirubina (P < 0,05).

Zaključci: Novorođenčad sa NHB imaju viši nivo AST, CK-MB, β2-MG, laktata u krvi i ukupnog bilirubina u serumu, dok su niži nivoi G6PD. Nivo laktata u krvi u serumu je pozitivno povezan sa ukupnim nivoom bilirubina, koji se može koristiti za posmatranje težine NHB kod novorođenčadi.

Correlation of Serum sIL-2R, VEGF, and ES with Estrogen Levels in Papillary Thyroid Carcinoma Patients and Their Predictive Value for Postoperative Recurrence

2025-11-02T16:39:50+01:00

Objective: To explore the correlation of serum soluble interleukin-2 receptor (sIL-2R), vascular endothelial growth factor (VEGF), and endostatin (ES) with estrogen levels in papillary thyroid carcinoma (PTC) patients, and to assess the predictive efficacy of these biomarkers for PTC diagnosis and postoperative recurrence.

Methods: From March 2023 to March 2024, 132 newly diagnosed PTC patients and 128 healthy controls were enrolled. Serum sIL-2R, VEGF, and ES levels were quantified using enzyme-linked immunosorbent assay (ELISA), while estrogen levels (estrone [E1], estradiol [E2], estriol [E3]) were measured via chemiluminescent immunoassay. Patients were followed postoperatively for one year to monitor recurrence events, including local recurrence, lymph node metastasis, and distant metastasis. The diagnostic performance of the combined model was evaluated using receiver operating characteristic (ROC) curve analysis, and Pearson correlation analysis was conducted to examine the relationship between biomarkers and estrogen levels.

Results: Compared to controls, PTC patients exhibited significantly elevated serum sIL-2R, VEGF, and ES levels (P<0.05). The combined detection of these biomarkers demonstrated a sensitivity of 80.30% and specificity of 78.91% (AUC=0.8526) for PTC diagnosis. Additionally, E1 and E2 levels were significantly higher in PTC patients (P<0.05) and showed positive correlations with sIL-2R, VEGF, and ES (P<0.05), whereas E3 levels changed insignificantly (P>0.05). Recurrent patients had significantly higher sIL-2R, VEGF, and ES levels than non-recurrent patients (P<0.05). The combined predictive model for recurrence achieved a sensitivity of 96.88% and specificity of 61.00% (AUC=0.8494).

Conclusion: Elevated serum sIL-2R, VEGF, and ES levels in PTC patients indicate that their combined assessment may serve as a sensitive and specific tool for PTC diagnosis and postoperative recurrence risk stratification.

Klinička vrednost inflamatornih faktora u proceni prognoze pacijenata sa akutnom mijeloičnom leukemijom

2025-11-19T04:10:45+01:00

Background: To assess the importance of inflammatory factors in predicting outcomes in individuals diagnosed with acute myeloid leukemia.

Methods: Between July 2017 and December 2019, a total of 100 patients with a recent diagnosis of acute myeloid leukemia (AML) were recruited from the Hematology Department of our hospital’s Cancer Center and assigned to the AML group. Additionally, 60 individuals with no underlying health conditions who underwent standard medical checkups during the same period were selected as the control group. Serum levels of IL-2, IL-4, IL-17A, TNF-α, IFN-γ, and LIF were measured through ELISA. All participants in the AML group were followed up for three years. Based on the European Leukemia Network (ELN) genetic risk stratification criteria, patients were categorized into favorable, intermediate, and adverse prognosis subgroups. Inflammatory marker profiles were then compared among these subgroups.

Results: Compared to healthy individuals, the AML group presented significantly increased serum concentrations of IL-4, IL-17A, and TNF-α, while levels of IL-2, IFN-γ, and LIF were significantly decreased (P < 0.05). Upon stratifying patients by prognostic classification, those in the favorable and intermediate prognosis categories exhibited notably lower IL-4, IL-17A, and TNF-α levels relative to the poor prognosis group (all P < 0.05). In contrast, levels of IL-2, IFN-γ, and LIF were notably elevated in the subgroup with favorable prognostic profiles (all P < 0.05). Additional analysis of cytokine expression indicated that increased levels of IL-4, IL-17A, and TNF-α were linked to worse 3-year survival performance (P < 0.05). Conversely, higher expression of IL-2, IFN-γ, and LIF was significantly associated with better long-term survival outcomes relative to patients with reduced expression levels (P < 0.05).

Conclusion: The abnormal levels of IL-4, IL-17A, TNF-a, IFNγ and LIF in patients with acute myeloid leukemia are increased, while the abnormal levels of IL-2, IFNγ and LIF are decreased, which has certain guiding effect on prognosis assessment and can be used as auxiliary indicators for prognosis assessment of AML.

Investigating the Causal Association Between Plasma Lipids and the Risk of Squamous Cervical Cancer: A Two-Sample Mendelian Randomization Study

2025-11-02T15:27:10+01:00

Purpose: This study aimed to investigate the causal relationship between plasma lipid levels—total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C)—and the risk of squamous cervical cancer (SCC) using Mendelian Randomization (MR).

Materials and Method: Genome-wide association study (GWAS) data for plasma lipid traits were obtained from the Global Lipids Genetics Consortium (GLGC), and SCC outcome data were sourced from the FinnGen consortium. The primary analysis was conducted using the inverse variance weighted (IVW) method, supported by MR-Egger regression, weighted median, and weighted mode approaches. Sensitivity analyses were performed to assess the robustness of the results, and the Steiger test was used to evaluate the directionality of the associations.

Results: The IVW analysis revealed that higher plasma levels of TC (OR: 1.777; 95% CI: 1.118–2.825; p = 0.015) and LDL-C (OR: 1.674; 95% CI: 1.013–2.767; p = 0.044) were associated with an increased risk of SCC. No significant associations were found between TGs (OR: 0.644; 95% CI: 0.343–1.212; p = 0.173) or HDL-C (OR: 1.050; 95% CI: 0.616–1.790; p = 0.857) and SCC.

Conclusions: This study provides evidence of a causal relationship between elevated plasma TC and LDL-C levels and a higher risk of SCC, highlighting a novel aspect of SCC etiology. These findings may inform further functional and clinical research in the progression of SCC.

Clinical Utility of Jugular Venous Blood Gas Biomarkers for Assessing Traumatic Brain Injury Severity and Predicting ICU Stay Duration: A Biochemical and Predictive Modeling Approach

2025-11-02T15:27:10+01:00

Background: Biochemical monitoring of cerebral oxygen metabolism is essential in the management of traumatic brain injury (TBI). Jugular venous blood gas (JVBG) analysis provides real-time data reflecting cerebral biochemical states, offering a valuable window into brain oxygenation and metabolic demands. This study aimed to evaluate JVBG biochemical markers as indicators of TBI severity and as predictors of prolonged intensive care unit (ICU) stay, emphasizing their integration into machine learning models for clinical utility.

Methods: A retrospective analysis was conducted on 100 ICU-admitted TBI patients. Serial measurements of JVBG parameters—jugular venous oxygen saturation (SjvO₂), partial pressure of oxygen (PjO₂), arteriovenous oxygen difference (AVDO₂), and oxygen content difference (AVDL)—were performed over five days. Spearman correlation and random forest algorithms were employed to assess relationships between JVBG biomarkers, clinical severity (via Glasgow Coma Scale), and ICU duration.

Results: Patients with more severe TBI exhibited significantly reduced SjvO₂ and PjO₂, and elevated AVDO₂ and AVDL (p < 0.001). Strong correlations were found between JVBG markers and clinical severity scores. A multivariable prediction model incorporating age, SaO₂, and PaCO₂ at one day post-admission yielded excellent predictive performance for prolonged ICU stay (AUC = 0.974; sensitivity = 100%; specificity = 94.8%).

Conclusion: JVBG biomarkers serve as clinically informative biochemical indicators for assessing TBI severity and forecasting ICU duration. Their integration into predictive algorithms may enhance precision in neurocritical care and support biochemical decision-making in intensive care settings.

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2025-11-02T16:36:35+01:00