Antimicrobial activity evaluation of black currant (Ribes nigrum L.) variety Čačanska crna juice and extract :

Milica Trajković; Dušanka Kitić; Tatjana Mihajilov-Krstev; Katarina Šavikin; Milica Randjelović; Milica  Milutinovic; Suzana Branković; Nemanja Kitić; Bojana  Miladinovic

doi:10.5937/afmnai40-41954

Milica Trajković Medicinski fakultet, Univerzitet u Nišu
Dušanka Kitić Medicinski fakultet, Univerzitet u Nišu https://orcid.org/0000-0001-8551-274X
Tatjana Mihajilov-Krstev Prirodno-matematički fakultet Univerzitet u Nišu
Katarina Šavikin Institut za proučavanje lekovitog bilja Dr Josif Pančić https://orcid.org/0000-0002-2086-9593
Milica Randjelović Medicinski fakultet, Univerzitet u Nišu https://orcid.org/0000-0002-6951-9957
Milica Milutinovic Medicinski fakultet, Univerzitet u Nišu https://orcid.org/0000-0002-9895-3840
Suzana Branković Medicinski fakultet, Univerzitet u Nišu
Nemanja Kitić Medicinski fakultet, Univerzitet u Nišu
Bojana Miladinovic

DOI: https://doi.org/10.5937/afmnai40-41954

Ključne reči: Crna ribizla, bobičasto voće, antioksidans, antimikrobna aktivnost, antocijani.

Sažetak

Cilj: Ovo istraživanje je imalo za cilj da proceni i kvantifikuje antimikrobnu aktivnost liofilizovanog voćnog soka (BCLJ) i ekstrakta ostalog nakon ceđenja soka (BCLW) dobijenog od crne ribizle (Ribes nigrum L.) sorte Čačanska crna.

Materijal I metoda: Studija je sprovedena korišćenjem četiri Gram (+) (Bacillus cereus, Listeria monocitogenes, Staphilococcus aureus, Enterococcus faecalis) i pet Gram (-) bakterija (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Proteus mirabilis, Enterobacter aerogenes), kao I jedne gljivice (Candida albicans). Kao standardi korišćeni su cijanidin-3-O-glukozid, delfinidin-3-O-rutinozid i delfinidin-3-O-glukozid prisutni u crnoj ribizli, pa je drugi cilj bio da se utvrdi njihov uticaj na ukupnu antimikrobnu aktivnost.

Rezultati: Ispitani uzorci su pokazali umerenu antimikrobnu aktivnost. Inhibitorni efekat BCLJ se pokazao na sve Gram (+) bakterije (B. cereus, E. faecalis, S. aureus), osim na L. monocitogenes, za koje ekstrakti nisu bili efikasni. Primećeno je da BCLJ nije delovao na rast Gram (-) bakterija. Ekstrakt ostatka crne ribizle, s druge strane, pokazao se efikasnim na Gram (+) kao i na Gram (-) bakterije. Rezultati minimalnih inhibitornih koncentracija (MIC (MFC) BCLJ i BCLW bili su 100 mg/mL i minimalnih baktericidnih koncentracija (MBC) su bili veći od 100 mg. MIC/MBC (MFC) standarda je bio 0,13-0,5 mg/mL.

Zaključak: Rezultati pokazuju da se ovi liofilizati crne ribizle mogu potencijalno koristiti kao antimikrobni agensi.

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Procena antimikrobne aktivnosti soka i ekstrakta crne ribizle (Ribes nigrum L.) sorte Čačanska crna

Antimikrobna aktivnost soka i ekstrakta crne ribizle

Sažetak

Reference