UTICAJI ETANOLNOG EKSTRAKTA LISTA ANETHUM GRAVEOLENS L. NA KONTRAKTILNU AKTIVNOST IZOLOVANOG ILEUMA I TRAHEJE PACOVA
Abstract
Mirođija (Anethum graveolens L., Apiaceae) se vekovima koristi kao začin, ali i kao lekovito sredstvo za gastrointestinalne probleme u tradicionalnoj medicini. Cilj ove studije jeste da proceni efekte etanolnog ekstrakta lista Anethum graveolens L. (AGEE) na kontraktilnu aktivnost izolovanog ileuma, kao i traheje pacova. AGEE je dobijen ultrazvučnom ekstrakcijom iz osušenih i usitnjenih listova kultivisane mirođije. Ova studija pratila je efekte AGEE na spontane, KCl (80 mM), acetilholinom i CaCl2 indukovane kontrakcije izolovanog ileuma pacova, kao i kontraktilnu aktivnost izolovane traheje pacova indukovane karbaholom i KCl (80 mM). Rezultati pokazuju da AGEE indukuje statističi značajnu (p < 0,01) i kontrakciono zavisnu relaksaciju spontanih i indukovanih kontrakcija izolovanog ileuma. Takođe, AGEE statističi značajno (p < 0,01) umanjuje, proporcionalno primenjenoj koncentraciji, kontraktilne efekte karbahola i KCl na izolovanu traheju pacova. Naši rezultati pokazuju da AGE smanjuje kontraktilnost glatke muskulature izolovanog ileuma i traheje pacova.
References
Abbasi-Oshaghi E, Khodadadi I, Tavilani H, Mirzaei F, Goodarzi MT. Dill-normalized liver lipid accumulation, oxidative stress, and low-density lipoprotein receptor levels in high cholesterol fed hamsters. ARYA Atheroscler 2018; 14(5):218-24. [CrossRef] [PubMed]
Al-Sheddi ES, Al-Zaid NA, Al-Oqail MM, Al-Massarani SM, El-Gamal AA, Farshori NN. Evaluation of cytotoxicity, cell cycle arrest and apoptosis induced by Anethum graveolens L. essential oil in human hepatocellular carcinoma cell line. Saudi Pharm J 2019; 27(7):1053-60. [CrossRef] [PubMed]
Brading AF. How do drugs initiate contraction in smooth muscles?. Trends Pharmacol Sci 1981; 2:261-5. [CrossRef]
Brankovic S, Kitic D, Radenkovic M, Veljkovic S, Jankovic T, Savikin K, et al. Spasmolytic activity of the ethanol extract of Sideritis raeseri spp. raeseri Boiss. & Heldr. on the isolated rat ileum contractions. J Med Food 2011; 14(5):495-8. [CrossRef] [PubMed]
Brankovic SV, Kitic DV, Radenkovic MM, Veljkovic SM, Golubovic TD. Calcium blocking activity as a mechanism of the spasmolytic effect of the essential oil of Calamintha glandulosa Silic on the isolated rat ileum. Gen Physiol Biophys 2009; 28:174-8. [PubMed]
Castro LM, Pinto NB, Moura MQ, Villela MM, Capella GA, Freitag RA, et al. Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae. Braz J Biol 2020; 81:183-8. [CrossRef] [PubMed]
Gilani AH, Khan AU, Ali T, Ajmal S. Mechanisms underlying the antispasmodic and bronchodilatory properties of Terminalia bellerica fruit. J Ethnopharmacol 2008; 116(3):528-38. [CrossRef] [PubMed]
Gočmanac-Ignjatović M, Kitić D, Kostić M, Miladinović B, Milutinović M, Veljković M, et al. Spasmolytic effect of Anethum graveolens L. methanol extract on isolated rat ileum. Acta Medica Medianae 2015; 54(2):5-10. [CrossRef]
Goodarzi MT, Khodadadi I, Tavilani H, Oshaghi EA. The role of Anethum graveolens L. (Dill) in the management of diabetes. J Trop Med 2016; 2016:1098916. [CrossRef] [PubMed]
Hekmatzadeh SF, Bazarganipour F, Allan H, Aramesh S, Mohammadi J. Effects of boiled dill seed on anxiety during labor: a randomized clinical trial. Chin J Integr Med 2020; 26(2):100-5. [CrossRef] [PubMed]
Hosseinzadeh H, Karimi G, Ameri M. Effects of Anethum graveolens L. seed extracts on experimental gastric irritation models in mice. BMC Pharmacol 2002; 2(1):1-5. [CrossRef] [PubMed]
Idiz C, Aysan E, Elmas L, Bahadori FA, Idiz UO. Effectiveness of Anethum graveolens L. on antioxidant status, thyroid function and histopathology. Acta Endocrinol-Buch 2018; 14(4):447. [CrossRef] [PubMed]
Jafarzade P, Mehran SMM, Moladoust H, Norasfard MR, Ghorbani A, Abedinzade M. Effect of hydroalcoholic extract of anethum graveolens l. Seed on tracheal smooth muscle contractions in male rats. J Maz Univ Med 2018; 28(160):146-50.
Jana S, Shekhawat GS. Anethum graveolens: An Indian traditional medicinal herb and spice. Pharmacogn Rev 2010; 4(8):179-84. [CrossRef] [PubMed]
Karaki H, Weiss GB. Calcium release in smooth muscle. Life Sci 1988; 42(2):111-22. [CrossRef] [PubMed]
Mirmolaee ST, Hekmatzadeh SF, Kazemnazhad A, Aidenlou F, Shamsi M. Evaluating the effects of dill (Anethum graveolens) seed on the duration of active phase and intensity of labour pain. J Herb Med 2015; 5(1):26-9. [CrossRef]
Mohammad IH. Use of Anethum graveolens in the management of patients with irritable bowel syndrome. Mustansiriya Medical Journal 2012; 11(1):94-8.
Naseri MG, Heidari A. Antispasmodic effect of Anethum graveolens fruit extract on rat ileum. Int J Pharmacol 2007; 3(3):260-4. [CrossRef] [PubMed]
Omar E, Pavlović I, Drobac M, Radenković M, Branković S, Kovačević N. Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst.) Chiov. (Poaceae) essential oil. Ind Crop Prod 2016; 91:249-54. [CrossRef]
Oshaghi EA, Khodadadi I, Tavilani H, Goodarzi MT. Aqueous extract of Anethum graveolens L. has potential antioxidant and antiglycation effects. Iran J Med Sci 2016; 41(4):328. [PubMed]
Saikia B, Barua CC, Haloi P, Patowary P. Anticholinergic, antihistaminic, and antiserotonergic activity of n-hexane extract of Zanthoxylum alatum seeds on isolated tissue preparations: An ex vivo study. Indian J Pharmacol 2017; 49(1):42-8. [CrossRef] [PubMed]
Saini N, Singh GK, Nagori BP. Spasmolytic potential of some medicinal plants belonging to family Umbelliferae: A review. Int J Res Ayurveda Pharm 2014; 5(1):74-83. [CrossRef]
Shafaei N, Barkhordar SM, Rahmani F, Nabi S, Idliki RB, Alimirzaei M, et al. Protective effects of Anethum graveolens seed’s oil nanoemulsion against cadmium-induced oxidative stress in mice. Biol Trace Elem Res 2020; 198(2):583-91. [CrossRef] [PubMed]
Souza FV, da Rocha MB, de Souza DP, Marçal RM. (−)-Carvone: Antispasmodic effect and mode of action. Fitoterapia 2013; 85:20-4. [CrossRef] [PubMed]
Wattanathorn J, Ohnon W, Thukhammee W, Muchmapura S, Wannanon P, Tong-Un T. Cerebroprotective effect against cerebral ischemia of the combined extract of Oryza sativa and Anethum graveolens in metabolic syndrome rats. Oxid Med Cell Longev 2019; 2019:9658267. [CrossRef] [PubMed]
Yasin M, Janbaz KH, Imran I, Gilani AU, Bashir S. Pharmacological studies on the antispasmodic, bronchodilator and anti‐platelet activities of Abies webbiana. Phytother Res 2014; 28(8):1182-7. [CrossRef] [PubMed]