CORRELATION ANALYSIS OF PITUITARY LUTEINIZING AND SOMATOTROPIC CELLS IN MALE CADAVERS DURING AGING

Jovana Čukuranović Kokoris; Jelena Marković Filipović; Miodrag Đorđević; Braca Kundalić; Vesna Stojanović; Milena Trandafilović; Ivana Graovac; Verica Milošević; Rade Čukuranović

Jovana Čukuranović Kokoris University of Niš, Faculty of Medicine, Department of Anatomy, Niš
Jelena Marković Filipović University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad
Miodrag Đorđević University of Niš, Faculty of Medicine, University Clinical Center Niš, Clinic for Endocrine Surgery, Niš
Braca Kundalić University of Niš, Faculty of Medicine, Department of Anatomy, Niš
Vesna Stojanović University of Niš, Faculty of Medicine, Department of Anatomy, Niš
Milena Trandafilović University of Niš, Faculty of Medicine, Department of Anatomy, Niš
Ivana Graovac University of Niš, Faculty of Medicine, Department of Anatomy, Niš
Verica Milošević University of Niš, Faculty of Medicine, Department of Anatomy, Niš
Rade Čukuranović University of Niš, Faculty of Medicine, Department of Anatomy, Niš

Keywords: aging, men, immunoreactive luteinizing cells, immunoreactive somatotropic cells, immunohistomorphometry

Abstract

This manuscript focused on examining the correlation between immunoreactive luteinizing (LH) and immunoreactive somatotropic (GH) cells in men during ageing. Anti-LH and anti-GH are the antibodies used to label the mentioned pituitary cells in 14 male cadavers. The cells identified in this way were analyzed with ImageJ. The obtained results were statistically analyzed using the SPSS statistical software package. The results of the morphometric analysis showed that during ageing, the surface area of LH and GH cells increased significantly (p < 0.05), and that the nuclear-cytoplasmic ratio decreased, and that the obtained changes were particularly significant (p < 0.05) in elderly cadavers over 70 years of age. These results showed that after the mentioned period, there was a hypertrophy of the examined cells. The resulting changes were of a functional nature and showed that cadavers after the age of 70 have a significantly reduced hormonal capacity. Based on this, it can be concluded that the investigated morphometric parameters of gonadotropic LH and GH cells correlate significantly, which indicates the parallel occurrence of adaptation and compensatory mechanisms in these cells in men during ageing.

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