CHARACTERISTICS OF IMMUNE RESPONSE DURING HERPES SIMPLEX VIRUS INFECTION IN CHILDHOOD
Abstract
The infection with the herpes simplex virus (HSV) basically implies the chronic activation of the immune system. Interferon gamma (IFN-γ) and Interleukin 4 (IL-4) are part of the mechanisms that control the immune system's response to recurrent herpes simplex virus. The research included 40 children (2−15 years old) with clinical herpes simplex virus infection. Routine laboratory tests were performed on the patients: leukocyte count, creatinine-kinase-CPK, oxidative stress (nitro-blue tetrazolium), NBT test, lactate-LDH dehydrogenation, IFN-γ, IL-4 levels in serum were measured by ELISA test. The serological test for HSV type I virus was positive in all patients. A high level of LDH, CPK was detected as well as a low ability to reduce NBT. An increased level of IFN-γ, IL-4 was observed compared to the control group of patients (who did not have clinical manifestations of herpes virus infection). Patients with a high concentration of IFN-γ are associated with a low concentration of NBT-test. During infection of virus herpes simplex, an immune response is activated (lymphocyte Th1and Th2 type are stimulated). Different clinical manifestations are based on a certain type of immune response. Our results presented the dominance of the Th1 type of response over the Th2 type. The production of IFN gamma was higher compared to IL4. Oxidative stress parameters were also associated with the dominant Th1 type of immune response. This is all important for prognosis, prevention and therapy.
References
Arena A, Bisignano C, Stassi G, Filocamo A, Mandalari G.Almond Skin Inhibits HSV-2 Replication in Peripheral Blood Mononuclear Cells by Modulating the Cytokine Network Molecules 2015; 20: 8816-22. [CrossRef] [PubMed]
Aslanadenh J, Helm KF, Espy MJ, Muller SA, Smith TF. Detection of HSV-specific DNA in biopsy tissue of patients with erythema multiforme by polymerase chain reaction. Br J Dermatol 1993; 126(1):19-23. [CrossRef] [PubMed]
Atedzoe BN, Menezes J, Addario MD, Xu J, Ongradi J, Ahmad A. Modulatory effects of human herpes virus-7 on cytokine synthesis and cell proliferation in human peripheral blood mononuclear cell cultures. J Leukoc Biol 1999; 66(5):822-28. [CrossRef] [PubMed]
Bastuji-Garin S, Rzany B, Stern RS, et al. Clinical classification of cases of toxic epidermal necrolysis: Stevens-Johnson syndrome and erythema multiforme. Arch Dermatol 1993; 129(1):92–6. [CrossRef] [PubMed]
Bork K, Bruninger W. Increasing incidence of eczema herpeticum: analysis of seventy-five cases. J Am AcadDermatol 1988; 19:1024–9. [CrossRef] [PubMed]
Boulanger MJ, Chow DC, Breynova E, Martick M, Stanford G, Nocholas J, Garcia KC. Molecular mechanisms for viral mimicry of a human cytokine: Activation of gp 130 by HHV-8 interleukin-6. J Mol Biol 2004; 335(2): 641–54. [CrossRef] [PubMed]
Brice SL, Krzemien D, Weston WL, Huff JC. Detection of herpes simplex virus DNA in cutaneous lesions of erythema multiforme. J Invest Dermatol 1989; 93(1):183–7. [CrossRef] [PubMed]
Brice SL, Leahy MA, Ong L, et al. Examination of non-involved skin, previously involved skin, and peripheral blood for herpes simplex virus DNA in patients with recurrent herpes-associated erythema multiforme. J CutanPathol 1994; 21(5):408–12. [CrossRef] [PubMed]
Corry DB, Kheradmand F, Luong A, Pandit L. Immunological mechanisms of airway diseases and pathways to therapy. In: Rich RR, editor. Clinical Immunology: principles and practice. 4th ed. Philadelphia: Elsevier Saunders; 2013. [CrossRef]
Cunningham AL, Merigan TC. Interferon production appears to predict time of recurrence of herpes labialis. J Immunol 1983; 130(5):2397–9. [CrossRef] [PubMed]
Green JA, Weiss PN, Yeh TJ, Spruance SL. Immune specific interferon production by peripheral blood mononuclear leukocytes from patients with primary and recurrent oral-labial herpes simplex virus infections. J Med Virol 1985; 16(3):297–305. [CrossRef] [PubMed]
Green JA, Yeh TJ, Overall JC. Sequential production of IFN-a and immunespecific IFN-by human mononuclear leukocytes exposed to herpes simplex virus. J Immunol 1981; 127(3):1192–6. [CrossRef] [PubMed]
Heiligenhaus A, Bauer D, Zheng M, Mrzyk S, Steuhl KP.CD4+ T-cell type 1 and type 2 cytokines in the HSV-1 infected cornea. Graefes Arch Clin Exp Ophthalmol. 1999;237(5):399-406. [CrossRef] [PubMed]
James SH, Whitley RJ. Treatment of herpes simplex virus infections in pediatric patients: current status and future needs Clin Pharmacol 2010;88(5):720-4. [CrossRef] [PubMed]
Jie Zhang, Huan Liu, Bin Wei. Immune response of T cells during simplex virus type 1 (HSV-1) infection. J. ZhejlangUniv-Sci B 2017;18(4):277-88. [CrossRef] [PubMed]
Johnston RB, Kitagawa S. Molecular basis for the enhanced respiratory burst of activated macrophages. Fed Proc1985; 44(14):2927-32. [PubMed]
Khalil I, Lepage V, Douay C, et al. HLA-DBQB1*0301 allele is involved in susceptibility to erythema multiforme. J Invest Dermatol 1991; 97(4): 697–700. [CrossRef] [PubMed]
Khanna K.M, Lepisto A.J, Hendricks R.L. Immunity to latent viral infection: many skirmishes but few fatalities Trends in Immunology 2004;25(5):230-4 . [CrossRef] [PubMed]
Kuo Y, Lin C. Recurrent herpes simplex virus type 1 infection precipitated by the impaired production of interleukin-2, a-interferon, and cell mediated immunity. J Med Virol 1990; 31(3):183–9. [CrossRef] [PubMed]
Lawrence R. Stanberry, Anthony L. Cunningham, Adrian Mindel, Laurie L. Scott, Spotswood L. Spruance, Fred Y. Aoki, and Charles J. Lacey. Prospects for control of Herpes Simplex Virus Disease thorough Immunization Clinical Infection Disease 2000;30(3):549-66. [CrossRef] [PubMed]
Leaute-Labreze C, Lamireau T, Chawki D, Maleville J, TaiebA.Diagnosis, classification, and management of erythema multiforme and Steves-Johnson syndrome Arch Dis Child 2000;83(4):347-52. [CrossRef] [PubMed]
Lekstrom-Himes JA, Leblanc RA, PesnicakL,Godleski M, Straus SEGamma Interferon Impedes the Establishment of Herpes Simplex Virus Type 1 Latent Infection but Has No Impact on Its Maintenance or Reactivation in Mice. J Virol 2000;74(14): 6680–3. [CrossRef] [PubMed]
Meinl E, Fickensher H, Thome M, Tschoopp J, Fleckenstein B. Anti-apoptotic strategies of lymphotropic viruses. Immunol Today 1998;19(10):474-9. [CrossRef] [PubMed]
Mikloska Z, Kesson AM, Penfold ME, Cunningham AL. Herpes simplex virus protein targets for CD4 and CD8 lymphocyte cytotoxicity in cultured epidermal keratinocytes treated with interferon-g. J Infect Dis 1996; 173(1):7–17. [CrossRef] [PubMed]
Mogensen TH, Melchjorsen J, Malmgaard L, Casola A, Paludan SR. Suppression of proinflammatory cytokine expression by herpes simplex virus type 1. J. Virol 2004; 78(11): 5883–90. [CrossRef] [PubMed]
Mullick J, Kadam A, Sahu A Herpes and pox viral complement control proteins: "the mask of self". Trends Immunol 2003;24(9):500-7. [CrossRef] [PubMed]
O’Reilly RJ, Chibbaro A, Anger E, Lopez C. Cell-mediated immune responses in patients with recurrent herpes simplex infections. J Immunol 1977; 118(3):1095–102. [CrossRef] [PubMed]
Park B, Fikrig S, Smitwick E. Infection and nitrobluetetrasolium reduction by neutrophils Lancet 1968;2(7567):532-4. [CrossRef] [PubMed]
Rasmussen SB, Sørensen LN, Malmgaard L, Ank N, Baines JD, Chen ZJ, et al. Type I interferon production during herpes simplex virus infection is controlled by cell-type-specific viral recognition through Toll-like receptor 9, the mitochondrial antiviral signaling protein pathway, and novel recognition systems. J Virol. 2007; 81(24): 13315–24. [CrossRef] [PubMed]
Schofield JK, Tatnall FM, Leigh IM. Recurrent erythema multiforme: clinicalfeatures and treatment in a large series of patients. Br J Dermatol 1993; 128(5):542–5. [CrossRef] [PubMed]
Shillitoe EJ, Wilton JMA, Lehner T. Sequential changes in cell-mediated immune responses to herpes simplex virus after recurrent herpetic infection in humans. Infect Immun 1977; 18:130–7. [CrossRef] [PubMed]
Spear P, Manoj Sh, Yoon M, Jogger C, Zago A, Myscofski D. Different receptors binding to distinct interfaces on herpes simplex virus gD can trigger events leading to cell fusion and viral entry Virology 2006; 344(1):17-24 . [CrossRef] [PubMed]
Spruance SL, Evans TG, McKeough MB, et al. Th1/Th2-like immunity and resistance to herpes simplex labialis. Antiviral Res 1995; 28(1):39–55. [CrossRef] [PubMed]
Stamenkovic H, et al. Immune System Behavior during Herpesvirus Infection in Childhood. J Infect Dis Ther 2014; 2:5. [CrossRef]
Whitley R, Roizman B Herpes simplex viruses: is a vaccine tenable? J. Clin. Invest 2002; 109:145-51. [CrossRef] [PubMed]
