GUBITAK INTERSTICIJALNIH ĆELIJA KAHALA U TANKOM CREVU PACOVA SA DIJABETESOM MELITUSOM
Sažetak
Dijabetička gastroenteropatija predstavlja čestu komplikaciju dijabetesa melitusa. Poremećaj zastupljenosti intersticijalnih ćelija Kahala (IĆK) ima značajnu ulogu u patogenezi intestinalnih poremećaja motiliteta.
Cilj ove studije bio je da odredi distribuciju intramuskularnih i mijenteričnih IĆK u tankom crevu pacova sa dijabetesom melitusom.
Dijabetes melitus izazvan je streptozotocinom i nikotinamidom na animalnom modelu Wistar pacova muškog pola. Imunohistohemijsko ispitivanje vršeno je c-kit antitelom za identifikaciju IĆK. Zastupljenost intramuskularnih IĆK određivana je numeričkom arealnom gustinom, dok je za procenu mijenteričkih IĆK korišćen ICC-skor. Rezultati studije pokazali su smanjenje broja IĆK i njihovog umrežavanja u tankom crevu u grupi pacova sa dijabetesom.
Statistički značajan gubitak broja intramuskularnih i mijenteričnih IĆK zapažen je u svim uzorcima tankog creva pacova sa dijabetesom melitusom. Dijabetes značajno menja mikrookolinu IĆK i, najverovatnije, smanjenom signalizacijom preko insulina utiče na njihov opstanak.
Reference
Al-Shboul OA. The importance of interstitial cells of cajal in the gastrointestinal tract. Saudi J Gastroenterol 2013;19(1):3–15. [CrossRef] [PubMed]
Chang IY, Glasgow NJ, Takayama I, Horiguchi K, Sanders KM, Ward SM. Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction. J Physiol 2001;536(Pt 2):555–68. [CrossRef] [PubMed]
Connor JA, Kreulen D, Prosser CL, Weigel R. Interaction between longitudinal and circular muscle in intestine of cat. J Physiol 1977;273(3):665–89. [CrossRef] [PubMed]
den Braber-Ymker M, Heijker S, Lammens M, Nagtegaal ID. Practical and reproducible estimation of myenteric interstitial cells of Cajal in the bowel for diagnostic purposes. Neurogastroenterol Motil 2016;28(8):1261–7. [CrossRef] [PubMed]
Farrugia G. Interstitial cells of Cajal in health and disease. Neurogastroenterol Motil 2008;20(1):54–63. [CrossRef] [PubMed]
Forrest A, Huizinga JD, Wang X-Y, Liu LWC, Parsons M. Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus. Am J Physiol Gastrointest Liver Physiol 2008;294(1): G315-26. [CrossRef] [PubMed]
Fujishiro M, Kushiyama A, Yamazaki H, Kaneko S, Koketsu Y, Yamamotoya T, et al. Gastrointestinal symptom prevalence depends on disease duration and gastrointestinal region in type 2 diabetes mellitus. World J Gastroenterol 2017;23(36):6694–704. [CrossRef] [PubMed]
Horiguchi K, Semple GS, Sanders KM, Ward SM. Distribution of pacemaker function through the tunica muscularis of the canine gastric antrum. J Physiol 2001;537(Pt 1):237–50. [CrossRef] [PubMed]
Horváth VJ, Vittal H, Ordög T. Reduced insulin and IGF-I signaling, not hyperglycemia, underlies the diabetes-associated depletion of interstitial cells of Cajal in the murine stomach. Diabetes 2005;54(5):1528–33. [CrossRef] [PubMed]
Iino S, Horiguchi S, Horiguchi K, Hashimoto T. Interstitial cells of Cajal in Wsh/Wsh c-kit mutant mice. J Smooth Muscle Res 2020;56(0):58–68. [CrossRef] [PubMed]
Imaeda K, Takano H, Koshita M, Yamamoto Y, Joh T, Suzuki H. Electrical properties of colonic smooth muscle in spontaneously non-insulin-dependent diabetic rats. J Smooth Muscle Res 1998;34(1):1–11. [CrossRef] [PubMed]
Kashyap P, Farrugia G. Diabetic gastroparesis: what we have learned and had to unlearn in the past 5 years. Gut 2010;59(12):1716–26. [CrossRef] [PubMed]
Kito Y. The functional role of intramuscular interstitial cells of Cajal in the stomach. J Smooth Muscle Res 2011;47(2):47–53. [CrossRef] [PubMed]
Komuro T. Structure and organization of interstitial cells of Cajal in the gastrointestinal tract. J Physiol 2006;576(3):653-8. [CrossRef] [PubMed]
Lammers WJEP, Al-Bloushi HM, Al-Eisaei SA, Al-Dhaheri FA, Stephen B, John R, et al. Slow wave propagation and plasticity of interstitial cells of Cajal in the small intestine of diabetic rats. Exp Physiol 2011;96(10):1039–48. [CrossRef] [PubMed]
Lin L, Xu L, Zhang W, Ge Y, Tang Y, Zhang H, et al. Roles of stem cell factor on the depletion of interstitial cells of Cajal in the colon of diabetic mice. Am J Physiol Gastrointest Liver Physiol 2010;298(2): G241-7. [CrossRef] [PubMed]
Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, et al. Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes 1998;47(2):224–9. [CrossRef] [PubMed]
Mazet B, Raynier C. Interstitial cells of Cajal in the guinea pig gastric antrum: distribution and regional density. Cell Tissue Res 2004;316(1):23–34. [CrossRef] [PubMed]
Mei F, Han J, Huang Y, Jiang Z-Y, Xiong C-J, Zhou D-S. Plasticity of Interstitial Cells of Cajal: A Study in the Small Intestine of Adult Guinea Pigs. Anat Rec 2009;292(7):985–93. [CrossRef] [PubMed]
Ordög T, Hayashi Y, Gibbons SJ. Cellular pathogenesis of diabetic gastroenteropathy. Minerva Gastroenterol Dietol 2009;55(3):315–43. [PubMed]
Ordög T, Takayama I, Cheung WK, Ward SM, Sanders KM. Remodeling of networks of interstitial cells of Cajal in a murine model of diabetic gastroparesis. Diabetes 2000;49(10):1731–9. [CrossRef] [PubMed]
Rosa-e-Silva L, Troncon LE, Oliveira RB, Foss MC, Braga FJ, Gallo Júnior L. Rapid distal small bowel transit associated with sympathetic denervation in type I diabetes mellitus. Gut 1996;39(5):748–56. [CrossRef] [PubMed]
Sanders KM, Ward SM, Koh SD. Interstitial cells: regulators of smooth muscle function. Physiol Rev 2014;94(3):859–907. [CrossRef] [PubMed]
Sanders KM. Postjunctional electrical mechanisms of enteric neurotransmission. Gut 2000;47(4): iv23-5 ; discussion iv26. [CrossRef] [PubMed]
Seki K, Komuro T. Distribution of interstitial cells of Cajal and gap junction protein, Cx 43 in the stomach of wild-type and W/Wv mutant mice. Anat Embryol 2002;206(1–2):57–65. [CrossRef] [PubMed]
Sellin JH, Hart R. Glucose malabsorption associated with rapid intestinal transit. Am J Gastroenterol 1992;87(5):584–9. [PubMed]
Shimojima N, Nakaki T, Morikawa Y, Hoshino K, Ozaki H, Hori M, et al. Interstitial cells of Cajal in dysmotility in intestinal ischemia and reperfusion injury in rats. J Surg Res 2006;135(2):255–61. [CrossRef] [PubMed]
Suzuki M, Takahashi A, Toki F, Hatori R, Tomomasa T, Morikawa A, et al. The effects of intestinal ischemia on colonic motility in conscious rats. J Gastroenterol 2008;43(10):767–73. [CrossRef] [PubMed]
Suzuki T, Won K-J, Horiguchi K, Kinoshita K, Hori M, Torihashi S, et al. Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat. Am J Physiol Gastrointest Liver Physiol 2004;287(3): G638-46. [CrossRef] [PubMed]
Szkudelski T. Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp Biol Med 2012;237(5):481–90. [CrossRef] [PubMed]
Velickov A, Mitrovic O, Djordjevic B, Sokolovic D, Zivkovic V, Velickov A, et al. The effect of bilberries on diabetes-related alterations of interstitial cells of Cajal in the lower oesophageal sphincter in rats. Histol Histopathol 2017;32(6):639–47. [CrossRef] [PubMed]
Veličkov A, Radenković G, Petrović V VV. Diabetic alteration on Interstitial cells of Cajal. Acta Medica Median 2017;56(4):100–7. [CrossRef]
Veličkov AI, Djordjević B, Lazarević M, Veličkov A V, Petrović V, Jović M, et al. Distributions of Platelet-Derived Growth Factor Receptor-α Positive Cells and Interstitial Cells of Cajal in the Colon of Rats with Diabetes Mellitus Type 2. Medicina 2023;59(2):308. [CrossRef] [PubMed]
Vinik AI, Maser RE, Mitchell BD, Freeman R. Diabetic autonomic neuropathy. Diabetes Care 2003;26(5):1553–79. [CrossRef] [PubMed]
Wang X-Y, Huizinga JD, Diamond J, Liu LWC. Loss of intramuscular and submuscular interstitial cells of Cajal and associated enteric nerves is related to decreased gastric emptying in streptozotocin-induced diabetes. Neurogastroenterol Motil 2009;21(10):1095-e92. [CrossRef] [PubMed]
Ward SM, McLaren GJ, Sanders KM. Interstitial cells of Cajal in the deep muscular plexus mediate enteric motor neurotransmission in the mouse small intestine. J Physiol 2006; 573(1):147–59. [CrossRef] [PubMed]
Yamamoto T, Watabe K, Nakahara M, Ogiyama H, Kiyohara T, Tsutsui S, et al. Disturbed gastrointestinal motility and decreased interstitial cells of Cajal in diabetic db/db mice. J Gastroenterol Hepatol 2008;23(4):660–7. [CrossRef] [PubMed]
Yarandi SS, Srinivasan S. Diabetic gastrointestinal motility disorders and the role of enteric nervous system: current status and future directions. Neurogastroenterol Motil 2014;26(5):611–24. [CrossRef] [PubMed]
