REPLACING FISH MEAL WITH UNTREATED AND ENZYMATICALLY TREATED TORULA YEAST (CYBERLINDNERA JADINII) AFFECTS PELLETING DIE FLOW RESISTANCE AND PHYSICAL PROPERTIES OF THE FEED PELLETS
Abstract
Yeast is gaining importance as a novel feed ingredient. Although fishmeal generally provides better feed conversion in farmed aquatic animals, research suggests that the torula yeast Cyberlindnera jadinii (CJ) is a promising alternative to fishmeal. This study examines the effects of replacing fishmeal with torula yeast (CJ), both untreated and treated with protease and endo-exo 1.3-beta-glucanase, on pellet production. The first experiment evaluated changes in flow resistance and pellet quality when fishmeal was replaced with yeast. The second experiment focused on how enzyme-treated yeast influenced these factors. Pellets containing 20% CJ, whether treated or not, showed increased flow resistance and higher pellet strength. Pellets formulated with 10% and 20% CJ, as well as pellets composed solely of yeast material used as a control, exhibited water-repellent properties, potentially enhancing feed intake, reducing waste, and supporting sustainable production. However, pellets with 10% and 20% enzyme-treated CJ showed fat-repellent behavior, which may hinder post-production processes. Enzymatic treatment reduced underwater swelling in these pellets, and minimal hydrolysis is recommended to limit disintegration. Enzyme treatment reduced surface roughness, with pellets containing 20% treated CJ exhibiting the smoothest texture.
References
Agboola, J.O., Øverland, M., Skrede, A., & Hansen, J.Ø., (2020). Yeast as major protein‐rich ingredient in aquafeeds: A review of the implications for aquaculture production. Reviews in Aquaculture, 13(2), 949–970. https://doi.org/10.1111/raq.12507
Aslaksen, M. A., Kraugerud, O. F., Penn, M.,Svihus, B., Denstadli, V., Jorgensen, H. Y., Hillestad, M., Krogdahl, A., & Storebakken, T., (2007). Screening of nutrient digestibilities and intestinal pathologies in Atlantic salmon, Salmo salar, fed diets with legumes, oilseeds, or cereals. Aquaculture, 272 (1/4), 541-555. https://doi.org/10.1016/j.aquaculture.2007.07.22
Bansemer, M.S., Harris, J.O., Qin, J.G., Adams, L.R., Duong, D.N., & Stone, D.A.J., (2015). Growth and feed utilization of juvenile greenlip abalone (Haliotis laevigata) in response to water temperatures and increasing dietary protein levels. Aquaculture, 436, 13-20. https://doi.org/10.1016/j.aquaculture.2014.10.033
Feldmann, H. (2012). Yeast: molecular and cell biology (2nd ed.). Wiley-VCH Verlag GmbH & Co. KGaA.
Ferreira, N., Bonetti, C., & Seiffert, W. (2011). Hydrological and Water Quality Indices as management tools in marine shrimp culture. Aquaculture, 318, 425-433. https://doi.org/10.1016/j.aquaculture.2011.05.045
Ferreira, T. & Rasband, W. (2012). ImageJ User Guide: IJ 1.46r. National Institutes of Health.
Flemming, A.E. (1995). Growth, intake, feed conversion efficiency and chemosensory preference of the Australian abalone. Aquaculture, 132, 297-311.
Froehlich, H.E., Jacobsen, N.S., Essington, T.E., Clavelle, T., & Halpern, B.S., (2018). Avoiding the ecological limits of forage fish for fed aquaculture. Nature Sustainability, 1, 298–303. https://doi.org/10.1038/s41893-018-0077-1
Förch, R., Schönherr, H., & Jenkins, T.A. (Eds.) (2009). Contact angle goniometry (Appendix C). In R. Förch, H. Schönherr, & T.A. Jenkins (Eds.), Surface design: Applications in bioscience and nanotechnology (pp. 471-473). Wiley-VCH. https://doi.org/10.1002/9783527628599.app3
Gamboa-Delgado, J., Fernández-Díaz, B., Nieto-López, M., & Cruz-Suarez, L., (2015). Nutritional contribution of torula yeast and fish meal to the growth of shrimp Litopenaeus vannamei as indicated by natural nitrogen stable isotopes. Aquaculture. 453, (10), https://doi.org/10.1016/j.aquaculture.2015.11.026
Guo, J., Qiu, X., Salze, G., & Davis, D.A., (2019). Use of high-protein brewer’s yeast products in practical diets for the Pacific white shrimp Litopenaeus vannamei. Aquaculture Nutrition, 25, 680–690. https://doi.org/10.1111/anu.12889
Hermansson, A.M. (2000). Structure engineering. In Proceedings of the 2nd International Symposium on Food Rheology and Structure (pp. 47-56). Zurich, Switzerland.
Hetland, H., Svihus, B., & Olaisen, V. (2002). Effect of feeding whole cereals on performance, starch digestibility and duodenal particle size distribution in broiler chickens. British Poultry Science, 43(3), 416–423. https://doi.org/10.1080/00071660120103693
Hoseney, R.C., William, R.M., Lai, C.S., & Guetzlaff, J. (1992). Factors affecting the viscosity and structure of extrusion-cooked wheat starch. In Kokini, J.K., Ho, C.T., Karve, M.V. (Eds.), Food extrusion, science and technology (pp. 277-305). New York: Marcel Dekker
Huyben, D., Vidakovic, A., Nyman, A., Langeland, M., Lundh, T., & Kiessling, A. (2017). Effects of dietary yeasts and acute stress on blood parameters of dorsal aorta cannulated rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 43(2), 421-434. https://doi.org/10.1007/s10695-016-0297-0
Inborr, J., & Bedford, M.R. (1994). Stability of feed enzymes to steam pelleting during feed processing. Animal Feed Science and Technology, 46, (3–4), 179-196. https://doi.org/10.1016/0377-8401(94)90042-6
Jin, M., Xiong, J., Zhou, Q.-C., Yuan, Y., Wang, X.-X., & Sun, P. (2018). Dietary yeast hydrolysate and brewer’s yeast supplementation could enhance growth performance, innate immunity capacity and ammonia nitrogen stress resistance ability of Pacific white shrimp (Litopenaeus vannamei). Fish Shellfish Immunology, 82, 121–129. https:// doi.org/10.1016/j.fsi.2018.08.020
Klis, F.M., Mol, P., Hellingwerf, K., & Brul, S. (2002). Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiology Review, 26, 239–256. https://doi.org/10.1111/j.1574-6976.2002.tb00613.x
Korachkin, D., & Gethin, D.T. (2004). AEA Technology Ltd., An exploration of the effect of fill-density variation in the compaction of ferrous, ceramic and hard metal powder systems. AEAT/LD81000/05.
Langeland, M., Vidakovic, A., Vielma, J., Lindberg, J.E., Kiessling, A., & Lundh, T. (2014). Digestibility of microbial and mussel meal for Arctic charr (Salvelinus alpinus) and Eurasian perch (Perca fluviatilis). Aquaculture Nutrition, 22(2), 485–495. https://doi.org/10.1111/anu.12268
Li, S., Chen, W., Zongo, A. W-S., Chen, Y., Liang, H., Li, J., & Li, B. (2023). Effects of non-starch polysaccharide on starch gelatinization and digestibility: a review. Food Innovation and Advances, 2, 302–312., https://doi.org/10.48130/FIA-2023-0029
Li, Yu., Li, Yu., Wu, J., Deng, D., Meng, D., Sha, X., Liang, L., Zhang, Y., & Yang, R. (2025). Enzymatic hydrolysis enhances the stability of mannoprotein, stabilized O/W emulsion and the protective effect on β-carotene. Food and Bioprocess Technology, 18, 2632–2647. https://doi.org/10.1007/s11947-024-03619-2
Lovell, T. (1998). Nutrition and feeding of fish. New York: Springer Science+Business Media. https://doi.org/10.1007/978-1-4615-4909-3
Lowe, J.A., & Kershaw, S.J. (1996). Erratum to “Water activity-moisture content relationship as a predictive indicator for control of spoilage in commercial pet diet components”. Animal Feed Science Technology, 61, (1–4), 379-380. https://doi.org/10.1016/0377-8401(96)00971-6
Malcorps, W., Kok, B., van‘t Land, M., Fritz, M., van Doren, D., Servin, K., van der Heijden, P., Palmer, R., Auchterlonie, N.A., Rietkerk, M., Santos, M.J., & Davies, S.J., (2019). The sustainability conundrum of fishmeal substitution by plant ingredients in shrimp feeds. Sustainability, 11, 1212. https://doi.org/10.3390/su11041212
Miladinovic, D., Sørensen, M., & Svihus, B., (2013). Strength and durability of feed pellets influenced by different particle size distribution, pellet volume and dehydration techniques. Annual Transactions of the Rheology Society, 21, 107-115.
Miladinovic, D.D., Storebakken, T., Lekang, O.I., & Salas-Bringas, C., (2021). The effect of feed enzymes phytase, protease and xylanase on pelleting of microalgal biomass. Helyion, 7, (12), e08598. https://doi.org/10.1016/j.heliyon.2021.e08598
Mišljenović, N., Mosbye, J., Schüller, R.B., Lekang, O.I., & Salas-Bringas, C., (2015). Physical quality and surface hydration properties of wood based pellets blended with waste vegetable oil. Fuel Processing Technology, 134, 214-222. https://doi.org/10.1016/j.fuproc.2015.01.037
Obaldo, L.G., Divakaran, S., Tacon, A. G. (2002). Method for determining the physical stability of shrimp feeds in water. Aquaculture Research, 33, 369-377. https://doi.org/10.1046/j.1365-2109.2002.00681.x
Olvera-Novoa, M., Martinez-Palacios, C., & Olivera-Castillo, L., (2002). Utilization of torula yeast (Candida utilis) as a protein source in diets for tilapia (Oreochromis mossambicus Peters) fry. Aquaculture Nutrition, 8, 257-264. https://doi.org/10.1046/j.1365-2095.2002.00215.x
Oura, K., Katayama, M., Zotov, A. V., Lifshits, V. G., & Saranin, A. A. (2003). Elementary processes at surfaces I. Adsorption and desorption. In K. Oura, M. Katayama, A.V. Zotov, V.G. Lifshits & A.A.Saranin (Eds.), Surface Science: An Introduction (pp. 295-323). Berlin Heidelberg: Springer.
Pelletier, N., Klinger, D.H., Sims, N.A., Yoshioka, J.N., & Kittinger, J.N., (2018). Nutritional attributes, Substitutability, scalability, and environmental intensity of an illustrative subset of current and future protein sources for aquaculture feeds: Joint consideration of potential synergies and trade-offs. Environmental Science and Technology, 52, 5532–5544.
Roman-Gutierrez, A., Sabathier, J., Guilbert, S., Galet, L., & Cuq, B., (2003). Characterization of the surface hydration properties of wheat flours and flour components by the measurement of contact angle. Powder Technology, 129, 37-45. https://doi.org/10.1016/S0032-5910(02)00154-7
Salas-Bringas, C., Catargiu, A.M., Miladinovic, D., Schüller, R.B., & Mišljenović, N. (2015). Effects of enzymes and lignosulfonate addition on tensile strength, surface hydration properties and underwater swelling rate of microalgae pellets. Annual Transactions of the Nordic Rheology Society, 23, 153-160.
Salas-Bringas, C., Filbakk, T., Skjevrak, G., Lekang, O.I., Høibø, O., & Schüller, R.B. (2010). Assessment of a new laboratory die pelleting rig attached to a texture analyzer to predict processability of wood pellets. Energy consumption and pellet strength. Annual Transactions of the Nordic Rheology Society, 18, 77-86.
Salas-Bringas, C., Misljenovic, N., Wicklund, T., Lekang, O.I., & Schüller, R.B. (2011). Influence of particle size on strength of pelleted feed. Annual Transactions of the Nordic Rheology Society, 19, 293-301.
Sarkar, S., Ang, B.H., & Liew, C.V. (2014). Influence of starting material particle size on pellet surface roughness. American Association of Pharmaceutical Scientists, 15(1), 131-9. https://doi.org/10.1208/s12249-013-0031-5
Slade, L., & Levine, H. (1991). Beyond water activity: recent advances based on an alternative approach to the assessment of food quality and safety. Critical Reviews in Food Science and Nutrition, 30(2-3), 115-360. https://doi.org/10.1080/10408399109527543
Stokes, J.R., Boehm, M.W., & Baier, S.K. (2013). Oral processing, texture and mouthfeel: From rheology to tribology and beyond, Current Opinion in Colloid and Interface Science, 18, 349-359. https://doi.org/10.1016/j.cocis.2013.04.010
Saalah, S., Shapawi, R., Othman, N.A., & Bono, A. (2010). Effect of formula variation in the properties of fish feed pellet. Journal of Applied Sciences, 10, 2537-2543. https://doi.org/10.3923/jas.2010.2537.2543
Tacon, A.G.J., & Metian, M. (2015). Feed matters: satisfying the feed demand of aquaculture. Reviews in Fisheries Science & Aquaculture, 23, 1–10. https://doi.org/10.1016/j.cocis.2013.04.010
Tantikitti, C. (2014). Feed palatability and the alternative protein sources in shrimp feed. Songklanakarin Journal of Science and Technology, 36(1), 51-55.
Vidakovic, A., Huyben, D., Sundh, H, Nyman, A., Vielma, J., Passoth, V., Kiessling, A. & Lundh, T. (2020). Growth performance, nutrient digestibility and intestinal morphology of rainbow trout (Oncorhynchus mykiss) fed graded levels of the yeasts Saccharomyces cerevisiae and Wickerhamomyces anomalus. Aquaculture Nutrition, 26(2), 275-286. https://doi.org/10.1111/anu.12988
Vidakovic, A., Langeland, M., Sundh, H., Sundell, K., Olstorpe, M., Vielma, J., Kiessling, A. & Lundh, T. (2015). Evaluation of growth performance and intestinal barrier function in Arctic charr (Salvelinus alpinus) fed yeast (Saccharomyces cerevisiae), fungi (Rhizopus oryzae) and blue mussel (Mytilus edulis). Aquaculture Nutrition, 22(6), 1348–1360. https://10.1111/anu.12344
VKM. (2006). Assessment of the risk from Salmonella occurring in feedingstuffs and the feed production process (VKM Report 2006:20, pp. 32). Norwegian Scientific Committee for Food Safety (VKM), Panel on Biological Hazards, & Panel on Animal Health and Animal Welfare. https://vkm.no/download/18.d44969415d027c43cf1f4b3/1500303318525/4fefd597a8.pdf
Øverland, M., Karlsson, A., Mydland, L.T., Romarheim, O.H., & Skrede, A. (2013). Evaluation of Candida utilis, Kluyveromyces marxianus and Saccharomyces cerevisiae yeasts as protein sources in diets for Atlantic salmon (Salmo salar). Aquaculture, 402–403, 1-7. https://doi.org/10.1016/j.aquaculture.2013.03.016.
Copyright (c) 2026 The Authors

This work is licensed under a Creative Commons Attribution 4.0 International License.
