IN VITRO PROCENA ANTIOKSIDATIVNE AKTIVNOSTI EKSTRAKTA LATICA PAEONIA LACTIFLORA I CALENDULA OFFICINALIS UGRAĐENIH U NOVE FORME NOSAČA NA BIOBAZI
Sažetak
U ovom radu prikazana je ekstrakcija latica Paeonia lactiflora. i Calendula officinalis L. pomoću mikrotalasne reaktor i ultarazvika u sistemu etanol-voda i izolacija etarskog ulja latica Calendula officinalis L. hidrodestilacijom. Određen je ukupan sadržaj fenola i flavonoida u ekstraktima i ulju i procenjena je antioksidativna aktivnost. Antioksidativna aktivnost je određena korišćenjem standardnih antioksidativnih in vitro DPPH• i ABTS•+ testova. Najveći ukupan sadržaj fenola utvrđen je kod ekstrakata Paeonia lactiflora L. i Calendula officinalis L dobijenih ultrazvučnom ekstrakcijom (9,73 i 10,03 mg GA/mL RE, redom), dok je najveći sadržaj flavonoida dobijen ekstrakcijom uz pomoć mikrotalasnog reaktora (14,45 i 6,02 mg QE/mL RE, redom). Najveća antioksidativna aktivnost za ekstrakt Paeonia lactiflora dobijena je mikrotalasnom ekstrakcijom (79% i 83%), dok je za ekstrakt Calendula officinalis L. dobijena ultrazvučnom ekstrakcijom (45% i 49%) u DPPH• i ABTS •+, redom. Da bi se poboljšala antioksidativna aktivnost ekstrakta i etarskog ulja, pripremljeni su pektinski filmovi sa galnom kiselinom, uz pomoć enzimatski potpomognute katalize za postizanje kovalentnog vezivanja za nosač. Za karakterizaciju dobijenih filmova korišćena je optička mikroskopija i FTIR spektroskopija. Filmovi sa esencijalnim uljem Calendula officinalis L. i galnom kiselinom, pokazali su značajno povećan procenat inhibicije u DPPH• i ABTS•+ testu (91% i 95%, redom) nakon 10 minuta. Rezultati su, takođe, pokazali da se sve formulacije pektinskog biopolimernog filma, modifikovanog galnom kiselinom, mogu uspešno primenjivati kao nosač za oba tipa ingredijenata.
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