The Article COMPARISON OF LX-8000R AND URISED 2 FULL-AUTOMATED URINE ANALIZERS WITH MANUAL MICROSCOPIC EXAMINATION
Abstract
Background: Urinalysis has an important place in evaluating kidney and urinary tract infections. Automated urine analyzers enhance productivity and turnover in laboratories and economize time and labor required for analysis. In the present study, we evaluated and compared analytic and diagnostic performance of UriSed2 with LX-8000R, which is a novel image-based automated urine sediment analyzer.
Methods: A total of 178 urine samples sent to our laboratory were evaluated by the two urine analyzers and standard manual microscopy. Precision and comparison studies were done in accordance with CLSI guidelines.
Results: Sensitivity assessment revealed similar outcomes with both UriSed2 and LX-8000R devices for erythrocyte count (RBC), whereas UriSed2 device yielded higher outcomes for leukocyte count (WBC) and epithelial cells (EPI) than LX-8000R analyzer. Specificity of UriSed2 for WBC and RBC was higher than that of LX-8000R device. According to Gamma statistics, both urine analyzers showed perfect consistency for WBC, RBC and EPI cell counts. Manuel microscopy revealed statistically significant correlation between LX-8000R and UriSed2 in terms of WBC and RBC. Manual evaluation by Bland-Altman analysis demonstrated lower WBC and RBC values and higher EPI as compared to both UriSed2 and LX-8000R devices. As the result of Passing-Bablok regression analysis, both devices were found to be inconsistent with manual microscopy.
Conclusion: We think that evaluation of automated urine analyzers will be more meaningful when they are evaluated together with urine samples and patient clinic in addition to comparing with manual microscopy.
References
2. Mittal A, Sharma S.Comparison of Urised 2 Fully Automated Urine Analyzer to Manual Urine Mıcroscopy. Indıan Journal of Research May-2019; Volume-8, Issue-5. DOI: https://www.doi.org./10.36106/papirex
3. Yalcınkaya E, Erman H, Kırac E, Şerifoğlu A, Aksoy A, Isman FK, et al. Comparative Performance Analysis of Urised 3 and DIRUI FUS-200 Automated Urine Sediment Analyzers and Manual Microscopic Method. Medeniyet Med J. 2019; 34: 244-51. doi: 10.5222/MMJ.2019.23169
4. Huysal K, Üstündağ Y. Otomatik İdrar Analizörleri: Mikroskobik Bakı. Türk Klinik Biyokimya Derg 2015; 13(2): 83-87
5. Block DR, Lieske JC. Automated Urinalysis in the Clinical Lab. MLOMed Lab Obs 2012; 44: 8‐10, 12; quiz 14
6. Zaman Z, Fogazzi GB, Garigali G, Croci MD, Bayer G, Kránicz T. Urine sediment analysis: analytical and diagnostic performance of sedi-MAX® — A new automated microscopy image-based urine sediment analyser. Clin Chim Acta 2010; 411: 147‐154. doi:10.1016/j.cca.2009.10.018
7. http://www.longx.com.cn/en/index.asp
8. UriSed 2 Fully Automated Urine Sediment AnalyzerUser manual for SW version 2.0.4. 77 Elektronika Kft.
9. Mukakab MM. Statistics Corner: A Guide To Appropriate Use of Correlation Coefficient in Medical Research. Malawi Medical Journal 2012; 24 (3): 69–71.
10. Jintasuthanont P, Khejonnit V, Opaskiattikul N, Chinswangwatanakul W, Gonggetyai V. Evaluation of the Performance of the Automated Urine Sediment Analyzer “Urised” Compared with the Manual Method. Siriraj Med J 2010; 62: 152– 6.
11. Cho J, Oh KJ, Jeon BC, Lee SG, Kim JH. Comparison of Five Automated Urine Sediment Analyzers with Manual Microscopy for Accurate Identification of Urine Sediment. Clin Chem Lab Med 2019 Oct 25; 57 (11): 1744- 1753. DOI: https://doi.org/10.1515/cclm-2019-0211
12. Carlson DA, Statland BE. Automated urinalysis. Clin Lab Med 1988; 8: 449- 61.
13. Wesarachkitti B, Khejonnit V, Pratumvinit B, Reesukumal K, Meepanya S, Pattanavin C, et al. Performance Evaluation and Comparison of the Fully Automated Urinalysis Analyzers UX- 2000 and Cobas 6500. Lab Med 2016; 47 (2): 124– 33. DOI: 10.1093/labmed/lmw002
14. Zaman Z. Automated Urine Screening Devices Make Urine Sediment Microscopy in Diagnostic Laboratories Economically Viable. Clin Chem Lab Med 2015; 53:(Suppl 2: s1509- 11. DOI: 10.1515/cclm-2015-0476
15. Bottını PV, Martınez MHM, Garlıpp CR. Urinalysis: Comparison between Microscopic Analysis and a New Automated Microscopy Image-Based Urine Sediment Instrument. Clin Lab 2014; 60: 693- 697. DOI: 10.7754/Clin.Lab.2013.130725
16. Budak YU, Huysal K. Comparison of Three Automated Systems for Urine Chemistry and Sediment Analysis in Routine Laboratory Practice. Clin Lab 2011; 57 (1-2): 47– 52.
17. Ma J, Wang C, Yue J, Li M, Zhang H, Ma X, et al. Clinical Laboratory Urine Analysis: Comparison of the UriSed Automated Microscopic Analyzer and the Manual Microscopy. Clinical Laboratory, 01 Jan 2013; 59 (11-12): 1297-1303. DOI: 10.7754/clin.lab.2013.121128
18. Ercin U. A Comparative Study on the Performances of 77 Elektronika Urised 2 LabUmat2 and Dirui FUS200-H800 Urine Analyzers. Int J Med Biochem 2020; 3 (3): 171-7. DOI: 10.14744/ijmb.2020.04706
19. Akin OK, Serdar MA, Cizmeci Z, Genç Ö, Aydın S. Comparison of LabUMat with-UriSed and iQ®200 Fully Automatic Urine Sediment Analysers with Manual Urine Analysis. Biotechnol Appl Biochem 2009: 53: 139- 44. https://doi.org/10.1042/BA20080188
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