MiRNA-200b level in peripheral blood predicts renal interstitial injury in patients with diabetic nephropathy

The relationship between miRNA-200b level and DN

  • Tingfang Chen Department of Nephrology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
  • Zhenzhen Jiang
  • Haiying Zhang
  • Ruifeng Yang
  • Yan Wu
  • Yongping Guo
DOI: https://doi.org/10.5937/jomb0-40379
Keywords: MiRNA-200b, DN, Renal interstitial injury

Abstract


Background: To uncover the diagnostic potential of peripheral blood microRNA-200b (miRNA-200b) in renal interstitial injury in diabetic nephropathy (DN) patients.

Methods: A total of 50 diabetes subjects, 50 mild DN subjects, 50 moderate-severe DN subjects and 50 healthy subjects were included. Peripheral blood level of miRNA-200b in every subject was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Serum levels of renal function indicators were determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, relative levels of fibrosis damage indicators were examined by chemiluminescent immunoassay. Diagnostic potentials of miRNA-200b in diabetes, mild DN and moderate-severe DN were assessed by depicting receiver operating characteristic (ROC) curves.

Results: Peripheral blood level of miRNA-200b was higher in DN subjects than diabetes subjects without vascular complications, especially moderate-severe DN patients. Peripheral blood level of miRNA-200b in DN subjects was negatively correlated to relative levels of serum creatinine, urinary nitrogen, cystatin, TGF-β, CIV and PCIII. ROC curves demonstrated diagnostic potentials of miRNA-200b in mild and moderate-severe DN.

Conclusions: Peripheral blood level of miRNA-200b is closely linked to the degree of renal interstitial injury in DN patients. MiRNA-200b may be a vital indicator in predicting the development of DN.

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Published
2022/12/06
Issue
Vol. 42 No. 2 (2023): Journal of Medical Biochemistry
Section
Original paper

Copyright (c) 2022 Tingfang Chen, Zhenzhen Jiang, Haiying Zhang, Ruifeng Yang, Yan Wu, Yongping Guo

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