ASSESSMENT OF THE DIAGNOSTIC VALUE OF SERUM CATHEPSIN S AND ITS CORRELATION WITH HDL SUBCLASSES IN PATIENTS WITH NON-HODGKIN’S LYMPHOMA

Cathepsin S and HDL subclasses in lymphoma

  • Bosa Mirjanić-Azarić University Clinical Centre of the Republic of Srpska, Department of Clinical Biochemistry
  • Sinisa Stankovic University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina
  • Zana Radic Savic University of Banja Luka, Medical Faculty, Banja Luka, Republic of Srpska, Bosnia and Herzegovina
  • Dragana Malcic-Zanic University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina
  • Ana Ninic
  • Marija Vukovic University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina
  • Lana Nezic University of Banja Luka, Medical Faculty, Banja Luka, Republic of Srpska, Bosnia and Herzegovina
  • Ranko Skrbic University of Banja Luka, Medical Faculty, Banja Luka, Republic of Srpska, Bosnia and Herzegovina
  • Natasa Bogavac-Stanojevic University of Belgrade, Faculty of Pharmacy,Belgrade, Serbia
Keywords: cathepsin S, cystatin C, HDL3a and HDL3b subclasses, apoptosis, Hodgkin and non-Hodgkin lymphoma

Abstract


Background: Recent findings point to the key role of cathepsin S (CTSS) in the survival of malignant cells, as well as the significance of the anti-apoptotic properties of high-density lipoprotein (HDL) that contribute to enhanced cell survival.

The purpose of this study is to analyze CTSS as a potential biomarker in lymphoma. Also, in order to better understand the role of CTSS in the origin and development of lymphoma, its association with cystatin C (Cys C), lipids, and inflammatory markers was analyzed.

 Methods: The study included 90 subjects: 11 Hodgkin (HL) and 44 B-cell non-Hodgkin lymphoma (NHL) patients, and 35 healthy subjects. CTSS was determined using the Invitrogen ELISA kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Results: The level of CTSS was significantly higher in NHL patients than in control subjects: 12.20 (9.75-14.57) vs 9.97 (8.44-10.99), P<0.001. In NHL patients, there was a positive correlation between CTSS and the proportions of HDL3a, HDL3b, and the sum of the HDL3 subclasses (r=0.506, P<0.001; r=0.411, P=0.006, r=0.335, P=0.026, respectively). In addition, the area under the receiver operating characteristic curve (AUC curve) of CTSS was 0.766 (CI: 0.655-0.856).

Conclusions

CTSS is significantly elevated in patients with NHL and has the potential to be a new diagnostic biomarker. Moreover, demonstrating a correlation between CTSS levels and the proportion of anti-apoptotic HDL3a and HDL3b subclasses improves understanding of NHL, as well as contributes to the development of new therapeutic strategies for this cancer.

Published
2024/06/16
Section
Original paper