Diagnostic value and Clinical Significance of serum Proteins Ki-67, PI3K, and Fascin in Patients with Oral Squamous Cell Carcinoma
Clinical Significance of serum Proteins Ki-67, PI3K, and Fascin
Abstract
Objective: This study was designed to explore the expression and significance of KI-67, phosphoinositide-3-kinase (PI3K), and Fascin in oral squamous cell carcinoma (OSCC). Methods: Thirty-five patients with OSCC admitted to our hospital from January 2019 to December 2020 were included. Tumor tissue and paracancerous tissue samples of all patients were collected. Immunohistochemical staining was performed to determine the expression of Ki-67, PI3K, and Fascin. Pearson correlation analysis was employed to analyze the association of clinicopathological characteristics with Ki-67, PI3K, fascia, and oral cancer. SPSS 23.0 was used for data analysis. Results: Significantly higher positive expression rates of Ki-67, PI3K, and Fascin were detected in OSCC tissues, well-differentiated OSCC tissues, and OSCC tissues with lymph node metastasis and cancer cells infiltrating to peripheral muscle, vs paracancerous tissues, moderately and poorly differentiated OSCC tissues, and OSCC tissues without lymph node metastasis, respectively (all P<0.05). The positive expression rates of Ki-67, PI3K, and Fascin were not statistically different between stage I+II and III+IV OSCC (P>0.05). Ki-67 was positively correlated with PI3K (r=0.930, P=0.019) and Fascin (r=0.928, P=0.019), and Fascin was positively correlated with PI3K (r=0.991, P=0.001). Conclusion: The relative expression levels of Ki-67, PI3K, and Fascin are related to lymph node metastasis, perifocal muscle invasion, and pathological staging of patients with OSCC, which indicates their potential involvement in the occurrence and progression of OSCC.
References
[2] Lakshminarayana S, Augustine D, Rao RS, et al. Molecular pathways of oral cancer that predict prognosis and survival: A systematic review. J Carcinog. 2018 Dec 31;17:7. doi: 10.4103/jcar.JCar_17_18. PMID: 30766450; PMCID: PMC6334533.
[3] Cai Y, Dodhia S, Su GH. Dysregulations in the PI3K pathway and targeted therapies for head and neck squamous cell carcinoma. Oncotarget. 2017 Mar 28;8(13):22203-22217. doi: 10.18632/oncotarget.14729. PMID: 28108737; PMCID: PMC5400658.
[4] Szturz P, Vermorken JB. Management of recurrent and metastatic oral cavity cancer: Raising the bar a step higher. Oral Oncol. 2020 Feb;101:104492. doi: 10.1016/j.oraloncology.2019.104492. Epub 2019 Dec 11. PMID: 31837576.
[5] Lamb MC, Tootle TL. Fascin in Cell Migration: More Than an Actin Bundling Protein. Biology (Basel). 2020 Nov 17;9(11):403. doi: 10.3390/biology9110403. PMID: 33212856; PMCID: PMC7698196.
[6] Liu H, Zhang Y, Li L,et al. Fascin actin-bundling protein 1 in human cancer: promising biomarker or therapeutic target? Mol Ther Oncolytics. 2021 Jan 20;20:240-264. doi: 10.1016/j.omto.2020.12.014. PMID: 33614909; PMCID: PMC7873579.
[7] Pfisterer K, Jayo A, Parsons M. Control of nuclear organization by F-actin binding proteins. Nucleus. 2017 Mar 4;8(2):126-133. doi: 10.1080/19491034.2016.1267093.
[8] Almangush A, Heikkinen I, Mäkitie AA, et al. Prognostic biomarkers for oral tongue squamous cell carcinoma: a systematic review and meta-analysis. Br J Cancer. 2017 Sep 5;117(6):856-866. doi: 10.1038/bjc.2017.244. Epub 2017 Jul 27. PMID: 28751758; PMCID: PMC5589992.
[9] Kiyohara T, Miyamoto M, Shijimaya T,et al. Pseudovascular squamous cell carcinoma: A review of the published work and reassessment of prognosis. J Dermatol. 2018 Dec;45(12):1448-1451. doi: 10.1111/1346-8138.14640. Epub 2018 Sep 11. PMID: 30204258.
[10] Liu L, Xue X, Xue L. Liver metastatic basaloid squamous cell carcinoma with negative expression of pancytokeratin: a case report and literature review. Diagn Pathol. 2019 Sep 6;14(1):102. doi: 10.1186/s13000-019-0881-6. PMID: 31488173; PMCID: PMC6729002.
[11] Liu L, Xue X, Xue L. Liver metastatic basaloid squamous cell carcinoma with negative expression of pancytokeratin: a case report and literature review. Diagn Pathol. 2019 Sep 6;14(1):102. doi: 10.1186/s13000-019-0881-6. PMID: 31488173; PMCID: PMC6729002.
[12] Qiu X, Meng Y, Lu M, et al. Primary squamous cell carcinoma of the pancreas with a large pseudocyst of the pancreas as the first manifestation: a rare case report and literature review. BMC Gastroenterol. 2021 May 8;21(1):208. doi: 10.1186/s12876-021-01804-7. PMID: 33964875; PMCID: PMC8105924.
[13] Roslan NH, Makpol S, Mohd Yusof YA. A Review on Dietary Intervention in Obesity Associated Colon Cancer. Asian Pac J Cancer Prev. 2019 May 25;20(5):1309-1319. doi: 10.31557/APJCP.2019.20.5.1309. PMID: 31127882; PMCID: PMC6857900.
[14] Pirozzi F, Ren K, Murabito A, et al. PI3K Signaling in Chronic Obstructive Pulmonary Disease: Mechanisms, Targets, and Therapy. Curr Med Chem. 2019;26(16):2791-2800. doi: 10.2174/0929867325666180320120054. PMID: 29557740.
[15] Ijaz S, Akhtar N, Khan MS, et al. Plant derived anticancer agents: A green approach towards skin cancers. Biomed Pharmacother. 2018 Jul;103:1643-1651. doi: 10.1016/j.biopha.2018.04.113. Epub 2018 May 7. PMID: 29864953.
[16] Zhou Y, Hu W, Chen P, et al. Ki67 is a biological marker of malignant risk of gastrointestinal stromal tumors: A systematic review and meta-analysis. Medicine (Baltimore). 2017 Aug;96(34):e7911. doi: 10.1097/MD.0000000000007911. PMID: 28834915; PMCID: PMC5572037.
[17] Kiupel M, Camus M. Diagnosis and Prognosis of Canine Cutaneous Mast Cell Tumors. Vet Clin North Am Small Anim Pract. 2019 Sep;49(5):819-836. doi: 10.1016/j.cvsm.2019.04.002. Epub 2019 Jun 6. PMID: 31178200.
[18] Elmaci İ, Altinoz MA, Bolukbasi FH, et al. Paradoxical results obtained with Ki67-labeling and PHH3-mitosis index in glial tumors: a literature analysis. Clin Neuropathol. 2017 Nov/Dec;36(6):272-282. doi: 10.5414/NP301028. PMID: 28853695.
[19] Miettinen MM, Antonescu CR, Fletcher CDM, et al. Histopathologic evaluation of atypical neurofibromatous tumors and their transformation into malignant peripheral nerve sheath tumor in patients with neurofibromatosis 1-a consensus overview. Hum Pathol. 2017 Sep;67:1-10. doi: 10.1016/j.humpath.2017.05.010. Epub 2017 May 24. PMID: 28551330; PMCID: PMC5628119.
[20] Rindi G, Klimstra DS, Abedi-Ardekani B, et al. A common classification framework for neuroendocrine neoplasms: an International Agency for Research on Cancer (IARC) and World Health Organization (WHO) expert consensus proposal. Mod Pathol. 2018 Dec;31(12):1770-1786. doi: 10.1038/s41379-018-0110-y. Epub 2018 Aug 23. PMID: 30140036; PMCID: PMC6265262.
Copyright (c) 2025 Jianming Wei
This work is licensed under a Creative Commons Attribution 4.0 International License.
The published articles will be distributed under the Creative Commons Attribution 4.0 International License (CC BY). It is allowed to copy and redistribute the material in any medium or format, and remix, transform, and build upon it for any purpose, even commercially, as long as appropriate credit is given to the original author(s), a link to the license is provided and it is indicated if changes were made. Users are required to provide full bibliographic description of the original publication (authors, article title, journal title, volume, issue, pages), as well as its DOI code. In electronic publishing, users are also required to link the content with both the original article published in Journal of Medical Biochemistry and the licence used.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.