Role of Lipoprotein-associated phospholipase A2 (Lp-PLA2) in prediction and assessment of the severity of coronary artery disease in patients with type 2 Diabetes Mellitus Short title: Lp-PLA2 and coronary artery disease

Lp-PLA2 and coronary artery disease

  • Irena Kostovska Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia https://orcid.org/0000-0003-0971-6710
  • Svetlana Cekovska Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Katerina Tosheska Trajkovska Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Danica Labudovic Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Julijana Brezovska Kavrakova Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Sonja Topuzovska Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Hristina Ampova Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Melda Emin Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Elena Petrushevska Stanojevska Institute of Medical and Experimental Biochemistry, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Natasa Nedeska Minova 8–th September City General Hospital, Department of Endocrinology, Diabetes and Metabolic Diseases, Skopje, Republic of North Macedonia
  • Ognen Kostovski University Clinic of Digestive Surgery, Clinical Center “Mother Theresa”, 1000, Skopje, North Macedonia
  • Marjan Boshev University Clinic of Cardiology, Faculty of Medicine, Ss Cyril and Methodius University of Skopje, North Macedonia
DOI: https://doi.org/10.5937/jomb0-57486
Keywords: Lipoprotein-associated phospholipase A2, coronary artery disease, type 2 diabetes mellitus

Abstract


Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a specific biomarker associated with an increased risk of coronary artery disease (CAD) development. This study aims to determine the relationship between Lp-PLA2 activity and the risk of development and severity of CAD in patients with type 2 Diabetes mellitus (T2DM).

Methods: The cross-sectional study included 148 patients with T2DM, divided into two groups: patients with T2DM without confirmed CAD (n=56) and patients with T2DM and confirmed CAD (n=92), further divided into three subgroups based on the stage of CAD, and a control group of healthy individuals (n=44). Venous blood samples were collected from all participants to measure glucose, cholesterol, triglycerides, HDL, LDL, C-reactive protein, urea, and creatinine levels using standard photometric methods. Lp-PLA2 activity was measured using a chemiluminescent immunoassay method.

Results: Patients with T2DM and confirmed CAD had significantly higher Lp-PLA2 levels than those without confirmed CAD and healthy individuals. A significant difference in Lp-PLA2 levels was found between the group without CAD, the patients with CAD divided into subgroups according to disease stage, and the healthy control group. A positive correlation was observed between Lp-PLA2 and BMI, glycated hemoglobin, total cholesterol, and HDL cholesterol. The optimal cutoff value for Lp-PLA2 < 250 ng/ml yielded a diagnostic sensitivity of 95.65% and specificity of 88.64% for patients with T2DM and diagnosed CAD.

Conclusion: Lp-PLA2 can be used as a predictor for developing and assessing the severity of CAD in patients with T2DM.

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Published
2025/04/29
Issue
Vol. 44 No. 7 (2025): Journal of Medical Biochemistry
Section
Original paper

Copyright (c) 2025 Irena Kostovska, Svetlana Cekovska, Katerina Tosheska Trajkovska, Danica Labudovic, Julijana Brezovska Kavrakova, Sonja Topuzovska, Hristina Ampova, Melda Emin, Elena Petrushevska Stanojevska, Natasa Nedeska Minova, Ognen Kostovski, Marjan Boshev

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