Redoks disbalans u neutrofilima u akutnom koronarnom sindromu

  • Ilija M Dragojević Univerzitet u Prištini, Medicinski fakultet, Institut za Biohemiju
  • Dijana Mirić 1Medicinski fakultet, Univerzitet u Prištini- Kosovska Mitrovica, Kosovska Mitrovica, Republika Srbija
  • Bojana Kisić Medicinski fakultet, Univerzitet u Prištini- Kosovska Mitrovica, Kosovska Mitrovica, Republika Srbija
  • Dragana Puhalo Sladoje Medicinski fakultet u Foči, Univerzitet u Istočnom Sarajevu, Republika Srpska, BiH
  • Ljiljana Popović Medicinski fakultet, Univerzitet u Prištini- Kosovska Mitrovica, Kosovska Mitrovica, Republika Srbija
  • Dragiša Rašić Medicinski fakultet, Univerzitet u Prištini- Kosovska Mitrovica, Kosovska Mitrovica, Republika Srbija
DOI: https://doi.org/10.5937/jomb0-59659
Ključne reči: akutni koronarni sindrom , neutrofili, oksidativni stres

Sažetak


Kratak sadržaj

Uvod: Oksidativni stres ima ključnu ulogu u patogenezi akutnog koronarnog sindroma (AKS), a neutrofili doprinose oštećenju krvnih sudova preko produkcije reaktivnih kiseoničnih radikala i oksidanasa zavisnih od mijeloperoksidaze (MPO). Cilj istraživanja je bio da se  ispitaju promene markera oksidativnog stresa i aktivnosti antioksidativnih enzima u neutrofilima pacijenata sa AKS u poređenju sa kontrolnim subjektima.

Metod: Neutrofili su izolovani iz krvi 77 pacijenata sa AKS i 33 kontrolna subjekta. Oksidativni stres je procenjen merenjem konjugovanih diena, hidroperoksida i hloramina. Antioksidativni status je ocenjen određivanjem nivoa neproteinskih i ukupnih tiolnih grupa, kao i aktivnosti superoksid dismutaze (SOD), katalaze, glutation peroksidaze (GPx-1) i glutation reduktaze (GR). Takođe su određivane peroksidazna i hlorinišuća aktivnost MPO.

Rezultati: U grupi pacijenata sa AKS zabeležene su značajno više koncentracije konjugovanih diena (p < 0,005), hloramina (p < 0,005), tiolnih grupa (p < 0,05), aktivnosti SOD (p = 0,05) i hlorinišuće aktivnosti MPO (p < 0,05). Nisu nađene značajne razlike u aktivnostima katalaze, GPx-1, GR i peroksidazne aktivnosti MPO. Značajne korelacije uočene su između markera lipidne peroksidacije i antioksidativnih parametara, naročito SOD i MPO hlorinišuće aktivnosti. Višestruka regresiona analiza identifikovala je SOD i hloramine kao nezavisne prediktore lipidne peroksidacije.

Zaključak: Neutrofili pacijenata sa AKS pokazuju izražen redoks disbalans, karakterisan pojačanom lipidnom peroksidacijom, povećanim sadržajem tiola i povećanom hlorinišućom aktivnošću MPO. Ovi nalazi ukazuju na potencijal redoks parametara neutrofila kao biomarkera i terapijskih meta u AKS.

Ključne reči: akutni koronarni sindrom , neutrofili, oksidativni stress

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Objavljeno
2025/09/01
Broj časopisa
Ahead of print
Rubrika
Original paper

Sva prava zadržana (c) 2025 Ilija M Dragojevic, Dijana Miric, Bojana Kisic, Dragana Puhalo Sladoje, Ljiljana Popovic, Dragisa Rasic

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