Toxicity of Euphorbia helioscopia pellets to two phytophagous molluscs, Theba pisana Müller, 1774 (Pulmonata: Helicidae) and Arion hortensis Férussac, 1819 (Pulmonata: Arionidae)
Abstract
Harmful land snails and slugs are currently one of the most important threats facing agriculture in many parts of the world. Synthetic molluscicides are the main control method against these gastropods. However, dangers caused by these chemicals to the environment have led scientists to research for environmentally friendly alternatives. The objective of our work was to test and evaluate food pellets containing roots, stems, leaves or flowers of Euphorbia helioscopia against Theba pisana and Arion hortensis adults. Toxicity of the prepared pellets varied depending on plant organ and mollusc species tested. Pellets made of stems (LD50 = 1.35 g / 100 ml of agar at 2%) and leaves (LD50 = 1.39 g / 100 ml of 2% agar) proved more toxic to adult snails than those made of roots and flowers, which had no significant effects. In the case of slugs, pellets made of leaves (LD50 = 1.14 g / 100 ml of 2% agar) were more toxic than those made of stems (LD50 = 1.33 g / 100 ml of 2% agar), flowers (LD50 = 1.75 g / 100 ml of 2% agar) and roots (LD50 = 1.98 g / 100 ml of 2% agar). Compared to a synthetic product containing metaldehyde 5%, the results show that the use of these molluscicides derived from plants as pellets is environment- and healthconscious, targeted and economical. These products can be used in plant protection against phytophagous slugs and snails.
References
Abdallah, E.A.M., El-Wakil, H.B., Kassem, F.A., El-Agamy, E.I., & Bakr, Y.A. (1998). Impact of aldicarb and metaldehyde exposure on different molluscan enzyme activities and stress protein response. (7th Conference on Agricultre Ain Shams University, Cairo). Annals of Agricultural Science, 3, 1089 -1102.
Abdel-Hamid, A.Z. (1997). Development of bait formulations for control of intermediate hosts of African Schistosome species. Journal of Applied Toxicology, 17(6), 391-395. doi:10.1002/(sici)1099-1263(199711/12)17:6<391::aid-jat456>3.0.co;2-i
Abou el-Hasan, A.A., Soeb, A.H., Radwan, A.S., El-Eman, E.R., & Amin, S.M. (1980). The molluscicidal properties of Euphorbia lactea. In: Proceedings of 20th International Congress of Tropical Medicine and Malaria, Manilla (p 360).
Administration de l’hydraulique (1991). Ressources en eau dans le bassin de l’oued Beht (province de Khemisset). Publication de l’administration de l’hydraulique, ministere des travaux publics de la formation professionnelle et de la formation des cadres.
Al-Zanbagi, N.A. (2005). Two molluscicides from Saudi Arabian Euphorbiales against Bulinus wrighti. Journal of King Abdulaziz University-Science, 17, 11-19.
Al-Zanbagi N.A. (2013). Review of using plants as molluscicidal, larvicidal and schistosomicidal in Saudi Arabia. Australian Journal of Basic and Applied Sciences. 7(7),110-120.
Al-Zanbagi, N,A., Banaja A.A, & Barrett, J. (2000). Molluscicidal activity of some Saudi Arabian Euphorbiales against the snail Biomphalaria pfeifferi. Journal of Ethnopharmacology. 70(2), 119-125. doi:10.1016/s0378-8741(99)00155-5
Appleton, C.C. (1985). Molluscicides in bilharziasis control: The South African experience. South African Journal of Science, 81, 356-360.
Barker, G.M. (2002). Gastropods as pests in New Zealand pastoral agriculture, with emphasis on Agriolimacidae, Arionidae and Milacidae. In : G.M. Barker, Molluscs as crop pests (pp 361-424). Wallinford. UK: CABI.
Barla, A., Birman, H., Kultur, S., & Oksuz, S. (2006). Secondary metabolites from Euphorbia helioscopia and their vasodepressor activity. Turkish Journal of Chemistry, 30, 325-332.
Chambre d’agriculture (2016). Bulletin technique, agriculture biologique. Available at https://www.agrimaroc.net/bulletins/
Crowell, H.H. (1967). Slug and snail control with experimental poison baits. Journal of Economic Entomology, 60, 1048-1050.
Dai, J.R., Chen, C., Liang, Y.S., Zhang, Y.P., Zheng, Y.X., & Wu, F. (1998). Comparison among different samples of niclosamide ethanolamine salt wettable powder in molluscicidal effect and quality. Chinese Journal of Schistosomiasis Control, 10,86-88.
Eddaya, T., Boughdad, A., Zaid, A., & Amechrouq, A. (2010). Faune associee a la menthe dans la region de Meknes. In 7ème Congrès de l’AMPP (pp 135-142). Rabat, Morocco: AMPP.
El-Amin, S.M., & Osman, N.S. (1991). Squalene and Urs 12-en-28 01 from the Molluscicidal plant, Euphorbia helioscopia. Egyptian Journal Bilharzia, 13, 181-187.
El-Emam, M.A., El-Amin, S.M., & Shoeb, H.A. (1982). The molluscicidal properties of Euphorbiaceae, Euphorbia lactea. Helminthologia. 19, 227–236.
El-Zemity, S.R. (2006). Synthesis and molluscicidal activity of novel N-methyl carbamate derivatives based on naturally occurring monoterpenoides. Journal of Applied Sciences Research, 2, 86-90.
El-Zemity, S.R., & Radwan, M.A. (1999). Synthesis and molluscicidal properties of some (1H-1,2,4-Triazol-1-yl methyl) anilines, N-Alkylanilines and N,N-Diakylanilines. Journal of Pest Control and Environmental Science, 7, 89-102.
El-Zemity, S.R. & Radwan, M.A. (2001). Molluscicidal and antifeedant activity of some essential oils and their major chemical constituents against Theba pisana snails. Arab Universities Journal of Agricultural Sciences, 9,483-493.
Finney, D.J. (1971). Probit analysis. (3rd edition) (p 333). New York, NY: Cambridge University Press.
Garthwaite, D.G., & Thomas, M.R. (1996). The usage of molluscicides in agriculture and horticulture in Great Britain over the last 30 years. In: I.F. Henderson (ed.), Slug and Snail Pests in Agriculture, Proceedings of a symposium held in Canterbury, UK (pp 39-46). Thornton Heath, UK: British Crop Protection Council, Association of Applied Biologists and Malacological Society of London.
Gavin, W.E., Mueller-Warrant, G.W., Griffith, S.M., & Banowetz, G.M. (2012). Removal of molluscicidal bait pellets by earthworms and its impact on control of the gray field slug (Derocerus reticulatum Mueller) in western Oregon grass seed fields. Crop Protection, 42, 94-101. doi:10.1016/j.cropro.2012.05.023
Ghandour, A.M. (1991). Schistosomiasis in Saudi Arabia: Current status. CAB International Institute of Parasitology, 60, 1-13.
Glen, D.M., & Moens, R. (2002). Agriolimacidae, Arionidae and Milacidae as pests in West European cereals. In: G.M. Barker (Ed.), Molluscs as Crop Pests (pp 271-300). Wallingford, UK: CABI Publishing.
Hamdy, H.I. (2005). Composition of essential oils isolated from three plant species and their molluscicidal activity against Theba pisana snails. Journal of Pest Contol and Environmental Sciences, 13(2), 15-24.
Hamdy, H.I., & El-Wakil, H.B. (1996). A pioneer molluscicidal and antifeeding agent from Calotropis procera extract, against land snails. Journal of Pest Management Environmental, 1, 110-116.
Hamdy, H.I., Eshra, E.H., & Abu Bakr, A. (2007). Molluscicidal activity and biochemical effects of certain monoterpenoids against land snails. Journal of the Advances in Agricultural Research, 12(4), 679-693.
Hammond, R.B., & Byers, R.A. (2002). Agriolimacidae and Arionidae as pests in conservation-tillage soybean and maize cropping in North America (pp 301-314). In: G.M. Barker (Ed.), Molluscs as Crop Pests. Wallingford, UK: CABI.
Harmouzi, A., Boughdad, A., El Ammari, Y., & Chauch, A. (2016). Chemical composition and toxicity of Moroccan Tetraclinis articulata and Juniperus phoenicea essential oils against Aphis citricola Goot, 1912 (Homoptera, Aphididae). Research on Chemical Intermediates, 42(9), 7185-7197. Available at https://doi.org/10.1007/s11164-016-2528-5
Hommay, G.ET., & Briard, P. (1989). A few aspects of slug damage in France. In: Henderson, I.F. (Ed.), Slugs and Snails in World Agriculture (BCPC monograph 41) (pp 379-384). Thornton Heath, UK: British Crop Protection Council.
Howlett, S.A. (2012). Terrestrial slug problems: classical biological control and beyond. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 7(051). doi:10.1079/pavsnnr20127051
Iglesias, J., Castillejo, J., & Castro R. 2003. The effects of repeated applications of the molluscicide metaldehyde and the biocontrol nematode Phasmarhabditis hermaphrodita on molluscs, earth worms, nematodes, acarids and collembolans: a two-year study in northwest Spain, Pest Management Science, 59(11), 1217-1224. doi:10.1002/ps.758
Jenane, C., & Agbani, M. (2000). Fiche Technique : la betterave a sucre monogerme. Transfert de Technologie en Agriculture. Bulletin Mensuel d’Information et de Liaison du PNTTA, 75.
Kaplan, E.L., & Meier, P. (1958). Nonparametric estimation from incomplete observations. Journal of the American Statistical Association, 53(282), 457- 481. doi:10.1080/01621459.1958.10501452
Kinghorn, A.D., & Evans, F.J. (1975). A Biological screen of selected species of the genus Euphorbia for skin irritant effects. Planta Medica, 28(08), 325-335. doi:10.1055/s-0028-1097865
Kumar, P., & Singh, D.K. (2009). Use of amino acids and their combinations as attractant in bait formulations against the snail Lymnaea acuminata. Journal of Applied Bioscience, 35(1), 63-66.
Lakhili, F., Benabdelhadi, M., Bouderka, N., Lahrach, H., & Lahrach A. (2015). Etude de la qualite physicochimique et de la contamination metallique des eaux de surface du bassin versant de Beht (Maroc). European Scientific Journal, 11(4), 1857-7431.
Liu, S.Y., Sporer, F., Wink, M., Jourdanes, J., Henning, R., Li, Y.L., & Ruppel, A. (1997). Anthraquinones in Rheum palmatum and Rumex dentatus (Polygonaceae) and phorbol esters in Jatropha curcas (Euphorbiaceae) with molluscicidal activity against the schistosome vector snails Oncomelania, Biomphalaria and Bulinus. Tropical Medicine & International Health. 2(2), 188-189. doi:10.1046/j.1365-3156.1997.d01-242.x
Marston, A., & Hostettmann, K. (1985). Plant molluscicides. Phytochemistry, 24(4), 639-652. doi:10.1016/s0031-9422(00)84870-0
McCullough, F.S., Gayral, P.H., Duncan, J., & Christie, J.D. (1981). Les molluscicides dans la lutte contre la schistosomiase. Bulletin de l’Organisation mondiale de la Sante, 59(1), 17-26.
Mendes, N.M., Vasconcellos, M.C., Baptista, D.F., Rocha, R.S., & Schall, V.T. (1997). Evaluation of the molluscicidal properties of Euphorbia splendens var. hislopii (N.E. B.) latex: experimental test in an endemic area in the state of Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 92(5), 719-724. doi:10.1590/s0074-02761997000500029
Meredith, R.H. (2003). Slug pellets – risks and benefits in perspective. In: G.B.J. Dussart (ed.), Slug and snails: agricultural, veterinary and environmental perspectives (British Crop Protection Conference, Symposium Proceedings No. 88, Canterbury, UK, pp 235–242). Aldershot, UK: BCPC.
Oliveira-Filho, E.C., & Paumgartten, F.J.R. (2000). Toxicology of Euphorbia milii latex and niclosamide to snails and nontarget aquatic species. Ecotoxicology and Environmental Safety, 46(3), 342-350.
Port, G., & Ester, A. (2002). Gastropods as pests in vegetable and ornamental crops in Western Europe. In: G.M. Barker (Ed.). Molluscs as crop pests (pp 337-351). Wallingford, UK: CABI Publishing.
Radwan, M.A., & El-Zemity, S.R. (2007). Naturally occurring compounds for control of harmful snails. Pakistan Journal of Zoology, 39(5), 339-344.
Redbond, M. (2003). Slugs and snails: Pesticide Outlook, 14(5), 213. doi:10.1039/b311462g
Rungs, C.E.E. (1962). La faune nuisible a la betterave. Al Awamia, 3, 161-174.
Shoeb, H.A., & El-Sayed, M.M. (1984). A short communication on the molluscicidal properties of some plants from Euphorbiaceae and Agavaceae. Helminthologia, 21, 33-54.
Singh, A., & Agrawal, R.A. (1990). Molluscicidal and anticholinesterase activity of Euphorbiaceae. Biological Agriculture and Horticulture, 7(1), 81-91. doi:10.1080/01448765.1990.11978497
Singh, A., & Agrawal, R.A. (1992). Molluscicidal activity of Euphorbiales against the snail Indoplanorbis exustus. Acta Hydrochimica et Hydrobiologica, 20(5), 262–264. doi:10.1002/aheh.19920200502
Singh, P., & Singh, D.K. (2008). Binary combination of carbohydrates and amino acids as snail attractant in pellets containing molluscicides against the snail Lymnaea acuminate. Pesticide Biochemistry and Physiology. 92(3), 120-124. doi:10.1016/j.pestbp.2008.07.002
Singh, S.K., Yadav, R.P., & Singh, A. (2009). Molluscicides from some common medicinal plants of eastern Uttar Pradesh, India. Journal of Applied Toxicology, 30(1), 1-7. doi:10.1002/jat.1498
South, A. (1992). Terrestrial slugs: Biology, ecology, and control. London, UK: Chapman and Hall.
Steinmann, V.W., & Porter, J.M. (2002). Phylogenetic relationships in Euphorbieae (Euphorbiaceae) based on its and ndhF sequence data. Annals of the Missouri Botanical Garden, 89(4),453-490. doi:10.2307/3298591
Strufe R. (1968). Problems and results of residue studies after application of molluscicides. Residue Reviews, 24, 79-168.
Tadros, T.F. (1980). Control and assessment of the physical stability of pesticide suspension concentrates. Society of Chemistry and Industry, 18, 211-218.
Tanji, A. (2008). Conduite tehnique de la menthe: Diagnostic dans la province de Settat. Bulletin mensuel d’ information et de liaison du PNTTA, transfet de technomogie en agronomie, N167. Available at www. agrimaroc.net/2018/05/15/conduite-technique-dela-menthe-diagnostic-dans-la-province-de-settat/8/.
Tiwari, F. (2012). Bait formulation toxicity of plant derived molluscicides in attractant food pellets against vector snail, Lymnaea acuminate. World Journal of Zoology. 7 (1), 55-59.
Tiwari, F., & Singh, D.K. (2004a). Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species, Brazilian Journal of Medical and Biological Research, 37(4), 587-590. doi:10.1590/s0100-879x2004000400016
Tiwari, F., & Singh, D.K. (2004b). Behavioural responses of the snail Lymnaea acuminata to carbohydrates in snailattractant pellets. Naturwissenschaften. 91(8), 378-380. doi:10.1007/s00114-004-0538-4
Tiwari, F., & Singh, D.K. (2007). Toxicity test of plant derived molluscicides with attractant food pellets against snail, Lymnaea acuminata. Iranian Journal of Pharmacology and Therapeutics, 6, 103-107.
Uzair, M., Loothar, B.A., & Choudhary, B.A. (2009). Biological screening of Euphorbia helioscopia, L. Pakistan Journal of Pharmaceutical Sciences, 22(2), 184-186.
Zani, C.L., Marston, A., Hamburger, M., & Hostettmann, K. (1993). Chemistry of Brazilian Euphorbiaceae 1. Molluscicidal milliamines from Euphorbia milii var. hislopii. Phytochemistry. 34(1), 89-95. doi:10.1016/s0031-9422(00)90788-x
Zhang, T. & Jiang Q.W. (2002). Study on toxicology of niclosamide. Chinese Journal of Schistosomiasis Control, 14(3), 234-236.
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