Impact of raspberry leaf blotch emaravirus on red raspberry ‘Willamette’ fruits
Abstract
Raspberry leaf blotch emaravirus (RLBV) has become established in many Serbian raspberry orchards as the most prevalent virus of raspberries in the country. The aim of this study was to evaluate the impact of RLBV on the red raspberry ‘Willamette’ variety. A trial was conducted in four raspberry orchards located in Western Serbia. Fruits from RLBVinfected and uninfected canes were analyzed for fruit size (fruit length, width, height, shape, and weight), soluble solids content, pH, titratable acidity, total sugars, and total phenolic and anthocyanin contents. The results of the study confirmed that RLBV significantly decreases fruit size and weight (9.15-27.49%) of ‘Willamette’ fruits. Soluble solids content was higher in infected fruits (1.55-7.39%), but the increase was not significant. RLBV did not cause significant changes in titratable acidity of raspberry juice, pH or total sugars content. Total phenolic and anthocyanin contents were higher in fruits of RLBV-infected plants in two out of four locations.
References
Bi, Y., Artola, K., Kurokura, T., Hytönen, T., & Valkonen, J.P.T. (2012). First report of raspberry leaf blotch virus in raspberries in Finland. Plant Disease, 96, 1231. doi: 10.1094/PDIS-04-12-0368-PDN
Delić, D., Radulović, M., Vakić, M., Sunulahpašić, A., Villamor, D.E.V., & Tzanetakis, I.E. (2020). Raspberry leaf blotch emaravirus in Bosnia and Herzegovina: population structure and systemic movement. Molecular Biology Reports, 47(6), 4891-4896. doi: 10.1007/s11033-020-05560-x
Dobrivojević, K., & Petanović, R. (1985). Eriophyd raspberry leaf mite, Phyllocoptes gracilis (Nal.)(Eriophyoidea, Acarina), an insufficiently known pest in Yugoslavia. Zaštita bilja, 36, 254-260.
Dong, L., Lemmetty, A., Latvala, S., Samuilova, O., & Valkonen, J.P.T. (2016). Occurrence and genetic diversity of Raspberry leaf blotch virus (RLBV) infecting cultivated and wild Rubus species in Finland. Annals of Applied Biology, 168, 122-132. doi: 10.1111/aab.12247
Elbeaino, T., Digiaro, M., Mielke-Ehret, N., Muehlbach, H.P., & Martelli, G.P. (2018). ICTV virus taxonomy profile: Fimoviridae. Journal of General Virology, 99, 1478-1479. doi: 10.1099/jgv.0.001143
Howard, L.R., Clark, J.R., & Brownmiller, C. (2003). Antioxidant capacity and phenolic content in blueberries as affected by genotype and growing season. Journal of the Science of Food and Agriculture, 83(12), 1238-1247. doi: 10.1002/jsfa.1532
Jevremović, D., Leposavić, A., & Paunović, A.S. (2016). Raspberry leaf blotch virus – a common raspberry pathogen in Serbia. Journal of Mountain Agriculture on the Balkans, 19(3), 147-156.
Jevremović, D., Leposavić, A., & Paunović A.S. (2019). Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia. Spanish Journal of Agricultural Research, 17(1), e1004. doi: 10.5424/sjar/2019171-13861
Kalt, W., Ryan, D.A.J., Duy, J.C., Prior, R.L., Ehlenfeldt, M.K., & Vander Kloet, S.P. (2001). Interspecific variation in anthocyanins, phenolics, and antioxidant capacity among genotypes of highbush and lowbush blueberries (Vaccinium Section cyanococcus spp.) Journal of Agricultural and Food Chemistry, 49(10), 4761-4767. doi: 10.1021/jf010653e
Liu, M., Li, X.Q., Weber, C., Lee, C.Y., Brown, J., & Liu, R.H. (2002). Antioxidant and antiproliferative activities of raspberries. Journal of Agricultural and Food Chemistry, 50(10), 2926-2930. doi: 10.1021/jf0111209
Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J., & Kinard, G. (2008). A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods, 154, 48-55. doi: 10.1016/j.jviromet.2008.09.008
McGavin, W.J., Mitchell, C., Cock, P.J., Wright, K.M., & MacFarlane, S.A. (2012). Raspberry leaf blotch virus, a putative new member of the genus Emaravirus, encodes a novel genomic RNA. Journal of General Virology, 93, 430-437. doi: 10.1099/vir.0.037937-0
Milenković, S., & Marčić, D. (2011). Raspberry leaf and bud mite (Phyllocoptes gracilis) in Serbia: the pest status and control options. Acta Horticulturae, 946, 253-256. doi: 10.17660/ActaHortic.2012.946.40
Petrović, S., Leposavić, A., & Jevremović, D. (2017). Raspberry - The management, processing and marketing. Čačak, Serbia: Scientific Pomological Society of Serbia.
Pozhylov, I., Snihur, H., & Budzanivska, I. (2021). Phylogenetic analysis of Ukrainian isolate of raspberry leaf blotch virus. Agrofor International Journal, 6(1), 19-25. doi: 10.7251/AGRENG2101019P
Singleton, V.L., Orthofer, R., & Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178. doi: 10.1016/S0076-6879(99)99017-1
Tanner, H., & Brunner, H.R. (1979). Getränke-Analytik: Untersuchungsmethoden für die Labor- und Betriebspraxis. Schwäbisch Hall, Germany: Verlag Heller Chemie-und Verwaltungsgesellschaft.
Authors retain copyright of the published papers and grant to the publisher the non-exclusive right to publish the article, to be cited as its original publisher in case of reuse, and to distribute it in all forms and media.
The published articles will be distributed under the Creative Commons Attribution ShareAlike 4.0 International license (CC BY-SA). It is allowed to copy and redistribute the material in any medium or format, and remix, transform, and build upon it for any purpose, even commercially, as long as appropriate credit is given to the original author(s), a link to the license is provided, it is indicated if changes were made and the new work is distributed under the same license as the original.
Users are required to provide full bibliographic description of the original publication (authors, article title, journal title, volume, issue, pages), as well as its DOI code. In electronic publishing, users are also required to link the content with both the original article published in Pesticidi i fitomedicina (Pesticides and Phytomedicine) and the licence used.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.