Antioxidant activity of Juglans regia L. and Rumex obtusifolius L. leaf extracts and screening for their allelopathic potential
Abstract
Secondary plant metabolites with allelopathic activity or phytotoxicity could be biotechnologically important, serving as a source of allelochemicals, and thus contributing to the agro-industrial sector. The objective of this study was to use the obtained common walnut (Juglans regia L.) and bitter dock (Rumex obtusifolius L.) leaves extracts rich in phenolic compounds, i.e. with high antioxidant potential, and to identify their phytotoxicity to Setaria glauca (L.) P. Beauv. and Sorghum halepense (L.) Pers. weed seedlings grown in vitro. The obtained plant extracts had remarkably high affinity for scavenging free radicals, having DPPH IC50 values of 0.127 mg/ml for common walnut leaf extract and 0.194 mg/ml for bitter dock leaf extract. Ferric reducing antioxidant power of the extracts was also high, FRAP value of the common walnut leaf extract
was 384.4 ± 8.1 μmol Fe2+/g dry mass, and of the bitter dock leaf extract 321.6 ± 2.5 μmol Fe2+/g dry mass. At the highest used concentration, common walnut leaf extract reduced germination of S. glauca by 67.3%, while bitter dock leaf extract reduced germination of that weed by 54.5%. Shoot length of S. glauca was inhibited 80.7% when subjected to common walnut leaf extract, and 78.2% under the influence of bitter dock leaf extract, and its root length was inhibited 96.4% and 93.1% respectively. Germination of S. halepense was inhibited 100% under the influence of the obtained common walnut leaf extract at its highest test concentration, and 79.2% when subjected to bitter dock leaf extract at the same concentration. Shoot length of this weed was reduced 100% after treatment with common walnut leaf extract, and 93.7% when subjected
to bitter dock leaf extract. Root length was reduced 100% and 99.3%, respectively. Overall, the extracts demonstrated pronounced antioxidant activity and remarkable allelopathic potential.
References
Amaral, J. S., Valentao, P., Andrade, P. B., Martins, R. C., & Seabra, R. M. (2008). Do cultivar, geographical location and crop season influence phenolic profile of walnut leaves? Molecules, 13, 1321-1332. doi:10.3390/molecules13061321
Arroyo, A.I., Pueyo, Y., Pellissier, F., Ramos, J., Espinosa-Ruiz, A., Millery, A., & Alados, C.L. (2018). Phytotoxic effects of volatile and water-soluble chemicals of Artemisia herba-alba. Journal of Arid Environments, 151, 1-8. doi:10.1016/j.jaridenv.2017.11.010
Babula, P., Vaverkova, V., Poborilova, Z., Ballova, L., Masarik, M., & Provaznik, I. (2014). Phytotoxic action of naphthoquinone juglone demonstrated on lettuce seedling roots. Plant Physiology and Biochemistry, 84, 78-86. doi: 10.1016/j.plaphy.2014.08.027
Camen, D., Dragomir, N., Horablaga, M., Dragomir, C., Rechiţean, D., & Dragoş, M. (2017). Allelopathic aspects in Rumex crispus L. and Rumex obtusifolius L. II. Allelopathic effect on grassland legumes. Romanian Journal of Grassland and Forage Crops, 15, 19-24.
Carvalho, M., Ferreira, P.J., Mendes, V.S., Silva, R., Pereira, J.A., Jerónimo, C., & Silva, B.M. (2010). Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food and Chemical Toxicology, 48, 441-447. doi: 10.1016/j.fct.2009.10.043
Chelly, M., Chelly, S., Occhiuto, C., Cimino, F., Cristani, M., Saija, A. … Siracusac, L. (2021). Comparison of phytochemical profile and bioproperties of methanolic extracts from different parts of tunisian Rumex roseus. Chemistry and Biodiversity, 18, e2100185. doi: 10.1002/cbdv.202100185
Dragomir, N., Horablaga, M., Camen, D., Dragomir, C., Rechiţean, D., & Dragoş, M. (2017). Allelopathic aspects in Rumex crispus L. and Rumex obtusifolius L. I. Allelopathic effect on grassland grasses. Romanian Journal of Grassland and Forage Crops, 15, 31-37.
Ercisli, S., Esitken, A., Turkkal, C., & Orhan, E. (2005). The allelopathic effects of juglone and walnut leaf extracts on yield, growth, chemical and PNE compositions of strawberry cv. Fern. Plant, Soil and Environment, 51(6), 283–287. doi: 10.17221/3587-PSE
Ercisli, S., & Turkkal, C. (2005). Allelopathic effects of juglone and walnut leaf extracts on growth, fruit yield and plant tissue composition in strawberry cvs. ‘Camarosa’ and ‘Sweet Charlie’. Journal of Horticultural Science and Biotechnology, 80, 39-42. doi: 10.1080/14620316.2005.11511888
Franco, D.M., Silva, E.M., Saldanha, L.L., Adachi, S.A., Schley, T.R., Rodrigues, T.M. … Rolim de Almeida, L.F. (2015). Flavonoids modify root growth and modulate expression of short-root and HD-ZIP III. Journal of Plant Physiology, 188, 89-95. doi: 10.1016/j.jplph.2015.09.009
Gaaliche, B., Ladhari, A., Medeiros, A.G., Ben Mimoun, M., & Hajlaoui, M.R. (2017). Relationship between phytochemical profiles and phytotoxic proprieties of Tunisian fig leaf cultivars. South African Journal of Botany, 112, 322-328. doi: 10.1016/j.sajb.2017.06.015Get
Gnanavel, I ., & Natarajan, S. K. (2014). Ecofriendly weed control options for sustainable agriculture. Agricultural Reviews, 35(3), 172. doi: 10.5958/0976-0741.2014.00904.0
Harshaw, D., Nahar, L., Vadla, B., Saif-e-Naser, G. M., & Sarker, S.D. (2010). Bioactivity of Rumex obtusifolius (Polygonaceae). Archives of Biological Science, 62(2), 387-392. doi: 10.2298/ABS1002387H
Hazrati, H., Saharkhiz, M. J., Moein, M., & Khoshghalb, H. (2018). Phytotoxic effects of several essential oils on two weed species and tomato. Biocatalysis and Agricultural Biotechnology, 13, 204-212. doi: 10.1016/j.bcab.2017.12.014
Hossen, K., Ozaki, K., Teruya, T., & Kato-Noguchi, H. (2021). Three active phytotoxic compounds from the leaves of Albizia richardiana (Voigt.) King and Prain for the development of bioherbicides to control weeds. Cells, 10, 2385. doi: 10.3390/cells10092385
Jabran, K., & Farooq, M. (2013). Implications of potential allelopathic crops in agricultural systems. In Cheema, Z., Farooq, M., Wahid, A. (eds), Allelopathy (pp. 349-385). Berlin/Heidelberg, Germany: Springer. doi: 10.1007/978-3-642-30595-5_15
Jimoh, F.O., Adedapo, A.A., Aliero, A.A., &. Afolayan, A.J (2008) Polyphenolic contents and biological activities of Rumex ecklonianus. Pharmaceutical Biology, 46(5), 333-340. doi: 10.1080/13880200801887765
Kaur, S., Kaur, R., & Chauhan, B.S. (2018). Understanding crop-weed-fertilizer-water interactions and their implications for weed management in agricultural systems. Crop Protection, 103, 65-72. doi: 10.1016/j.cropro.2017.09.011
Kocacë Aliskan, I., & Terzi, I. (2001). Allelopathic effects of walnut leaf extracts and juglone on seed germination and seedling growth. Journal of Horticultural Science and Biotechnology, 76, 436–440. doi: 10.1080/14620316.2001.11511390
Levizou, E.F.I., Karageorgou, P., Psaras, G.K., & Manetas, Y. (2002). Inhibitory effects of water-soluble leaf leachates from Dittrichia viscosa on lettuce root growth, statocyte development and graviperception. Flora: Morphology, Distribution, Functional Ecology of Plants, 197, 152-157. doi: 10.1078/0367-2530-00025
Litvinenko, Y.A., & Muzychina, R. A. (2008). New antioxidant phytopreparation from Rumex thyrsiflorus roots. III. Chemistry of Natural Compounds, 44, 239-240. doi: 10.1007/s10600-008-9026-y
Li, Z.H., Wang, Q., Ruan, X., Pan, C.D., & Jiang, D.A. (2010). Phenolics and plant allelopathy. Molecules, 15(12), 8933-8952. doi: 10.3390/molecules15128933
Medic, A., Zamljen, T., Slatnar, A., Hudina, M., & Veberic, R. (2021). Is juglone the only naphthoquinone in Juglans regia L. with allelopathic effects? Agriculture, 11, 784. doi: 10.3390/agriculture11080784
Mominul Islam, A.K.M., & Kato-Noguchi, H. (2013). Plant growth inhibitory activity of medicinal plant Hyptis suaveolens: Could allelopathy be a cause? Emirates Journal of Food and Agriculture, 25, 692-701. doi: 10.9755/ejfa.v25i9.16073
Mominul Islam, A.K.M., & Kato-Noguchi H. (2014). Phytotoxic activity of Ocimum tenuiflorum extracts on germination and seedling growth of different plant species. Scientific World Journal, 2014, 1–8. doi: 10.1155/2014/676242
Morikawa, C.I.O., Miyaura, R., de Lourdes Tapia y Figueroa, M., Liliana, E., Rengifo Salgado, E.L., & Fujii, Y. (2012). Screening of 170 Peruvian plant species for allelopathic activity by using the sandwich method. Weed Biology and Management, 12, 1–11. doi: 10.1111/j.1445-6664.2011.00429.x
Muscolo, A., Panuccio, M.R., & Sidari, M. (2001). The effect of phenols on respiratory enzymes in seed germination. Plant Growth Regulation, 35, 31-35. doi: 10.1023/A:1013897321852
Nishida, N., Tamotsu, S., Nagata, N., Saito, C., & Sakai, A. (2005). Allelopathic effects of volatile monoterpenoids produced by Salvia leucophylla: Inhibition of cell proliferation and DNA synthesis in the root apical meristem of Brassica campestris seedlings. Journal of Chemical Ecology, 31, 1187-1203. doi: 10.1007/s10886-005-4256-y
Olofsdotter, M. (2001). Rice – A step toward use of allelopathy. Agronomy Journal, 93, 3-8. doi: 10.2134/agronj2001.9313
Pereira, J.A., Oliveira, I., Sousa, A., Valentao, P., Andrade, P.B., Ferreira, I.C.F.R. … Estevinho, L. (2007). Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food and Chemical Toxicology, 45(11), 2287-2295. doi: 10.1016/j.fct.2007.06.004
Piyatida, P., & Kato-Noguchi, H. (2010). Screening of allelopathic activity of eleven Thai medicinal plants on seedling growth of five test plant species. Asian Journal of Plant Sciences, 9, 486-491. doi: 10.3923/ajps.2010.486.491
Prior, R.L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290-4302. doi: 10.1021/jf0502698
Qian, H., Xu, J., Lu, T., Zhang, Q., Qu, Q., Yang, Z., & Pan, X. (2018). Responses of unicellular alga Chlorella pyrenoidosa to allelochemical linoleic acid. Science of the Total Environment, 625, 1415-1422. doi: 10.1016/j.scitotenv.2018.01.053
Rice, E.L. (1984). Allelopathy, 2nd ed. Orlando, FL, USA: Academic Press.
Rob, M.M, Hossen, K., Khatun, M.R., Iwasaki, K., Iwasaki, A., Suenaga, K., & Kato-Noguchi, H. (2021). Identification and application of bioactive compounds from Garcinia xanthochymus Hook. for weed management. Applied Sciences, 11, 2264. doi: 10.3390/app11052264
Rusu, M.E., Gheldiu, A.M., Mocan, A., Moldovan, C., Popa, D.S., Tomuta, I., & Vlase, L. (2018). Process optimization for improved phenolic compounds recovery from walnut (Juglans regia L.) septum: phytochemical profile and biological activities. Molecules, 23, 2814. doi: 10.3390/molecules23112814
Santos, A., Barros, L., Calhelha, R.C., Duenas, M., Carvalho, A.M., Santos-Buelga, C., & Ferreira, I.C.F.R. (2013). Leaves and decoction of Juglans regia L.: Different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Industrial Crops and Products, 51, 430/436. doi: 10.1016/j.indcrop.2013.10.003
Shah, U.N., Mir, J.I., Ahmed, N., Jan, S., & Fazili, K.M. (2018). Bioefficacy potential of different genotypes of walnut Juglans regia L. Journal of Food Science and Technology, 55, 605-618. doi: 10.1007/s13197-017-2970-4
Simoes, K., Du, J., Kretzschmar, F.S., Broeckling, C.D., Stermitz, F.S., Vivanco, J.M., & Braga, M.R. (2008). Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata. Journal of Chemical Ecology, 34, 681-687. doi: 10.1007/s10886-008-9443-1
Strugstad, M., & Despotovski, S. (2012). A summary of extraction, synthesis, properties, and potential uses of juglone: A literature review. Journal of Ecosystems and Management, 13, 1-16.
Wegiera, M., Grabarczyk, P., Baraniak, B., & Danuta Smolarz, H. (2011). Antiradical properties of extracts from roots, leaves and fruits of six Rumex L. species. Acta Biologica Cracoviensia Series Botanica, 53(1), 125-131. doi: 10.2478/v10182-011-0018-z
Wegiera, M., Smolarz, H. D., Wianowska, D. & Dawidowicz, A. L. (2007). Anthracene derivatives in some species of Rumex L. genus. Acta Societatis Botanicorum Poloniae, 76, 103-108. doi: 10.5586/asbp.2007.013
Zaller, J.G. (2006). Allelopathic effects of Rumex obtusifolius leaf extracts against native grassland species. Journal of Plant Diseases and Proctection, 20, 463-470.
Zhou, Y., Yang, B., Jiang, Y., Liu, Z., Liu, Y., Wang, X., & Kuang, H. (2015). Studies on cytotoxic activity against HepG-2 cells of naphthoquinones from green walnut husks of Juglans mandshurica Maxim. Molecules, 20(9), 15572–15588. doi: 10.3390/molecules200915572
Zubay, P., Kunzelmann, J., Ittzés, A., Németh Zámboriné, É., & Szabó, K. (2021). Allelopathic effects of leachates of Juglans regia L., Populus tremula L. and juglone on germination of temperate zone cultivated medicinal and aromatic plants. Agroforestry Systems, 95, 431-442. doi: 10.1007/s10457-020-00572-9
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