Micronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides
Abstract
Micronucleus (MN) is a biomarker widely used in biomonitoring studies for determining
the genetic risk associated with exposure to pesticides. The purpose of this study was to
assess damage to the genetic material of workers occupationally exposed to pesticides as detected in micronucleus tests. The research included 119 subjects divided into three groups: a control group of 39 subjects, a group of 40 subjects exposed to pesticides as producers and a group of 40 pesticide applicators in the field. A Mann-Whitney U-test displayed statistically significant differences between the parameter means of all variables, and the control group. Significant differences were observed between males involved in pesticide production and application for the parameter MN4, then between non-smoking producers and applicators regarding parameters MN2, MN3, MN4 and NB, as well as between the control and applicator groups for parameter MN2, and between producers and applicators for parameter MN3. Spearman’s correlation test showed a positive correlation between the frequency of micronuclei and age of respondents, as well as their smoking habits. A statistically significant difference in relation to cytogenetic parameters was detected between the respondents working in pesticide production and those working in the field. The results suggest that applicators in the field do not use adequate personal protective equipment. Regular biological monitoring of workers exposed to pesticides is required.
References
Albertini, R.J., Nicklas, J.A., & O’Neill, J.P. (1996). Future research direction for evaluating human genetic and cancer risk from environmental exposures. Environmental Health Perspectives, 104 (supplement 3), 503-510.
Baker, E.L., Zack, M., Miles, J.W., Alderman, L., Warren, M.W., Dobbin, R.D., & Miller, S. (1978). Epidemic malathion poisoning in Pakistan malaria workers. The Lancet, 311(1), 31-34.
Bhalli, J. A., Khan, Q. M., Haq, M. A., Khalid, A. M., & Nasim, A. (2006). Cytogenetic analysis of Pakistani individuals occupationally exposed to pesticides in a pesticide production industry. Mutagenesis, 21(2), 143-148. doi:10.1093/mutage/gel009
Bolognesi, C. (2003): Genotoxicity of pesticides: A review of human biomonitoring studies. Mutation Research, 543(3), 251-272. doi:10.1016/S1383-5742(03)00015-2
Bolognesi, C., Parrini, M., Reggiardo, G., Merlo, F., & Bonassi, S. (1993). Biomonitoring of workers exposed to pesticides. International Archives of Occupational and Environmental Health, 65(1), 185-187. doi: 10.1007/BF00381337
Bonassi, S., Bolognesi, C., Abbondandolo, A., Barale, R.,Bigatti, P., Camurri, L., …. Puntoni, R. (1995). Influence of sex on cytogenetic end points – Evidence from a large human sample and review of the literature. Cancer Epidemiology, Biomarkers and Prevention, 4, 671-679.
Bonassi, S., Fenech, M., Lando, C., Lin, Y-P., Ceppi, M., Chang, W.P., … Zijno, A. (2001). Human MicroNucleus Project: International database comparison for results
with the cytokinesis-block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei.
Environmental and Molecular Mutagenesis, 37(1), 31-45. doi: 10.1002/1098-2280(2001)37:1<31:AIDEM1004>3.0.CO;2-P
Bonassi, S., Neri, M., Lando, C., Ceppi, M., Lin, Y-P., Chang, W.P., … the HUMN collaboratoive group (2003). Effect of smoking habit on the frequency of micronuclei in human lymphocytes: Results from human MicroNucleus Project. Mutation Research, 543(2), 155-166. doi:10.1016/S1383-5742(03)00013-9
Bonner, M.R., Williams, B.A., Rusiecki J.A., Blair, A., Beane Freeman, L.E., Hoppin, J.A., … Alavanja, M.C. (2010). Occupational exposure to terbufos and the incidence of cancer in the agricultural health study. Cancer Causes and Control, 21(6), 871-877. doi: 10.1007/S10552-010-9514-9
Bull, S., Fletcher, K., Boobis, A.R., & Battershill, J.M. (2006). Evidence for genotoxicity of pesticides in pesticide applicators: A review. Mutagenesis, 21(2), 93-103. doi:10.1093/mutage/gel011
Carbonell, E., Puig, M., Xamena, N., Creus, A., & Marcos, R. (1990). Sister chromatid exchanges in lymphocytes of agricultural workers exposed to pesticides. Mutagenesis,
(4), 403-405. doi:10.1093/mutage/5.4.403
Costa, C., Teixeira, J.P., & Silva, S. (2006). Cytogenetic and molecular biomonitoring of a Portuguese population exposed to pesticides. Mutagenesis, 21(5), 343-350. doi:10.1093/mutage/gel039
Da Silva Augusto, L. G., Lieber, S. R., Ruiz, M. A., & de Souza, C. A (1997). Micronucleus monitoring to a ssess human occupationa l exposure to organochlorides. Environmental and Molecular Mutagenesis, 29(1), 4 6 -52 . doi:10.10 02/(SICI)1098-2280(1997)29:1<46:AID-EM6>3.0.CO;2-B
Fenech, M. (1993). The cytokinesis-block micronucleus technique: A detailed description of the method and its application to genotoxicity studies in human populations. Mutation Research, 285(1), 35-44. 0027-5107/93/$06.00
Fenech, M. (2002). Chromosomal biomarkers of genomic instability relevant to cancer. Drug Discovery Today, 7(22), 1128-1137. doi:S1359-6446(02)02502-3
Fenech, M., Kirsh-Volders, M., Natarajan, A.T., Surralles, J., Crott, J.W., Parrz, J., … Thomas, P. (2011). Molecular mechanisms of micronucleus, nucleoplasmic bridge and
nuclear bud formation in mammalian and human cells. Mutagenensis, 26(2), 125-132. doi:10.1093/mutage/geq052
Garaj-Vrhovac, V., & Zeljezdić, D. (2002). Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and comet assay. Journal of Applied Toxicology, 22(4), 249-255. doi:10.1002/jat.855
Grover, P., Danadevi, K., Mahboob, M., Rozati, R.B., Banu, B.S., & Rahman, M.F. (2003). Evaluation of genetic damage in workers employed in pesticide production utilizing the comet assay. Mutagenesis, 18(2), 201-205. doi:10.1093/mutage/18.2.201
Hagmar, L., Brogger, A., Hansteen, I.-L., Heim, S., Högstedt B., Knudsen L., … Sorsa M. (1994). Cancer risk in humans predicted by increased levels of chromosomal aberrations in lymphocytes: Nordic study group on the health risk of chromosome damage. Cancer Research, 54(11), 2919-2922.
Hagmar, L., Stromberg, U., Bonnasi, S., Hasteen, I.L., Knudsen, L., Lindholm, C., & Norppa, H. (2004). Impact of types of lymphocyte chromosomal aberrations on human cancer risk: Results from Nordic and Italian cohorts. Cancer Research, 64(6), 2258-2263. doi:10.1158/0008-5472.CAN-3-3360
International Atomic Energy Agency (IAEA). (2001). Cytogenetic analysis for radiation dose assessment: A manual. In Technical Report Series No 405. Vienna, Austria: IAEA
Javed, A., Bhalli A., Khan, Q.M., Haq, A., Khalid, M., & Nasim, A. (2006). Cytogenetic analysis of Pakistani individuals occupationally exposed to pesticides in a pesticide production industry. Mutagenesis, 21(2), 143-148. doi:10.1093/mutage/gel009
Jovicic, D., Milacic, S., Vukot T., Rakic, B., Stevanovic, M., Drakulic, D., … Bukvic, N. (2010). Detection of premature segregation of centromeres in persons exposed to ionizing radiation. Health Physics, 98 (5), 717-727.
Jovičić, D., Pajić J., Rakić, B., Radivojević, Lj., Pajić M., Janjić, V., & Milovanović, A. (2013). Cytogenetic biomonitoring in a Serbian population occupationally exposed to a complex mixture of pesticides. Genetika, 45(1), 121-133. doi:10.2298/GENSR1301121J
Lucero, L., Pastor, S., Suarez, S., Durban, R., Gomez, C., Parron, T., … Marcos, R. (2000). Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides:
Micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells. Mutation Research, 464(2), 255-262. doi: 10.1016/S1383-5718(99)00200-4
Mateuca, R., Lombaert, N., Aka, P.V., Decodier, I., & Kirsch-Volders M. (2006). Chromosomal changes: Induction, detection methods and applicability in human biomonitoring. Biochemie, 88(11), 1515-1531.
Matsuoka, S., Huang, M. & Elledge, S. J. (1998). Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science, 282 (5395), 1893-1897.
Migliore, L., Parrini, M., Sbrana, J., Biagini, C., Battaglia, A., & Loprieno, N. (1991). Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: The age effects. Mutation Research, 256(1), 13-20.
Pasastor, S., Creus, A., Xamena, N., Siffel, C., & Marcos, R. (2002). Occupational exposure to pesticides and cytogenetic damage: Results of a Hungarian population
study using the micronucleus assay in lymphocytes and buccal cells. Environmental and Molecular Mutagenesis, 40(2), 101-109. doi: 10.1002/em.10100
Radivojević, Lj., Gašić S., Šantrić, Lj., Gajić Umiljendić, J., & Marisavljević, D. (2012). Short-time effects of the herbicide nicosulfuron on the biochemical activity of chernozem soil. Journal of the Serbian Chemical Society, 77(6), 845-854. doi: 10.2298/JSC110825004R
Radivojević, Lj., Gašić, S., Šantrić, Lj., & Stanković-Kalezić, R. (2008). The impact of atrazine on several biochemical properties of chernozem soil. Journal of the Serbian Chemical Society, 73(10), 951-959. doi:10.2298/JSC0810951R
Ros, M., Goberna, M., Moreno, J.L., Hernandez, T., Garcia, C., Insam, H., & Pascual, J.A. (2006). Molecular and physiological bacterial diversity of a semi-arid soil contaminated with different levels of formulated atrazine. Applied Soil Ecology, 34(2-3), 93-102. doi:10.1016/j.apsoil.2006.03.010
Rupa, D.S., Rita, P., Reddy, P.P., & Reddy, O.S. (1988). Screening of chromosomal aberrations and sister chromatid exchanges in peripheral lymphocytes of vegetable garden workers. Human Toxicology, 7(4), 333-336.
Shaham, J., Kaufman, Z., Gurvich, R., & Levi, Z. (2001). Frequency of sister-chromatid exchange among greenhouse farmers exposed to pesticides. Mutatation Research, 491(1-2), 71-80.
Sorsa, M., Wilbourn, J., & Vainio, H. (1992). Human cytogenetic damage as a predictor of cancer risk. In H. Vainio, P. Magee, D. McGregory & A. McMichael (Eds), Mechanisms of Carcinogenesis in Risk Identification, IARC Scientific Publications No. 116 (pp 543-554). Lyon, France: IARC.
Tomlin, C.D.S. (Ed.). (2009). The pesticide manual: A world compendium. (15th ed.). Alton, UK: British Crop Protection Council.
Tucker, P., Haig-Ferguson, H., Eaton, N., & Crawley, E. (2011). What to do about attention and memory problems in children with CFS/ME: A neuropsychological approach. Clinical Child Psychology and Psychiatry, 16(2), 215-223. doi: 10.1177/1359104511403585
World Health Organisation (WHO), (2004). The WHO recommended classification of pesticides and guidelines to classification. Geneva, Switzerland: WHO.
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