The presence of Turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia

  • Dragana N Milošević Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
  • Maja Ignjatov Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
  • Zorica Nikolic Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
  • Ivana Stankovic University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
  • Aleksandra Bulajic University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
  • Ana Marjanovic-Jeromela Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
  • Branka Krstic University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Keywords: Oilseed rape, Turnip yellows virus, Molecular detection, Serbia,

Abstract


A total of 86 oilseed rape samples from six crops in different localities were collected
during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.

Author Biographies

Dragana N Milošević, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
Laboratory for seed testing
Maja Ignjatov, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
Laboratory for seed testing
Zorica Nikolic, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad
Laboratory for seed testing

References

Association for the Promotion of Production and Exports of Grains and Oilseeds (n.d.). Retrieved from “Cereals Serbia” at www.zitasrbije.rs

Duffus, J.E. & Russell, G.E. (1972). Serological relationship between Beet western yellows and Turnip Yellows Virus. Phytopathology, 62, 1274-1277.

Ebrahim-Ghomi, M. (2014). Study on distribution and detection of cauliflower mosaic virus (CaMV) in Dezful region of Iran. International Journal of Biosciences, 4(11), 271-275. doi:10.12692/ijb/4.11.271-275

Graichen, K. & Peterka, H. (1999). Generation of oilseed rape with resistance to turnip yellows luteovirus (p 148). In 10th International Rapeseed Congress - New Horizons from an Old Crop. Canberra, Australia: GCIRC.

Graichen, K. & Rabenstein, F. (1996). European isolates of Beet western yellows virus (BWYV) from oilseed rape (Brassica napus L. ssp. napus) are non-pathogenic on sugar beet (Beta vulgaris L. var. altissima) but represent isolates of Turnip yellows virus (TuYV). Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz – Journal of Plant Diseases and Protection, 103, 233-245.

Graichen, K. & Schliephake, E. (1999). Infestation of winter oilseed rape by Turnip yellows luteovirus and its effect on yield in Germany (p131). In 10th International Rapeseed Congres - New Horizons from an Old Crop. Canberra, Australia: GCIRC.

Hardwick, N.V., Davies J.M.L. & Wright D.M. (1994). The incidence of three virus diseases of winter oilseed rape in England and Wales in the 1991/92 and 1992/93 growing seasons. Plant Pathotogy, 43, 1045-1049. doi:10.1111/j.1365-3059.1994.tb01656.x

Hauser, S., Stevens, M., Beuve, M. & Lemaire, O. (2002). Biological properties and molecular characterization of Beet chlorosis virus (BChV). Archives of Virology, 147, 745-762. doi:10.1007/s007050200023

Hauser, S., Stevens, M., Mougel, C., Smith, H. G., Fritsch, C., Herrbach, E. & Lemaire, O. (2000). Biological, serological and molecular variability suggest three distinct polerovirus species infecting beet or rape. Phytopathology, 90(5), 460-466. doi:10.1094/PHYTO.2000.90.5.460

Hill, S.A., Lane, A. & Hardwick, N.V. (1989). The incidence and importance of Beet western yellows virus in oilseed rape. Aspects of Applied Biology, 23, 311-318.

Jay, C. N., Rossall, S. & Smith, H. G. (1999). Effects of Beet western yellows virus on growth and yield of oilseed rape (Brassica napus). Journal of Agricultural Science, 133,131-139.

Johnstone, G.R., Ashby, J.W., Gibbs, A.J., Duffus, J.E., Thottapilly, G. & Fletcher J. D. (1984). The host ranges, classification and identification of eight persistent aphid-transmitted viruses causing diseases in legumes. Netherlands Journal of Plant Pathology, 90(6), 225-245. doi:10.1007/BF01976381

Johnstone, G.R., & Duffus, J.E. (1984). Some luteovirus diseases in Tasmania caused by Beet western yellows and subterranean clover red leaf viruses. Australian Journal of Agricultural Research, 35, 821-830.

Jones, R.A.C., Coutts, B.A. & Hawkes, J. (2007). Yieldlimiting potential of Beet western yellows virus in Brassica napus. Australian Journal of Agricultural Research, 58, 788-801. doi:10.1071/AR06391

King, A.M.Q., Adams, M.J., Carstens, E.B. & Lefkowitz, E.J. (Eds.) (2011). Virus Taxonomy: Ninth report of the International Committee on Taxonomy of Viruses. San Diego, CA: Elsevier Academic Press.

Latham, L.J., Smith, L.J. & Jones, R.A.C. (2003). Incidence of three viruses in vegetable brassica plantings and associated wild radish weeds in south-west Australia. Australasian Plant Pathology, 32, 387-391. doi:10.1071/AP03031

Mayo, M.A. (2002). ICTV at the Paris ICV: results of the plenary session and the binomial ballot. Archives of Virology, 147, 2254-2260. doi:10.1007/s007050200052

Milošević, D., Marjanović-Jeromela, A., Ignjatov, M., Jovičić, D., Stanković, I., Bulajić A. & Krstić B. (2015). First report of Turnip yellows virus on oilseed rape in Serbia. Plant disease, 99(12), 1869. doi:10.1094/PDIS-05-15-0503-PDN

Raymer, P.L. (2002). Canola: An emerging oilseed crop (pp 122-126). In J. Janick and A. Whipkey (eds.), Trends in new crops and new uses. Alexandria, VA: ASHS Press.

Schliephake, E., Graichen, K. & Rabenstein, F. (2000). Investigations on the vector transmission of the Beet mild yellowing virus (BMYV) and the Turnip yellows virus (TuYV). Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz – Journal of Plant Diseases and Protection, 107, 81-87.

Schubert, J., Rabenstein, F., Graichen, K. & Richter, K. (1998). Comparison of the 5ｴ-end nucleotide sequences of luteoviruses from oilseed rape and sugarbeet. Archives of Phytopathology and Plant Protection 31, 519-530. doi:10.1080/03235409809383265

Shahraeen, N., (2012). An overview of oilseed rape (canola) virus diseases in Iran. International Research Journal of Microbiology, 3(1), 024-028.

Statistical Office of the Republic of Serbia (2014). Retrieved from www.stat.gov.rs

Stevens, M., Smith, H.G. & Hallsworth, P.B. (1994). The host-range of beet yellowing viruses among common arable weed species. Plant Pathology, 43, 579-588. doi:10.1111/j.1365-3059.1994.tb01593.x

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731-9. doi:10.1093/molbev/msr121

Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673-4680. doi:10.1093/nar/22.22.4673

USDA (2011). Oilseeds: World markets and trade. Retrieved from USDA Foreign Agricultural Service at http://www.fas.usda.gov/data/oilseeds-world-markets-and-trade.

Walsh, J.A., Perrin, R.M., Miller, A. & Laycock, D.S. (1989). Studies of beet western yellows virus in winter oilseed rape (Brassica-napus ssp oleifera) and the effect of insecticidal treatment on its spread. Crop Protection, 8, 137-143. doi:10.1016/0261-2194(89)90090-2

Walsh, J.A. & Tomlinson, J.A. (1985). Viruses infecting winter oilseed rape (Brassica napus ssp. oleifera). Annals of Applied Biology, 107, 485-495. doi:10.1111/j.1744-7348.1985.tb03165.x

Published
2016/06/24
How to Cite
Milošević, D. N., Ignjatov, M., Nikolic, Z., Stankovic, I., Bulajic, A., Marjanovic-Jeromela, A., & Krstic, B. (2016). The presence of Turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 31(1-2). Retrieved from https://aseestant.ceon.rs/index.php/pif/article/view/9908
Issue
Vol. 31 No. 1-2 (2016): Pesticidi i fitomedicina
Section
Original Scientific Paper

Authors retain copyright of the published papers and grant to the publisher the non-exclusive right to publish the article, to be cited as its original publisher in case of reuse, and to distribute it in all forms and media.

The published articles will be distributed under the Creative Commons Attribution ShareAlike 4.0 International license (CC BY-SA). It is allowed to copy and redistribute the material in any medium or format, and remix, transform, and build upon it for any purpose, even commercially, as long as appropriate credit is given to the original author(s), a link to the license is provided, it is indicated if changes were made and the new work is distributed under the same license as the original.

Users are required to provide full bibliographic description of the original publication (authors, article title, journal title, volume, issue, pages), as well as its DOI code. In electronic publishing, users are also required to link the content with both the original article published in Pesticidi i fitomedicina (Pesticides and Phytomedicine) and the licence used.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.