In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

  • Natalia Georgieva Institute of Forage Crops, 89 General Vladimir Vazov Str., Pleven
  • Ivelina Nikolova Institute of Forage Crops, 89 General Vladimir Vazov Str., Pleven
  • Valentin Kosev Institute of Forage Crops, 89 General Vladimir Vazov Str., Pleven
  • Yordanka Naydenova Institute of Forage Crops, 89 General Vladimir Vazov Str., Pleven
DOI: https://doi.org/10.2298/pif.v32i1.12862
Keywords: Organic fertilizers, Pollen, Germination, Peas,

Abstract


The objective of this study was to evaluate the influence of two organic nanofertilizers,
Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

Author Biography

Natalia Georgieva, Institute of Forage Crops, 89 General Vladimir Vazov Str., Pleven
Associated professor

References

Bhangoo, M.S., Day, K. S., Sudanagunta, V. R., & Petrucet ,V.E. (1988). Application of poultry manure influence on Thompson seedless grape production and soil properties. HortScience, 23(6), 1010-1012.

Bolat, I., & Pirlak, L. (1999). An investigation on pollen viability, germination and tube growth in some stone fruits. Turkish Journal of Agriculture and Forestry, 23(4), 383-389.

Buyukkartal, H.N. (2003). In vitro pollen germination and pollen tube characteristics in tetraploid red clover (Trifolium pratense L.). Turkish Journal of Botany, 27, 57-61.

Coser, S.M., Fontes, M.M.P., & Ferreira, M.F.S. (2012). Assessment of pollen viability in guava genotypes. Acta Horticulturae, 959, 141-144. Retrieved from http://www.actahort.org/books/959/959_17.htm

Cresti, M., & Tiezzi, A. (1992). Sexual plant reproduction. In E. S. Pierson & Y. Q. Li (Eds.), The cytoskeleton of pollen grains and pollen tube (pp. 99-113). Berlin, Germanz: Springer-Verlag. Retrieved from ttps://link.springer.com/chapter/10.1007%2F978-3-642-77677-9_10

Dane, F., Olgun, G., & Dalgic, O. (2004). In vitro pollen germination of some plant species in basic culture medium. Journal of Cell and Molecular Biology, 3, 71-76.

Franzon, R.C., Correa, E.R., & Raseira, M.C.B. (2005). In vitro pollen germination of feijoa (Acca sellowiana (Berg) Burret). Crop Breeding and Applied Biotechnology, 5(2), 229-233. doi 10.12702/1984-7033.v05n02a14.

Gwata, E.T., Wofford, D.S., Pfahler, P.L., & Boote, K.J. (2003). Pollen morphology and in vitro germination characteristics of nodulating and non-nodulating soybean (Glicine max L.) genotypes. Theoretical and Applied Genetics, 106(5), 837-839. doi:10.1007/s00122-002-1097-8

Hassan, A.M., Abd-Alhamid, N., Rawheya Aly, B.M.A., Hassan, H.S.A., Abdelhafez, A.A., & Laila, F.H. (2015). Effect of organic and bio-fertilization on yield and quality of „Manzanillo“ olives. Middle East Journal of Agriculture Research, 4(3), 485-493.

Khosh-Khui, M., Bassiri A., & Niknejad, M. (1976). Effects of temperature and humidity on pollen viability of six rose species. Canadian Journal of Plant Science, 56(3), 517-523.

Manjunatha, S.B., Biradar, D.P., & Aladakatti, Y.R. (2016). Nanotechnology and its applications in agriculture: A review. Journal of Pharmaceutical Sciences, 29(1), 1-13. Retrieved from http://14.139.155.167/test5/index.php/kjas/article/viewFile/7890/8148

Martin, F.W. (1972). In vitro measurement of pollen tube growth inhibition. Plant Physiology, 49(6), 924-925.

Neves, T.S., Machado, G.M.E., & Oliveira, R.P. (1997). Efeito de diferentes concentracoes de carboidratos e acido borico na germinacao de graos de polen de cubiuzeiro e cupuacuzeiro. Revista Brasileira de Fruticultura, 19, 207-211.

Nikolova, V., Angelova, S., Kalapchieva, S., Petkova, V., & Stoeva, V. (2012). High temperature influence on the pollen viability of Pisum sativum L. accessions. Agrarni nauki / Agricultural Sciences, 4(8), 74-80.

Pavlov, D., & Kostov, K. (2001). Forage production (3rd edition). Plovdiv, Bulgaria: Agrarian Univeristy Press. (In Bulgarian)

Salles, L.A., Ramos, J.D., Pasqual, M., Junqueira, K.P., & Silva, A.B. (2006). Sucrose e pH na germinacao in vitro de graos de polen de citros. Revista Ciência e Agrotecnológia, 30: 170-174. Retrieved from https://dx.doi.org/10.1590/S1413-70542006000100025

Sekhon, B.S. (2014). Nanotechnology in agri-food production: an overview. Nanotechnology, Science and Applications, 7, 31-53. doi 10.2147/NSA.S39406.

Sharafi, Y., Motallebbi-Azar, A., & Bahmani, A. (2011). In vitro pollen germination, pollen tube growth and longevity in some genotypes of loquat (Eriobotria japonica Lindl.). African Journal of Biotechnology, 10(41): 8064-8069. doi 10.5897/AJB11.701.

Sheykhbaglou, R., Sedghi, M., Shishevan, M.T., & Sharifi, R.S. (2010). Effects of nano-iron oxide particles on agronomic traits of soybean. Notulae Scientia Biologicae, 2(2), 112–113. doi 10.15835/nsb.2.2.4667

Soares, T. L., de Oliveira e Silva, S., de Carvalho Costa, M.A.P., Santos-Serejo, J.A., da Silva Souza, A., Lino, L. S. M. ... de Jesus, O.N. (2008). In vitro germination and viability of pollen grains of banana diploids. Crop Breeding and Applied Biotechnology, 8, 111-118.

Stanley, R.G., & Linskens, H.F. (1974). Pollen: Biology, biochemistry, management. Berlin, Germany: Springer-Verlag.

Wang, Z.Y., Yaxin, G., Scott, M., & Spangenberg, G. (2004). Viability and longevity of pollen from transgenic and nontransgenic tall fescue (Festuca arundinacea) (Poaceae) plants. American Journal of Botany, 91, 523-530. doi 10.3732/ajb.91.4.523

Warnock, S. J., & Hagedorn, D. J. (1956). Germination and storage of pea (Pisum sativum L.) pollen. Agronomy Journal, 48(8), 347-352. doi 10.2134/agronj1956.00021962004800080004x

Published
2017/06/05
Issue
Vol. 32 No. 1 (2017): Pesticidi i fitomedicina
Section
Original Scientific Paper

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