Molecular Typing of RhD-Negative Blood Donors with C and/or E Antigen

  • Gordana Guzijan Zavod za transfuzijsku medicinu Republike Srpske
DOI: https://doi.org/10.5937/scriptamed50-22635

Abstract


Background: Most people are either RhD positive or RhD negative, but there is also a number of persons with D antigen variants. Aim: To prove, by using molecular diagnostic tests, whether the RHD gene and D antigen on the red cell membrane of the blood donors serologically typed as RhD-negative with RhD phenotype Ccddee and ccddEe, are so weak that they cannot be proven by serology techniques or the available anti-D test serums. Methods: Samples used are those of regular voluntary donors who were serotyped as RhD-negative, C and/or E positive. Samples were collected from voluntary donors at the Institute for Transfusion Medicine of the Republic of Srpska during the period from April 2016 to December 2018. Results: Among the serologically proven RhD-negative donors, 346 had C and/or E in their phenotype and those were subjected to molecular screening test. Conclusion: The first results of molecular typing match those published in literature, i.e. the RHD gene is present in some serologically RhD-negative forms, which was proven by molecular testing.

Keywords: Rh system, D antigen, weak D, partial D

Author Biography

Gordana Guzijan, Zavod za transfuzijsku medicinu Republike Srpske
Dr med sci
Published
2019/08/08
Issue
Vol. 50 No. 2 (2019)
Section
Original article
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