Time-dependent and force-dependent vasoreactivity of isolated human umbilical arteries
Abstract
Background/Aim: There have been different experimental conditions for in vitro studies on human umbilical arteries (HUA) in tissue bath system. This diversity was mainly reflected in variables such as stretching tension, incubation period and initial constriction challenging with potassium (KCl). The aim of the study was to establish optimal experimental conditions which will provide better responsiveness of HUA preparations, as well as to examine the impact of 24 h cold storage on viability and responsiveness of HUA to KCl and serotonin.
Methods: The KCl-induced constrictions at different stretching tensions (0.5 g, 1.0 g, 2.0 g, 4.0 g), incubation times (30 min, 60 min, 120 min), and after multiple initial constriction challenging were compared. Dose response curves for serotonin were obtained under different conditions (1.0 g and 60 min vs. 2.0 g and 120 min). The influence of 24 h cold storage on KCl- and serotonin- induced vasoconstriction of HUA preparations was examined as well.
Results: The strongest constrictions induced by serotonin or KCl were obtained when preparations were adjusted at 2.0 g and incubated for 120 min. The KCl-induced constrictions observed after 120 min were statistically higher (p < 0.05) when preparations were challenged three times (30 min, 60 min, 120 min), compared to those challenged only once. The preparations that were stored at 4 ⁰C for 24 h showed significantly stronger serotonin-induced constrictions (p < 0.01). The cold storage had no influence on KCl-induced constriction.
Conclusion: For performing in vitro studies on HUA preparations in tissue bath, we propose stretching tension of 2.0 g, incubation period of 120 min and multiple initial constriction challenging with KCl as optimal experimental condition. We also showed that HUA preparations retained functional viability even after 24 h of cold storage.
References
2. Hussain A, Bennett RT, Chaudhry MA, et al. Characterization of optimal resting tension in human pulmonary arteries. World J Cardiol 2016;8(9):553-8.
3. Rodegra H, Roder G, Schmermund HJ, Soering K. Wirkung von 5-Hydroxytryptamin auf die isolierte Nabelschnurarterie [Effect of 5-hydroxytryptamine on the isolated umbilical cord artery]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol 1957;232(1):285-6.
4. Tong W, Giussani DA. Preeclampsia link to gestational hypoxia. J Dev Orig Health Dis 2019;10(3):322-33.
5. Reilly FD, Russell PT. Neurohistochemical evidence supporting an absence of adrenergic and cholinergic innervation in the human placenta and umbilical cord. Anat Rec 1977;188(3):277-286.
6. Bertrand C, St-Louis J. Reactivities to serotonin and histamine in umbilical and placental vessels during the third trimester after normotensive pregnancies and pregnancies complicated by preeclampsia. Am J Obstet Gynecol 1999;180(3 Pt 1):650-9.
7. Bertrand C, Duperron L, St-Louis J. Umbilical and placental vessels: modifications of their mechanical properties in preeclampsia. Am J Obstet Gynecol 1993;168(5):1537-46.
8. Altura BM, Malaviya D, Reich CF, Orkin LR. Effects of vasoactive agents on isolated human umbilical arteries and veins. Am J Physiol 1972;222(2):345-55.
9. Monuszko E, Halevy S, Freese K, Liu-Barnett M, Altura B. Vasoactive actions of local anaesthetics on human isolated umbilical veins and arteries. Br J Pharmacol 1989;97(2):319-28.
10. Tufan H, Ayan-Polat B, Tecder-Unal M, Polat G, Kayhan Z, Oğüş E. Contractile responses of the human umbilical artery to KCl and serotonin in Ca-free medium and the effects of levcromakalim. Life Sci 2003;72(12):1321-9.
11. Dayioglu E, Buharalioglu CK, Saracoglu F, Akar F. The effects of bumetanide on human umbilical artery contractions. Reprod Sci 2007;14(3):246-52.
12. Templeton AG, McGrath JC, Whittle MJ. The role of endogenous thromboxane in contractions to U46619, oxygen, 5-HT and 5-CT in the human isolated umbilical artery. Br J Pharmacol 1991;103(1):1079-84.
13. Kleszczewski T, Buzun L, Lisowska A, Modzelewska B. Potassium induced contraction of the internal thoracic artery in vitro is time related: the potential consequences in the analysis of the mechanism of the spasm after coronary artery bypass grafting and in the analysis of the results of in vitro studies. Heart Vessels 2016;31(4):616-21.
14. Wright G. Use-dependent decline in rat aorta sensitivity to contraction by potassium. Can J Physiol Pharmacol 1991;69(7):921-8.
15. Jones JB, Rowsell A. Fetal 5-hydroxytryptamine levels in late pregnancy. J Obstet Gynaecol Br Commonw. 1973;80(8):687-9.
16. Okatani Y, Wakatsuki A, Reiter RJ. Melatonin suppresses homocysteine enhancement of serotonin-induced vasoconstriction in the human umbilical artery. J Pineal Res 2001;31(3):242-7.
17. Sinanović O, Chiba S. Responsiveness of skeletal muscle branches of the dog femoral artery to alpha-adrenoceptor agonists before and after cold storage. Arch Int Pharmacodyn Ther 1987;287(1):146-57.
18. (Sinanović O, Chiba S. Responsiveness of monkey skeletal muscle arteries to vasoconstrictor substances before and after cold storage. Jpn J Pharmacol 1988;46(3):237-46.
19. Güney HZ, Ercan ZS. Prolonged cold storage diminishes the 5-hydroxytryptamine- and potassium chloride-mediated contractions of rat thoracic aorta. Tohoku J Exp Med 2009;217(2):155-61.
20. Kevelaitis E, Nyborg NC, Menasché P. Coronary endothelial dysfunction of isolated hearts subjected to prolonged cold storage: patterns and contributing factors. J Heart Lung Transplant 1999;18(3):239-47.
21. Abad A, Estañ L, Morales-Olivas FJ, Serra V. Reactivity of isolated human chorionic vessels: analysis of some influencing variables. Can J Physiol Pharmacol 2003;81(12):1147-51.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).