Effect of Hydrogen Sulphide Containing Mineral Water on Experimental Osteoporosis in Rats

  • Tamara Popović doc dr , Zavod za fizikalnu medicinu i rehabilitaciju "dr Miroslav Zotović", Slatinska 1, Banjaluka 78000
  • Ljiljana Amidžić
  • Mile Čeko
  • Siniša Marković
  • Ranko Škrbić
DOI: https://doi.org/10.5937/scriptamed53-41462
Keywords: Osteoporosis, Hydrogen-sulphide, Sulphur water, Bone metabolism

Abstract


Background/Aim. Sulphur mineral water is widely used in the treatment of musculoskeletal diseases. Hydrogen sulphide is an important regulator of bone metabolism and its application in the treatment of osteoporosis is intensively researched. The aim of this study was to analyse biochemical and histological effects of H2S containing mineral water of “Mlječanica” spring on ovariectomy-induced experimental osteoporosis in rats.

Methods. In this experiment a 14-week-old Wistar female rats were used. The animals undergone bilateral ovariectomy (OVX groups) as an experimental model for oestrogen-deficient osteoporosis. After six weeks, animals were divided into control and the experimental group. Rats from the experimental group treated with H2S (SW group) containing mineral water ad libitum during five weeks. Biochemical parameters for monitoring sulphur water effects were concentration in serum of osteocalcin, alkaline phosphatase, calcium and phosphorus. Histological analyses of the left tibia coloured with haematoxylin-eosin were carried out.

Results. Regarding the biochemical parameters, a statistically significant increase was observed in the OVX group for osteocalcin, alkaline phosphatase calcium and phosphorus compared to the sham-operated (CNT) group (p < 0.01). In SW + OVX, alkaline phosphatase was statistically significantly decreased (p < 0.01) and serum osteocalcin and phosphorus increased (p < 0.01). Calcium values were increased without significance. In the OVX + SW group, histological analyses showed numerous osteoblasts along the trabecular endosteum and the growth of young chondrocytes in the central bone zone and their migration to the peripheral parts.

Conclusion: Drinking the H2S containing “Mlječanica” mineral water has led to decreased alkaline phosphatase, increased osteocalcin and phosphorus concentration in serum and stimulated the bone reparation in osteoporotic rats.

References

1.      Compston EJ, McClug RM, Leslie WD. Osteoporosis. Lancet 2019;393(10169):364-76.

2.      Le Boff MS, Greenspan SL, Insogna KL, Lewiecki EM, Saag KG, et al. The clinician’s guide to prevention and treatment of osteoporosis. Osteop Int 2022;33:2049-102.

3.      Tonk CH, Shoushrah SH, Babczyk P, El Khaldi-Hansen, B. Shultze, M Hertzen, et al. Treatments for osteoporosis–which combination of pills is the best among the bad. Int J Mol Sci 2022;23:1393. doi: 10.3390/ijms23031393.

4.      Li L, Rose P, Moore PK. Hydrogen sulfide and cell signaling. Annu Rev Pharmacol Toxicol 2011;51:169–87.

5.      Gambari L, Grigolo B, Grassi F. Hydrogen sulfide in bone tissue regeneration and repair: state of the art and new perspectives. Int J Mol Sci 2019;20:5231. doi: 10.3390/ijms20205231.

6.      Oerridon BW, Leuvenink HGD, Hillebrands J-L, van Goor H, Bos Eelke M. The role of hydrogen sulfide in aging and age-related pathologies. Aging (Albany NY) 2016;8(10):2264-89.

7.       Xu ZS, Dai F, Chen J,Lv M, Cheng JW, Zhang XM, et al. Experimental research into the potential therapeutic effect of Gyy4137 on ovariectomy-induced osteoporosis. Cell Mol Biol Lett 2018;23:47. doi: 10.1186/s11658-018-0114-0.

8.      Huang TH, Lewis JL, Lin HS, Kuo LT, Mao SW, Tai YS, et al. Methionine-restricted diet and endurance exercise decrease bone mass and extrinsic strength but increase intrinsic strength in growing male rats. J Nutr 2014;144:621–30.

9.      Grassi F, Tyagi AM, Calvert JW, Gambari L, Walker LD, Yu M, et al. Hydrogen sulfide is a novel regulator of bone formation implicated in the bone loss induced by estrogen deficiency. J Bone Miner Res 2016;31:949–63.

10.   Tišma R, Stefanovski G, Stefanovski M. Natural healing sulfur water of the Institute ''Mlječanica''. 1st ed. Laktaši, Bosnia and Herzegovina: Grafomark, 2005.

11.   Gianfaldoni S, Tchernev G, Wollina, Roccia MG, Fioranelli M, Gianfaldoni R, et al. History of the baths and thermal medicine. Open Access Maced J Med Sci 2017;5(4):566–8.

12.   Cacciapuoti S, Luciano MA, Megna M, Annunziata MC, Napolitano M, Patruno C, et al. The role of thermal water in chronic skin diseases management: A review of the literature. J Clin Med 2020 Sep 22;9(9):3047. doi: 10.3390/jcm9093047.

13.   Citi V, Martelli A, Gorica E, Brogi S, Testai L, Calderone V. Role of hydrogen sulfide in endothelial dysfunction: Pathophysiology and therapeutic approaches. J Adv Res 2020 May 19;27:99-113.

14.   Operta M, Hyseni S. Thermal mineral waters in Bosnia and Herzegovina as well as the potential for tourism development. Int J Water Res 2013;1(1):25-09.

15.   Erceg-Rukavina T, Stefanovski M. Balneotherapy in treatment of spastic upper limb afer stroke. Med Arh 2015;69(1):31-3.

16.   Erceg-Rukavina T, Stefanovski M. Effects of sulphate-sulphide mineral water “Mlječanica” in patients with hypertension. Mater Sociomed 2014;26(6):364-65.

17.   Stefanovski M. Balneotherapy of lower extremity joints contractures caused by expolosive injuries. Scr Med 1995;26(1-4):9-12.

18.   Kalu DN. The ovariectomized rat model of post-menopausal bone loss. Bone Miner 1991;15:175-91.

19.   Popović T, Škrbić R, Matavulj M, Obradović Z, Sibinčić S. Expirimental model of osteoporosis on 14-week-old ovariectomised rats: a biochemical, histological and biomechanical study. Biologica Serbica 2016;38(1):18-27.

20.   Waynforth HB. Experimental and surgical technique in the rat. New York: Academic Press 1980; p. 161-163.

21.   Liu Y, Yang R, Liu X, Zhou Y, Qu C, Kikuiri T, et al. Hydrogen sulphide maintains mesenchymal stem cell function and bone homeostasis via regulation of Ca(2+) channel sulfhydration. Cell Stem Cell 2014;15:66–78.

22.   Qin J, Hua Y. Effects of hydrogen sulphide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation. Mol Med Rep 2016;14(4):3871-7.

23.   Vacek TP, Qipshidze N, Tyagi SC. Hydrogen sulfide and sodium nitroprusside compete to activate/deactivate MMPs in bone tissue homogenates. Vasc Health Risk Manag 2013;9:117–23.

24.   Xu ZS, Wang XY, Xiao DM, Hu LF, Lu M, Wu ZY, et al. Hydrogen sulfide protects MC3T3-E1 osteoblastic cells against H2O2-induced oxidative damage implications for the treatment of osteoporosis. Free Radic Biol Med 2011;50:1314–23.

25.   Carbajo JM, Maraver F. Sulphurous mineral waters: new applications for health. Evid Based Complement Alternat Med 2017:8034084. doi: 10.1155/2017/8034084.

26.   Honorio-França AC, De Oliveira FC, França EL, Ferrari CK. Antioxidant and hypoglycemic effects of sulphurous water on alloxan-induced diabetic rats: a preliminary study. Nutr Clín Diet Hosp 2015;35:50–5.

27.   Ahmed HH, Taha FM, Omar HS, Elwi HM, Abdelnasser M. Hydrogen sulfide modulates SIRT1 and suppresses oxidative stress in diabetic nephropathy. Mol Cell Biochem 2019;457:1–9.

28.   Pozsgai G, Benkó R, Barthó L, Horváth K, Pintér E. Thermal spring water drinking attenuates dextran-sulfate-sodium-induced colitis in mice. Inflammopharmacology 2015;23:57–64.

29.   Coruzzi G, Adami M, Pozzoli C, Solenghi E, Grandi D. Functional and histologic assessment of rat gastric mucosa after  chronic treatment with sulphurous thermal water. Pharmacology 2010; 85:146–52.

30.   Costantino M, Filippelli A, Giampaolo C, Tiano L, Carlucci DM, Coiro V, et al. Hypoglycemic and antioxidant activities of sulphureous mineral water. PhOL 2012;1:220–6.

31.   Benedetti S, Benvenuti F, Nappi G, Fortunati NA, Marino L, Aureli T, et al. Antioxidative effects of sulfurous mineral water: protection against lipid and protein oxidation. Eur J Clin Nutr 2009;63:106–12.

32.   Karagülle MZ, Karagülle M. Effects of drinking natural hydrogen sulfide (H2S) waters: a systematic review of in vivo animal studies. Int J Biometeorol 2020;64(6):1011-22.

33.   Erceg-Rukavina T, Dragičević-Cvjetković D. Lipid-reducing effects of sulphate-sulphide mineral water in patients with knee osteoarthritis. Scr Med 2021;52(4):294-8.

34.   Sokrateva TD, Roussev BH, Nashar MA, Kiselova-Kaneva YD, Mihaylova GM, Todorova MN, et al. Effects of sulphur-containing mineral water intake on oxidative status and markers for inflammation in healthy subjects. Arch Physiol Biochem 2021;127(4):327-36.

35.   Carbajo JM, Maraver F. Sulphurous mineral waters: new applications for health. Evid Based Complement Alternat Med 2017:8034084. doi: 10.1155/2017/8034084.

36.   Sunzini F, De Stefano S, Chimenti MS, Melino S. Hydrogen sulfide as potential regulatory gasotransmitter in arthritic diseases. Int J Mol Sci 2020;21:1180. doi: 10.3390/ijms21041180.

Published
2022/12/28
Issue
Vol. 53 No. 4 (2022): Q4/December
Section
Original article
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