The Stromal Derived Factor-1 (SDF-1) Secretion Attracts Stem Cells and Accelerates the Skin Wound Healing Against the Background of Its Closure With Dermal Fibroblasts Associated With Polylactide Film

Alexey  Baranovskiy; Yelena Shapovalova; Boris  Kuzminov; Yuri  Baranovskiy; Igor  Lugin; Lilian  Kutuzova; Nathalia  Pyatigorskaya; Vera  Rostovskaya; Daniil Yuferov; Denis  Kabanov; Grigory Demyashkin

doi:10.5937/scriptamed56-60519

Alexey Baranovskiy Crimean Federal University Named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute Named after S.I. Georgievsky, Simferopol, Russia https://orcid.org/0000-0001-6995-3975
Yelena Shapovalova Crimean Federal University Named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute Named after S.I. Georgievsky, Simferopol, Russia https://orcid.org/0000-0003-2544-7696
Boris Kuzminov Crimean Federal University Named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute Named after S.I. Georgievsky, Simferopol, Russia https://orcid.org/0000-0002-3691-5531
Yuri Baranovskiy Crimean Federal University Named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute Named after S.I. Georgievsky, Simferopol, Russia https://orcid.org/0000-0002-7044-1122
Igor Lugin Crimean Federal University Named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute Named after S.I. Georgievsky, Simferopol, Russia https://orcid.org/0000-0002-9297-9038
Lilian Kutuzova Crimean Federal University Named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute Named after S.I. Georgievsky, Simferopol, Russia https://orcid.org/0000-0002-8448-5476
Nathalia Pyatigorskaya I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia https://orcid.org/0000-0003-4901-4625
Vera Rostovskaya I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia; https://orcid.org/0000-0002-3718-8911
Daniil Yuferov I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia; https://orcid.org/0009-0004-6870-0211
Denis Kabanov I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia https://orcid.org/0009-0006-2496-5706
Grigory Demyashkin I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia https://orcid.org/0000-0001-8447-2600

DOI: https://doi.org/10.5937/scriptamed56-60519

Keywords: Polylactide, film, Skin, Wound healing, Dermal, Fibroblasts, allofibroblasts, Regenerative medicine, SDF-1, Mesenchymal stem cells

Abstract

Background/Aim. Chronic skin wounds are a huge clinical problem, leading to the need for prolonged treatment and significant health care expenses. Regenerative engineering using tissue technologies and cellular constructs offers promising alternatives for treatment. The aim of this research was to evaluate the levels of stromal-derived factor-1 (SDF-1) expression and the presence of CD34+ mesenchymal stem cells (MSC) in regenerating skin wounds treated with polylactide films combined with dermal allofibroblasts.

Methods. Forty-seven white mature male mice line C57/B1 were allocated into two groups – control and experimental. In the experimental group, a standard skin wound was created and treated with polylactide films with allofibroblasts. Biopsies were taken at various time points for histological and immunohistochemical analysis of SDF-1 and CD34 expressions. Cellular indices were determined by counting positive cells per 100 cells under a microscope. Statistical analysis was performed using nonparametric tests.

Results. SDF-1 expression peaked on day 7 and then declined, disappearing by day 15, while the number of MSCs followed a similar trend. Delayed and prolonged SDF-1 expression and the presence of MSCs were observed in control wounds. Enhanced epithelialisation and granulation tissue formation were observed in the experimental group, indicating accelerated wound healing.

Conclusion. Transplantation of polylactide films with dermal allofibroblasts promotes early and active secretion of SDF-1, which promotes MSC recruitment and accelerates skin wound regeneration.

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