Korbazol cytotoxicity against murine cancer cell lines

Suzana Popovic; DEJAN BASKIC; Ivanka Zelen; Predrag Djurdjevic; Milan Zaric; Dusko Avramovic; Nebojsa Arsenijevic

doi:10.5937/sjecr13-2019

Suzana Popovic University of Kragujevac, Medical Faculty
DEJAN BASKIC University of Kragujevac, Faculty of Medical Sciences
Ivanka Zelen University of Kragujevac, Faculty of Medical Sciences
Predrag Djurdjevic University of Kragujevac, Serbia, Medical Faculty
Milan Zaric University of Kragujevac, Medical Faculty
Dusko Avramovic Special Hospital for Internal Diseases, Mladenovac
Nebojsa Arsenijevic Univeristy of Kragujevac, Medical faculty

DOI: https://doi.org/10.5937/sjecr13-2019

Keywords: Superoxides, Cells, Cell Line, Anions,

Abstract

Background/aim: Korbazol is a natural product which has been shown to exert cytotoxic effect on the in vitro survival of leukemic cells. The cytotoxicity and biochemical events induced by Korbazol were investigated in murine cell lines 4T1, B16 and BCL1.

Methods: Cytotoxic activity of the tested compound was assessed by MTT colorimetric assay. The concentration of superoxide anion radical (O₂^.-) and superoxide dismutase (SOD) activity in the samples were determined by spectrophotometric method. The MDA content was determined by TBA assay.

Results: We found that Korbazol induces cell toxicity, increased concentration of lipid peroxidation end product MDA, decrease of superoxide dismutase activity and increase of superoxide anion formation.

Conclusions: Altogether these results suggest that oxidative stress is involved in Korbazol induced cytotoxicity in investigated cell lines.

Keywords: Korbazol; 4T1; B16; BCL1; apoptosis; oxidative stress