PHENOLIC AND FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY OF Daphne blagayana GROWING IN SERBIA
Abstract
Phenolic and flavonoid content, as well as the antioxidant activity of methanol and chloroform extracts from the leaves and twigs of Daphne blagayana were determined. The total phenolic content in the chloroform extract of twigs (90.26±0.69 mg GA/g) of the plant was higher than that of the other extracts (from 76.56±0.89 to 77.45±0.43 mg GA/g). Flavonoids were also more represented in the twigs extracts of D. blagayana. Several different methods were used to determine the antioxidant activity of the tested extracts including total antioxidant capacity, metal chelating activity, hydroxyl radical scavenging activity and inhibitory activity against lipid peroxidation. The highest antioxidant activity was found for the chloroform extract of the plant twigs. A significant correlation between antioxidant activity and phenolic and flavonoid contents was revealed. The results of the present analysis demonstrated, for the first time, that Daphne blagayana leaves and twigs possess high phenolic and flavonoid contents and potential antioxidant activity. This study suggests that Daphne blagayana twigs and leaves may potentially be used as an accessible source of natural antioxidants.
Key words: Daphne blagayana, phenols, flavonoids, antioxidant activity.
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