THE EFFECTS OF VIBROACOUSTICALLY-INDUCED MICROVIBRATIONS ON ARTERIAL BLOOD PRESSURE AND OXIDATIVE STRESS IN RATS
Abstract
Abstract
Vibroacoustics is a scientific field which has been intensively developed for the last thirty years; it uses the capabilities of sound (infrasound, ultrasound, noise and music) to induce vibrations, which, like a sound, may have useful or harmful effects. The aim of this paper was to examine the effects of vibroacoustically-induced microvibrations on arterial blood pressure and markers of oxidative stress in the rat blood. The experiments were done on Wistar male rats, 180-200 g body mass, divided into control and experimental groups (6 rats in each). In the experimental group, microvibrations were induced using vibroacoustic apparatus (the so-called phoning, Vitafon, St. Petersburg, Russian Federation), with total of 60 min phoning time in 4 different application regimes, (5-minute-stabilization time, each 10-minute phoning regime each with 5-minute-breaks between single regimes, 30Hz-18000 kHz frequency rates and 2.8 μm-12.3 μm microwave amplitudes, kidney and liver phoning). After the completion of phoning experimental protocol, the animals were sacrificed and the values of oxidative stress markers were analyzed in blood samples (O2-, H2O2, nitrites, lipid peroxidation index, superoxide dismutase, catalase, glutathione) and compared with the values of markers in the control group. The analysis of values of systolic arterial pressure after acute application of different regimes of vibroacoustic microvibrations showed statistically significant decrease of systolic arterial pressure during the administration of the second regime in comparison to the control group. Systolic arterial pressure was found to return to nearly initial value after the application of vibrocoustic microvibrations in the third and fourth regimes. The analysis of values of diastolic arterial pressure after acute administration of vibroacoustic microvibrations in four different regimes did not show statistically significant decrease of diastolic arterial pressure in any regimes, although the pressure tended to decrease in the first and second regimes. Diastolic arterial pressure was found to return to nearly initial value after the administration of vibrocoustic microvibrations in the third and fourth regimes. The obtained results showed the occurrence of high statistically significant differences in catalase, while no statistically significant differences were found in relation to other analyzed parameters, although positive trend was observed after the phoning. Further research is necessary in order to define the applicative effects of low in regard to high frequencies of the vibroacoustically-induced microvibrations and physiological, i.e. possible therapeutic significance of the registered effects.
Key words: vibroacoustics, arterial blood pressure, oxidative stress, microvibrations
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