ISPITIVANJE IN VITRO OSETLJIVOSTI BORRELIA AFZELII, UZROČNIKA LAJMSKE BORELIOZE, NA SUPERNATANTE LACTIPLANTIBACILLUS PLANTARUM I SACCHAROMYCES BOULARDII

Kristina  Jevremović; Katarina Kilibarda; Dragana  Božić; Jelena Antić Stanković; Biljana  Nikolić; Snežana  Tomanović; Gorana Veinović

doi:10.5937/smclk6-58916

Kristina Jevremović Univerzitet u Beogradu, Institut za medicinska istraživanja, Institut od nacionalnog značaja za Republiku Srbiju
Katarina Kilibarda Univerzitet u Beogradu, Vinča - Institut za nuklearne nauke, Nacionalni institut Republike Srbije
Dragana Božić Univerzitet u Beogradu, Farmaceutski fakultet
Jelena Antić Stanković Univerzitet u Beogradu, Farmaceutski fakultet
Biljana Nikolić Univerzitet u Beogradu, Biološki fakultet
Snežana Tomanović Univerzitet u Beogradu, Institut za medicinska istraživanja, Institut od nacionalnog značaja za Republiku Srbiju
Gorana Veinović Univerzitet u Beogradu, Institut za medicinska istraživanja, Institut od nacionalnog značaja za Republiku Srbiju

DOI: https://doi.org/10.5937/smclk6-58916

Ključne reči: Lajmska borelioza, Borrelia afzelii, osetljivost, in vitro, probiotski mikroorganizmi, minimalne inhibitorne koncentracije

Sažetak

Uvod: Lajmska borelioza (LB) je multisistemsko oboljenje koje uzrokuju bakterije iz kompleksa Borrelia burgdorferi sensu lato, a prenose je ubodom krpelji roda Ixodes. Terapija antibioticima zahteva dugotrajno lečenje, dok trenutno na tržištu ne postoji topikalni proizvod za profilaktičku primenu nakon uboda krpelja koji bi sprečio lokalnu infekciju i dalju diseminaciju patogena u sistemsku cirkulaciju.

Cilj rada: Cilj studije bio je da se in vitro ispita uticaj supernatanata probiotskih mikroorganizama Saccharomyces boulardii i Lactiplantibacillus plantarum i njihovih mešavina na rast i pokretljivost Borrelia afzelii.

Materijali i metode: Korišćen je soj B. afzelii, prethodno izolovan iz krpelja vrste Ixodes ricinus, koji je umnožen do koncentracije 1-2x10⁶ borelija/mL u Barbour-Stoenner-Kelly-H hranljivoj podlozi. Za ispitivanje osetljivosti testiranog soja na probiotske mikroorganizme korišćena je modifikovana bujon mikrodiluciona metoda za borelije. Minimalne inhibitorne koncentracije (MIK) određene su posmatranjem broja i pokretljivosti borelija nakon 72 sata inkubacije pod anaerobnim uslovima u prisustvu supernatanata, a u poređenju sa pozitivnom kontrolom – netretiranom kulturom B. afzelii.

Rezultati: MIK je definisana kao najniža koncentracija testiranog supernatanta pri kojoj borelije nisu bile pokretne a njihov broj je bio smanjen u odnosu na pozitivnu kontrolu. Definisane su sledeće MIK vrednosti: medijana 12,5% za S. boulardii i 6% za L. plantarum, dok je najniža MIK vrednost (medijana 0,8%) definisana za mešavinu supernatanta probiotskih mikroorganizama.

Zaključak: Supernatanti S. boulardii i L. plantarum pokazali su antimikrobno dejstvo na B. afzelii, dok je mešavina ovih supernatanata pokazala efektivniji inhibitorni efekat na rast i pokretljivost borelija. Dobijeni rezultati daju nam osnovu za dalja istraživanja molekularnih mehanizama inhibitornog dejstva probiotskih mikroorganizama na rast borelija.

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