Mezenhimske matične ćelije iz periapeksnih lezija moduliraju produkciju citokina od strane lokalnih imunskih ćelija

  • Milan Marković Military Medical Academy, Institute for Medical Research, Clinic for Stomatology, Belgrade, Serbia
  • Sergej Tomić University of Defence in Belgrade, Medical Faculty of the Military Medical Academy, Belgrade, Serbia
  • Jelena Djokić Military Medical Academy, Institute for Medical Research, Clinic for Stomatology, Belgrade, Serbia
  • Dušan Mihajlović University of Defence in Belgrade, Medical Faculty of the Military Medical Academy, Belgrade, Serbia
  • Dragana Vučević Military Medical Academy, Institute for Medical Research, Clinic for Stomatology, Belgrade, Serbia
  • Dragan Gazivoda Military Medical Academy, Department for Oral Surgery, Belgrade, Serbia
  • Miloš Duka Military Medical Academy, Department for Oral Surgery, Belgrade, Serbia
  • Miodrag Čolić INEP – Institute for Nuclear Energy, University of Belgrade, Serbia
DOI: https://doi.org/10.2298/VSP160901272M
Ključne reči: periapikalne bolesti;, ćelije, matične;, apsces, periapikalni;, zapaljenje; citokini;, fenotip;, citometrija, protočna.

Sažetak


Uvod/Cilj. Pokazano je da mezenhimske matične ćelije (engl. mesenchymal stem cells – MSC) suprimiraju imunske i inflamacijske reakcije. Međutim, nije poznato da li MSC iz inflamacijom zahvaćenih tkiva, kao što su periapeksne lezije (engl. periapical lesions – PLs) ispoljavaju slične efekte. Upravo je ovo pitanje razmatrano u našem radu čiji cilj je bio da se ispita sposobnost PL-MSC da moduliraju produkciju citokina od strane imunskih ćelija lokalno. Metode. MSC su izolovane iz asimptomatskih (engl. asymptomatic – as) i simptomatskih (engl. symptomatic – sy) PL zuba. Njihov fenotip je određen metodom protočne citofluorimetrije na osnovu detekcije površinskih markera specifičnih za MSC. Antiinflamacijska i imunomodulacijska svojstva PL-MSC ispitivana su merenjem produkcije citokina u kokulturi sa mononuklearnim ćelijama (engl. mononuclear cells – MNCs) izolovanim iz asPLs i syPLs. Nivo produkovanih citokina u supernatantima određivan je ELISA metodom. Rezultati. Obe PL-MSC linije karakterisao je fenotip tipičan za druge tipove MSC u kome je dominirala ekspresija CD29, CD44, CD90, CD105 i CD166 markera. Izolovane PL-MSC linije su, nezavisno od fenotipske sličnosti, ispoljile isti modulacijski efekat na produkciju citokina, ali je odgovor asPL-MNCs i syPL-MNCs bio različit, uprkos sličnom sastavu MNCs u oba tipa lezija. Oba tipa MSC linija inhibirala su produkciju proinflamacijskih citokina kao što su interleukin 1β (IL-1β) i faktor nekroze tumora α (TNF-α). Međutim, produkcija IL-8 bila je snižena jedino u kokulturi sa syPL-MNCs. PL-MSC linije su takođe modulirale produkciju imunoregulacijskih citokina. Produkcija transformišućeg faktora rasta β (TGF-β) bila je povećana u kokulturama sa oba tipa MNC, i asPL-MNCs i syPL-MNCs, a nivo IL-10 bio je povećan jedino u kokulturi sa asPL-MNCs. Zaključak. Naši rezultati pokazuju da PL-MSC doprinose smanjenju lokalne inflamacije i imunskog odgovora, ali je ovaj efekat verovatno manje efikasan u toku egzacerbacije inflamacije u PLs.

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Objavljeno
2020/12/01
Broj časopisa
Vol. 75 Br. 5 (2018): Vojnosanit Pregl 2018; May Vol. 75 (No. 5): p. 435–540.
Rubrika
Originalni članak