Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract

Tatjana Ćebović; Dunja Jakovljević; Zoran Maksimović; Snezana Djordjevic; Sanja Jakovljević; Dragana Četojević-Simin

doi:10.2298/VSP170713084C

Tatjana Ćebović University of Novi Sad, Faculty of Medicine, Biochemistry Department, Novi Sad, Serbia
Dunja Jakovljević University of Novi Sad, Faculty of Medicine, Biochemistry Department, Novi Sad, Serbia
Zoran Maksimović University of Belgrade, Faculty of Pharmacy, Institute of Pharmacognosy, Belgrade, Serbia
Snezana Djordjevic Military Medical Academy, National Poison Control Center, Belgrade, Serbia
Sanja Jakovljević University of Novi Sad, Faculty of Medicine, Biochemistry Department, Novi Sad, Serbia
Dragana Četojević-Simin University of Novi Sad, Faculty of Medicine, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia

DOI: https://doi.org/10.2298/VSP170713084C

Keywords: antioksidansi, lipidi, peroksidacija, fitoterapija, biljke, lekovite, polygonaceae

Abstract

Uvod/Cilj: Rumex crispus (štavelj) je višegodišnja zeljasta biljka, koja se smatra bogatim izvorom biološki aktivnih molekula sa antioksidantnom i citotoksičnom aktivnošću. Cilj rada bio je procena antioksidantnog kapaciteta i ispitivanje antitumorske aktivnosti vodenog ekstrakta ploda štavelja. Metode. Antioksidantna aktivnost vodenog ekstrakta ploda štavelja procenjena je na osnovu in vitro testova: ferric-reducing antioxidant power (FRAP), sposobnosti ekstrakta da neutrališe slobodne radikale NO•, OH• i 2,2-difentil-1-pikril hidrazil (DPPH) i utiče na lipidnu peroksidaciju u lipozomima. Citotoksičnost ispitivanog ekstrakta je određena in vitro na tumorskim ćelijskim linijama: humani karcinom cerviksa (HeLa), adenokarcinom (HT-29) i adenokarcinom dojke (MCF7). Rezultati. Testirani ekstrakt pokazao je potencijalnu antioksidantnu aktivnost manifestovanu velikom moći u neutralizaciji slobodnih radikala, kao i sposobnost da smanji lipidnu peroksidaciju u lipozomima. Ustanovljena je tkivno-selektivnu citotoksičnost ekstrakta ploda štavelja in vitro. Najizraženija antitumorska aktivnost primećena je prema HeLa i MCF7 ćelijskim linijama. Zaključak. Vodeni ekstrakt ploda štavelja ima potencijalnu antioksidantnu i citotoksičnu aktivnost, sa nekrozom kao glavnim mehanizmom indukovane ćelijske smrti. Za dalja istraživanja, preporučuju se i druge metode ekstrakcije ploda štavelja.

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