Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition

Marko  Magić; Božana Čolović; Vukoman Jokanović; Saša Vasilijić; Milan Marković; Dragana Vučević; Rebeka Rudolf; Snježana Čolič; Miodrag Čolić

doi:10.2298/VSP170809097M

Marko Magić University of Belgrade, School of Dentistry, Department of Oral Surgery, Belgrade, Serbia
Božana Čolović University of Belgrade, Vinča Institute of Nuclear Sciences, Belgrade, Serbia
Vukoman Jokanović University of Belgrade, Vinča Institute of Nuclear Sciences, Belgrade, Serbia
Saša Vasilijić Military Medical Academy, Institute for Medical Research, Belgrade, Serbia; University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade
Milan Marković Military Medical Academy, Institute for Medical Research, Belgrade, Serbia
Dragana Vučević Military Medical Academy, Institute for Medical Research, Belgrade, Serbia
Rebeka Rudolf University of Maribor, Faculty of Mechanical Engineering, Maribor, Slovenia
Snježana Čolič University of Belgrade, School of Dentistry, Department of Oral Surgery, Belgrade, Serbia
Miodrag Čolić University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade Serbia

DOI: https://doi.org/10.2298/VSP170809097M

Keywords: dental alloys;, titanium;, materials testing;, hydroxyapatites.

Abstract

Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo.

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