The effects of acutely and subchronically applied DL-methionine  on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

Žarko Mićović; Sanja Kostić; Slavica Mutavdžin; Aleksa Andrejević; Aleksandra Stamenković; Mirjana Čolović; Danijela Krstić; Marko Djurić; Dragan Hrnčić; Vladimir Živković; Vladimir Jakovljević; Dragan Djurić

doi:10.2298/VSP171213055M

Žarko Mićović Ministry of Defence, Military Health Department, Belgrade, Serbia
Sanja Kostić University of Belgrade, Faculty of Medicine, Institute of Medical Physiology „Richard Burian“, Belgrade, Serbia
Slavica Mutavdžin University of Belgrade, Faculty of Medicine, Institute of Medical Physiology „Richard Burian“, Belgrade, Serbia
Aleksa Andrejević University of Priština – Kosovska Mitrovica, Faculty of Medical Medicine, Clinic of Gynecology and Obstetrics, Kosovska Mitrovica, Serbia
Aleksandra Stamenković University of Manitoba, St. Boniface Hospital Research Center, §Institute of Cardiovascular Sciences, Winnipeg, Canada
Mirjana Čolović University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Danijela Krstić University of Belgrade, Faculty of Medicine, Institute of Medical Chemistry, Belgrade, Serbia
Marko Djurić University Clinical Hospital Center „Dr Dragiša Mišović – Dedinje“, Department of Anesthesiology, Reanimatology and Intensive Care Medicine, Belgrade, Serbia
Dragan Hrnčić University of Belgrade, Faculty of Medicine, Institute of Medical Physiology „Richard Burian“, Belgrade, Serbia
Vladimir Živković University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
Vladimir Jakovljević University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
Dragan Djurić University of Belgrade, Faculty of Medicine, Institute of Medical Physiology „Richard Burian“, Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP171213055M

Keywords: oxidative stress, methionine, homocysteine, rats, plasma, enzymes, cholinesterases

Abstract

Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.

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