Association of PAX3 and TMTC2 genes polymorphism with the face morphology changes after excision of skin tumors

Saša  Milićević; Zvonko Magić; Gordana Šupić; Aleksandar Jevtić; Stevo  Jovandić; Nenad Stepić

doi:10.2298/VSP180613128M

Saša Milićević Military Medical Academy, Clinic for Plastic Surgery and Burns, Belgrade, Serbia
Zvonko Magić Military Medical Academy, Institute of Medical Research, Belgrade, Serbia
Gordana Šupić Military Medical Academy, Institute of Medical Research, Belgrade, Serbia
Aleksandar Jevtić Military Medical Academy, Clinic for Orthopedics and Traumatology, Belgrade, Serbia
Stevo Jovandić Military Medical Academy, Institute of Medical Research, Belgrade, Serbia
Nenad Stepić Military Medical Academy, Clinic for Plastic Surgery and Burns, Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP180613128M

Keywords: polymorphism, genetic, face, skin neoplasms, surgical procedures, operative

Abstract

Background/Aim. The group of genes, known as PAX (paired box), has a great role in organogenesis, as well as in maintaining the normal function of certain cells after the birth. In addition to these genes, the impact on the organogenesis, at the cellular level, has a transmembrane tetratricopeptid group of genes (TMTC). The term polymorphism in the human genome implies variations in the hereditary basis that occur in human populations, the presence of two or more different alleles of one genome in the population. The aim of the work was to determine whether there is an association of PAX3 and TMTC2 genes polymorphism with changes of the face morphology after skin tumor excision and direct suture closure. Methods. The study included 130 patients of both sexes, older than 50 years, with the medical indication for the elliptical surgical excision of the skin tumor. DNA was isolated from 5 mL of peripheral blood. Gene polymorphisms were analyzed with pre-designed single nucleotide polymorphisms (SNP) assays, by allelic discrimination method on REAL-TIME apparatus. The patients were subjected to a laser scanning preoperatively, and 7 and 90 days postoperatively, in order to obtain x, y and z coordinates of 5 cephalometric points on the face, which determined the shape of the medial cheek region. The shape of the medial cheek region, as well as the coordinates of 5 cepahlometric points, were compared among genotypes of both genes preoperatively, as well as 7 days and 90 days postoperatively. Results. A statistically significant difference in the shape of the medial cheek region between wild-type and mutant of PAX3 gene was found preoperatively, while the statistically significant difference in the shape of the medial cheek region was not found between wild-type and heterozygote, nor between wild-type and heterozygote and mutant of PAX3 gene, nor among genotypes of TMTC2 gene. Seven days and 90 days postoperatively, there were no statistically significant differences in the shape of the examined region among genotypes of both genes. Conclusion. Polymorphisms of PAX3 and TMTC2 genes are not associated with the change in the face morphology after the skin tumor excision and direct suture closure of the defect.

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