Three obstetric factors should be considered in umbilical cord blood donor selection
Abstract
Background/Aim. The umbilical cord blood (UCB) volume and hematopoietic stem cells count are used as indicators for hematopoietic potential of UBC units. These indicators are affected by a collection method and obstetric factors. It was established that birth weight and placental weight affect the volume and hematopoietic stem cells count in UCB units. The influence of other obstetric factors is less clear. The aim of this study was to investigate the impact of obstetric factors on hematopoietic potential of UCB units. Methods. The study involved 103 consecutive UCB units collected during 2013. Relationship of UCB volume, total nucleated cells, CD34+ cells and Colony Forming Unit-Granulocyte Monocyte count with maternal and neonatal characteristics was retrospectively analyzed. Results. It was shown that birth weight, placental weight and umbilical cord length ≥ 31 cm significantly increased the volume of collected samples, total nucleated cells, CD34+ cells and Colony Forming Unit-Granulocyte Monocyte count. Gestational age between 38−40 weeks increased significantly all umbilical factors (volume, total nucleated cells, CD34+ cells, and Colony Forming Unit-Granulocyte Monocyte count). Gender did not have an influence on quality of UCB units except on total nucleated cells and CD34+ cells count. Other obstetric factors did not affect significantly the quality of UCB units. Conclusion. Our study confirmed that birth weight, placenta weight, length of the umbilical cord and gestational age independently influenced the UCB unit volume, and absolute count of nuclear cells and hematopoietic stem cells. Due to a positive correlation between birth weight and placental weight, only birth weight, umbilical cord length and gestational age should be standard parameters in procedure of donor selection.
References
Gluckman E, Broxmayer HE, Auerbach AD. Hematopoetic re-construction in a patient with Fanconi anemia by means of umbilical-cord from HLA-identical sibling. N Engl J Med 1989; 321(17): 1174–8.
Benito AI, Diaz MA, Gonzalez-Vicent M, Sevilla J, Madero L. Hematopoietic stem cell transplantation using umbilical cord blood progenitors: review of current clinical results. Bone Marrow Transplant 2004; 33(7): 675–90.
Warwick R, Armitage S. Cord blood banking. Best Pract Res Clin Obstet Gynaecol 2004; 18(6): 995‒1011.
Harris DT, Schumacher MJ, Rychlik S, Booth A, Acevedo A, Ru-binstein P, et al. Collection, separation and cryopreservation of umbilical cord blood in transplantation. Bone Marrow Trans-plant 1994; 13(2): 135–43.
Rogers I, Sutherland, Holt D, Macpate F, Lains A, Hollowell S, et al. Human UC-blood banking: impact of blood volume, cell separation and cryopreservation on leukocyte and CD34(+) cell recovery. Cytotherapy 2001; 3(4): 269‒76.
Škorić D, Balint B, Petakov M, Sinđić M, Rodić P. Collection strategies and cryopreservation of umbilical cord blood. Trans-fus Med 2007; 17(2): 107‒13.
Ballen KK, Wilson M, Wuu J, Ceredona AM, Hsieh C, Stewart FM, et al. Bigger is better: maternal and neonatal predictors of hematopoietic potential of umbilical cord blood units. Bone Marrow Transplant 2001; 27(1): 7‒14.
Sparrow RL, Cauchi JA, Ramadi LT, Waugh CM, Kirkland MA. Influence of mode of birth and collection on WBC yields of umbilical cord blood units. Transfusion 2002; 42(2): 210‒15.
Jones J, Stevens CE, Rubinstein P, Robertazzi RR, Kerr A, Cabbad MF. Obstetric predictors of placental/umbilical cord blood volume for transplantation. Am J Obstet Gynecol 2003; 188(2): 503‒9.
Askari S, Miller J, Chrysler G, McCullough J. Impact of donor- and collection-related variables on product quality in ex utero cord blood banking. Transfusion 2005; 45(2): 189‒94.
Keeney M, Chin-Yee I, Weir K, Popma J, Nayar R, Sutherland DR. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering. Cytometry 1998; 34(2): 61‒70.
Solves P, Perales A, Moraga R, Saucedo E, Soler MA, Monleon J. Maternal, neonatal and collection factors influencing the haematopoietic content of cord blood units. Acta Haematol 2005; 113(4): 241‒6.
Mancinelli F, Tamburini A, Spagnoli A, Malerba C, Suppo G, Las-orella R, et al. Optimizing umbilical cord blood collection: im-pact of obstetric factors versus quality of cord blood units. Transplant Proc 2006; 38(4): 1174‒6.
George TJ, Sugrue MW, George SN, Wingard JR. Factors associ-ated with parameters of engraftment potential of umbilical cord blood. Transfusion 2006; 46(10): 1803‒12.
Juutistenaho S, Eskola M, Sainio S, Aranko K, Kekomäki R. Asso-ciation of stress-related perinatal factors and cord blood unit hematopoietic progenitors is dependent on delivery mode. Transfusion 2010; 50(3): 663‒71.
Keersmaekers CL, Mason BA, Keersmaekers J, Ponzini M, Mlynarek RA. Factors affecting umbilical cord blood stem cell suitability for transplantation in an in utero collection pro-gram. Transfusion 2014; 54(3): 545‒9.
Nunes RD, Zandavalli FM. Association between maternal and fetal factors and quality of cord blood as a source of stem cells. Rev Bras Hematol Hemoter. 2015; 37: 38–42.
Urciuoli P, Passeri S, Ceccarelli F, Luchetti B, Paolicchi A, Lapi S, et al. Pre-birth selection of umbilical cord blood donors. Blood Transfus 2010; 8(1): 36‒43.
Wen SH, Zhao WL, Lin PY, Yang KL. Associations among birth weight, placental weight, gestational period and product quality indicators of umbilical cord blood units. Transfus Apher Sci 2012; 46(1): 39–45.
Hussein AA, Bawadi RM, Tahtamouni LH, Frangoul H, ElKarmi AZ. Feasibility of Collecting Umbilical Cord Blood in Jordan and the Effect of Maternal and Neonatal Factors on Hemato-poietic Stem Cell Content. Mediterr J Hematol Infect Dis 2014; 6(1): e2014019.
Page K, Mendizabal A, Betz-Stablein B, Wease S, Shoulars K, Gen-try T, et al. Optimizing Donor Selection for Public Cord Blood Banking: Influence of Maternal, Infant and Collection Characteristics on Cord Blood Unit Quality. Transfusion 2014; 54(2): 340–52.
Wu, JY, Liao, C, Chen, JS, Xu, ZP, Gu, SL, Wu, SQ, et al. Analysis of maternal and neonatal factors associated with hematopoietic reconstruction potential in umbilical cord blood units. Zhongguo Shi Yan Xue Ye Xue Za Zhi 2010; 18(6): 1535–41. (Chinese)
Bassiouny MR, El-Chennawi F, Mansour AK, Yahia S, Darwish A. ptimal method for collection of umbilical cord blood: an Egyptian trial for a public cord blood bank. Transfusion 2015; 55(6): 1263‒8.
Chandra T, Afreen S, Kumar A, Singh U, Gupta A. Does Umbil-ical Cord Blood-derived CD34+ Cell Concentration Depend on the Weight and Sex of a Full-term Infant? J Pediatr Hema-tol Oncol 2012; 34: 184–7.
Philip J, Kushwaha N, Chatterjee T, Mallhi RS. Optimizing cord blood collections: assessing the role of maternal and neonatal factors. Asian J Transfus Sci 2015; 9(2): 163–7.