Moderate radioprotective role of zeolite in rats

Miloš Pavlović; Mirjana Djukic; Danilo Vojvodić; Milica Ninković; Ivana Stevanović; Ana Djurić; Boban Stanojević

doi:10.2298/VSP190702136P

Miloš Pavlović University of Belgrade, Faculty of Veterinary Medicine, Department of Reproduction, Fertility and Artificial Insemination, Belgrade, Serbia
Mirjana Djukic University of Belgrade, Faculty of Pharmacy, Department of Toxicology, Belgrade, Serbia
Danilo Vojvodić University of Defense, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Milica Ninković University of Defense, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Ivana Stevanović University of Defense, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Ana Djurić University of Belgrade, Faculty of Pharmacy, Department of Toxicology, Belgrade, Serbia
Boban Stanojević University of Belgrade, Institute of Nuclear Sciences “Vinča”, Department for Radiobiology and Molecular Genetics (080), Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP190702136P

Keywords: brain, oxidative stress, plasma, radiation, ionizing, rats, zeolites

Abstract

Background/Aim. Exposure of living organisms to γ-radiation results in the overproduction of free radicals. The aim of the study was to test if the subacute administration of micronized zeolite (MZC) accomplishes radioprotective role based on the evaluation of the status of oxidative stress (OS) in the brain and 8-hydroxyguanosine (8-OH-dG) in the plasma of the rats exposed to the single γ-ray irradiation of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a four-week normal or 5% MZC supplemented diet and afterward exposed to the single γ-ray irradiation of 2 and 10 Gy. Groups of rats were: a) on a normal diet (the control group, and 2Gy and 10Gy groups); b) on 5% MZC supplemented diet (the control group – MZC, MZC + 2Gy, and MZC + 10Gy groups). We measured malondialdehyde (MDA), glutathione (GSH) total, and activity of total superoxide dismutase (tSOD) and manganese superoxide dismutase (MnSOD) in vulnerable brain regions (cerebellum, hippocampus, and cerebral cortex) and 8-OH-dG in plasma. Results. Lower MDA was found in the MZC+2Gy and MZC+10Gy groups compared to the 2Gy and 10Gy groups. Activity od total SOD was higher in the MZC+10Gy group than in the 10Gy one. GSH was the highest in the 10Gy group. Compared to the control group, 8-OH-dG was extremely higher in groups radiated with 10 Gy regardless of a diet, but slightly lower in the MZC+2Gy and 2Gy groups. Conclusion. Subacute MZC pretreatment accomplished partial radioprotective effect in irradiated rats compared to non-irradiated rats, based on suppressed SOD activity at 2 Gy, and reduced brain MDA when exposed to 2 Gy and 10 Gy.

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