The importance of determining lactate dehydrogenase in laboratory and experimental work in oncology

Crabtree HG. Observations on the carbohydrate metabolism of tumours. Biochem J 1929; 23(3): 536–45.

McKeehan WL. Glycolysis, glutaminolysis and cell prolifera-tion. Cell Biol Int Rep 1982; 6(7): 635–50.

Munyon WH, Merchant DJ. The relation between glucose utili-zation, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp Cell Res 1959; 17(3): 490–98.

Chian CF, Wu FP, Tsai CL, Peng CK, Shen CH, Perng WC, et al. Echogenic swirling pattern, carcinoembryonic antigen, and lactate dehydrogenase in the diagnosis of malignant pleural ef-fusion. Sci Rep 2022; 8; 12(1): 4077.

Shimoda M, Tanaka Y, Morimoto K, Yoshiyama T, Yoshimori K, Ohta K. Diagnostic flowchart for tuberculous pleurisy, pleural infection, and malignant pleural effusion. Respir Investig 2024; 62(1): 157–63.

Chantharakhit C, Sujaritvanichpong N. Developing a Prediction Score for the Diagnosis of Malignant Pleural Effusion: MPE Score. Asian Pac J Cancer Prev 2022; 23(1): 25–31.

Jurisic V, Obradovic J, Nikolic N, Javorac J, Perin B, Milasin J. Analyses of P16INK4a gene promoter methylation relative to molecular, demographic and clinical parameters characteristics in non-small cell lung cancer patients: A pilot study. Mol Biol Rep 2023; 50(2): 971–9.

Lin CJ, Chen YC, Chen HH, Wu CJ, Hsu JM. Renal cell carci-noma presenting as a huge simple renal cyst. Med Oncol 2008; 25(1): 104–6.

Parker MF, Conslato SS, Chang AS, Taylor RR, Reed ME, Mayer AR. Chemical analysis of adnexal cyst fluid. Gynecol Oncol 1999; 73(1): 16–20.

Mohan G, Bhide P, Agrawal A, Kaul V, Chaddha U. A practical approach to pseudoexudative pleural effusions. Respir Med 2023; 214: 107279.

Adeva-Andany M, López-Ojén M, Funcasta- Calderón R, Amenei-ros-Rodríguez EA, Donapetry-García C, Vila-Altesor M, et al. Comprehensive review on lactate metabolism in human health. Mitochondrion 2014; 17: 76–100.

Samanta S, Sharma A, Das B, Mallick AK, Kumar A. Signifi-cance of Total Protein, Albumin, Globulin, Serum Effusion Albumin Gradient and LDH in the Differential Diagnosis of Pleural Effusion Secondary to Tuberculosis and Cancer. J Clin Diagn Res 2016; 10(8): BC14–8.

Ouyang QC, Wang PH. The variation of the serum level of lac-tic dehydrogenace in 105 patients with non-Hodgkin’s and its clinical significance. J Pract Oncol 2001; 16: 111–3.

Konjević G, Jurisić V, Jakovljević B, Spuzić I. Lactate dehydrogen-ase (LDH) in peripheral blood lymphocytes (PBL) of patients with solid tumors. Glas Srp Akad Nauka Med 2002; 47: 137–47.

Konjević G, Jurisić V, Spuzić I. Association of NK cell dysfunc-tion with changes in LDH characteristics of peripheral blood lymphocytes (PBL) in breast cancer patients. Breast Cancer Res Treat 2001; 66(3): 255–63.

Jurisić V, Konjević G, Banićević B, Duricić B, Spuzić I. Different alterations in lactate dehydrogenase (LDH) activity and pro-file of peripheral blood mononuclear cells in Hodgkin’s and non-Hodgkin’s lymphomas. Eur J Haematol 2000; 64(4): 259–66.

Hanson PJ, Parsons S. Metabolism and transport of glutamine and glucose in vascularly perfused small intestine rat. Biochem J 1997; 166(3): 509–19.

Stokkel MP, van Eck-Smit BL, Zwinderman AH, Willems LN, Pauwels EK. Pretreatment serum LDH as additional staging pa-rameter in small-cell lung carcinoma. Neth J Med 1998; 52(2): 65–70.

Hailemariam TS, Mehdi M, Kinde S, Seifu D, Edao A. BCR-ABL Transcript Level as Compared to LDH and Uric Acid Among Chronic Myeloid Leukemic Patients. Recent Pat Anticancer Drug Discov 2021; 16(3): 445–55.

Samlowski W. The Effect of Non-Overlapping Somatic Muta-tions in BRAF, NRAS, NF1, or CKIT on the Incidence and Outcome of Brain Metastases during Immune Checkpoint In-hibitor Therapy of Metastatic Melanoma. Cancers (Basel) 2024; 16(3): 594.

Tiainen S, Nurmela V, Selander T, Turunen P, Pasonen-Seppänen S, Kettunen T, et al. A practical prognostic peripheral blood-based risk model for the evaluation of the likelihood of a response and survival of metastatic cancer patients treated with im-mune checkpoint inhibitors. BMC Cancer 2023; 23(1): 1186.

Di Gioia D, Blankenburg I, Nagel D, Heinemann V, Stieber P. Tumor markers in the early detection of tumor recurrence in breast cancer patients: CA 125, CYFRA 21-1, HER2 shed an-tigen, LDH and CRP in combination with CEA and CA 15-3. Clin Chim Acta 2016; 461: 1–7.

Jurisić V, Konjević G, Jancić-Nedeljkov R, Sretenović M, Banicević B, Colović M, et al. The comparison of spontaneous LDH release activity from cultured PBMC with sera LDH activity in non-Hodgkin’s lymphoma patients. Med Oncol 2004; 21(2): 179–85.

Lu R, Jiang M, Chen Z, Xu X, Hu H, Zhao X, et al. Lactate de-hydrogenase 5 expression in Non-Hodgkin lymphoma is asso-ciated with the induced hypoxia regulated protein and poor prognosis. PLoS One 2013; 8(9): e74853.

Halvorsen CP, Olson L, Araújo AC, Karlsson M, Nguyễn TT, Khu DT, et al. A rapid smartphone-based lactate dehydrogenase test for neonatal diagnostics at the point of care. Sci Rep 2019; 9(1): 9301.

DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB. The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab 2008; 7(1): 11–20.

Christen S, Sauer U. Intracellular characterization of aerobic glucose metabolism in seven yeast species by 13C flux analysis and metabolomics. FEMS Yeast Res 2011; 11(3): 263–72.

Semenza GL, Roth PH, Fang HM, Wang GL. Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1. J Biol Chem 1994; 269(38): 23757–63.

Radenkovic S, Milosevic Z, Konjevic G, Karadzic K, Rovcanin B, Buta M, et al. Lactate dehydrogenase, catalase, and superoxide dis-mutase in tumor tissue of breast cancer patients in respect to mammographic findings. Cell Biochem Biophys 2013; 66(2): 287–95.

White BE, Liu Y, Hakonarson H, Buono RJ. RNA Sequencing in Hypoxia-Adapted T98G Glioblastoma Cells Provides Sup-portive Evidence for IRE1 as a Potential Therapeutic Target. Genes (Basel) 2023; 14(4): 841.

Pestereva N, Ivleva I, Zubov A, Tikhomirova M, Karpenko M. m-Calpain is released from striatal synaptosomes. Int J Neurosci 2023; 133(2): 215–21.

Ferrer IM, Valadez H, Estala L, Gomez FA. Paper microfluid-ic-based enzyme catalyzed double microreactor. Electrophore-sis 2014; 35(16): 2417–9.

Koukourakis MI, Kontomanolis E, Giatromanolaki A, Sivridis E, Liberis V. Serum and tissue LDH levels in patients with breast/gynaecological cancer and benign diseases. Gynecol Obstet Invest 2009; 67(3): 162–8.

Jurisic V, Terzic T, Pavlovic S, Colovic N, Colovic M. Elevated TNF-alpha and LDH without parathormone disturbance is as-sociated with diffuse osteolytic lesions in leukemic transfor-mation of myelofibrosis. Pathol Res Pract 2008; 204(2): 129–32.

Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, et al. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature 2009; 462(7274): 739–44.

Koukourakis MI, Giatromanolaki A, Harris AL, Sivridis E. Comparison of metabolic pathways between cancer cells and stromal cells in colorectal carcinomas: a metabolic survival role for tumor-associated stroma. Cancer Res 2006; 66(2): 632–7.

Marsin AS, Bertrand L, Rider MH, Deprez J, Beauloye C, Vincent MF, et al. Phosphorylation and activation of heart PFK-2 by AMPK has a role in the stimulation of glycolysis during is-chaemia. Curr Biol 2000; 10(20): 1247–55.

Jurisić V, Colović M. Correlation of sera TNF-alpha with per-centage of bone marrow plasma cells, LDH, beta2-microglobulin, and clinical stage in multiple myeloma. Med Oncol 2002; 19(3): 133–9.

Kim JW, Tchernyshyov I, Semenza GL, Dang CV. HIF-1-mediated expression of pyruvate dehydrogenase kinase: a met-abolic switch required for cellular adaptation to hypoxia. Cell Metab 2006; 3(3): 177–85.

Fantin VR, St-Pierre J, Leder P. Attenuation of LDH-A expres-sion uncovers a link between glycolysis, mitochondrial physi-ology, and tumor maintenance. Cancer Cell 2006; 9(6): 425–34. Erratum in: Cancer Cell 2006; 10(2): 172.

Kolev Y, Uetake H, Takagi Y, Sugihara K. Lactate dehydrogen-ase-5 (LDH-5) expression in human gastric cancer: association with hypoxia-inducible factor (HIF-1alpha) pathway, angio-genic factors production and poor prognosis. Ann Surg Oncol 2008; 15(8): 2336–44.

Gottlob K, Majewski N, Kennedy S, Kandel E, Robey RB, Hay N. Inhibition of early apoptotic events by Akt/PKB is dependent on the first committed step of glycolysis and mitochondrial hexokinase. Genes Dev 2001; 15(11): 1406–18.

Tataranni T, Agriesti F, Pacelli C, Ruggieri V, Laurenzana I, Mazzoccoli C, et al. Dichloroacetate Affects Mitochondrial Function and Stemness-Associated Properties in Pancreatic Cancer Cell Lines. Cells 2019; 8(5): 478.

Cakici C, Daylan B, Unluer RS, Emekli-Alturfan E, Ayla S, Gozel HE, et al. LDH-A Inhibitor as a Remedy to Potentiate the Anticancer Effect of Docetaxel in Prostate Cancer. J Can-cer 2024; 15(3): 590–602.

Xie J, Wang BS, Yu DH, Lu Q, Ma J, Qi H, et al. Dichloroace-tate shifts the metabolism from glycolysis to glucose oxidation and exhibits synergistic growth inhibition with cisplatin in HeLa cells. Int J Oncol 2011; 38(2): 409–17.

Zhu J, Zheng Y, Zhang H, Sun H. Targeting cancer cell metabo-lism: The combination of metformin and 2-Deoxyglucose reg-ulates apoptosis in ovarian cancer cells via p38 MAPK/JNK signaling pathway. Am J Transl Res 2016; 8(11): 4812–21.

Jurisic V, Kraguljac N, Konjevic G, Spuzic I. TNF-alpha induced changes in cell membrane antigen expression on K-562 cells associated with increased lactate dehydrogenase (LDH) re-lease. Neoplasma 2005; 52(1): 25–31.

Jurisic V. Estimation of cell membrane alteration after drug treatment by LDH release. Blood 2003; 101(7): 2894.

Jurisic V, Srdic-Rajic T, Konjevic G, Bogdanovic G, Colic M. TNF-α induced apoptosis is accompanied with rapid CD30 and slow-er CD45 shedding from K-562 cells. J Membr Biol 2011; 239(3): 115–22.

Tsujimoto Y. Apoptosis and necrosis: intracellular ATP level as a determinant for cell death modes. Cell Death Differ 1997; 4(6): 429–34.

Jurisic V, Bumbasirevic V, Konjevic G, Djuricic B, Spuzic I. TNF-alpha induces changes in LDH isotype profile following trig-gering of apoptosis in PBL of non-Hodgkin’s lymphomas. Ann Hematol 2004; 83(2): 84–91.

Jurisic V, Radenkovic S, Konjevic G. The Actual Role of LDH as Tumor Marker, Biochemical and Clinical Aspects. Adv Exp Med Biol 2015; 867: 115–24.

Jurisic V, Bogdanovic G, Kojic V, Jakimov D, Srdic T. Effect of TNF-alpha on Raji cells at different cellular levels estimated by various methods. Ann Hematol 2006; 85(2): 86–94.

Jurisić V, Spuzić I, Konjević G. A comparison of the NK cell cy-totoxicity with effects of TNF-alpha against K-562 cells, de-termined by LDH release assay. Cancer Lett 1999; 138(1–2): 67–72.

Suresh V, Senthilkumar N, Thangam R, Rajkumar M, Anba-zhagan C, Rengasamy R, et al. Separation, purification and pre-liminary characterization of sulfated polysaccharides from Sar-gassum plagiophyllum and its in vitro anticancer and antioxi-dant activity. Process Biochemistry 2013; 48(2): 364–73.

Živanović M, Gazdić Janković M, Ramović Hamzagić A, Virijević K, Milivojević N, Pecić K, et al. Combined Biological and Numerical Modeling Approach for Better Understanding of the Cancer Viability and Apoptosis. Pharmaceutics 2023; 15(6): 1628.

Li K, Kang H, Wang Y, Hai T, Rong G, Sun H. Letrozole-induced functional changes in carcinoma-associated fibroblasts and their influence on breast cancer cell biology. Med Oncol 2016; 33(7): 64.

Goliwas KF, Richter JR, Pruitt HC, Araysi LM, Anderson NR, Samant RS et al. Methods to evaluate cell growth, viability, and response to treatment in a tissue engineered breast cancer model. Sci Rep 2017; 7(1): 14167.

Sakuraia T, Wakimotoa N, Yamadaa M, Shimamura S, Motoyoshi S. Effect of macrophage colony-stimulating factor on mouse NK 1.1+ cell activity in vivo. Int J Immunopharmacol 1998; 20(8): 401–13.

Matilainen L, Toropainen M, Vihola H, Hirvonen J, Järvinen T, Jarho P, et al. In vitro toxicity and permeation of cyclodextrins in Calu-3 cells. J Control Release 2008; 126(1): 10–6.

Smruthi MR, Nallamuthu I, Singsit D, Anand T. Toxicological evaluation of PLA/PVA-naringenin nanoparticles: In vitro and in vivo studies. Open Nano 2022; 7: 100061.

Karbalaee R, Mehdizadeh S, Ghaleh HEG, Izadi M, Kondori BJ, Dorostkar R, et al. The Effects of Mesenchymal Stem Cells Loaded with Oncolytic Coxsackievirus A21 on Mouse Models of Colorectal Cancer. Curr Cancer Drug Targets 2024; 24(4): 967–74.

Zhang F, Li H, Liu C, Fang K, Jiang Y, Wu M, et al. Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Ex-tract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles. Front Biosci (Landmark Ed) 2022; 27(8): 229.

Feuerecker B, Michalik M, Hundshammer C, Schwaiger M, Bruchertseifer F, Morgenstern A, et al. Assessment of 213Bi-anti-EGFR MAb treatment efficacy in malignant cancer cells with [1-13C] pyruvate and [18F]FDG. Sci Rep 2019; 9(1): 8294.

Michl J, Park KC, Swietach P. Evidence-based guidelines for controlling pH in mammalian live-cell culture systems. Com-mun Biol 2019; 2: 144.