Ultrastrukturne promene peritoneuma na modelu peritoneumske dijalize kod zečeva

  • Nataša Jovanović Clinic of Nephrology, Clinical Center of Serbia, Belgrade, Serbia
  • Snežana Žunić-Božinovski Institute of Pathophysiology, Faculty of Medicine, University of Belgrade, Serbia
  • Dušan Trpinac Institute of Histology and Embriology, University of Belgrade, School of Medicine
  • Željko Laušević Institute for Digestive Diseases,University of Belgrade, School of Medicine
  • Slobodan Krstić Institute for Digestive Diseases,University of Belgrade, School of Medicine
  • Dejan Oprić Institute of Pathology, University of Belgrade, School of Medicine
  • Jasna Trbojević-Stanković Department of Hemodialysis and Nephrology, Clinical Hospital Center „Dr Dragisa Misovic“, Belgrade, Serbia
  • Biljana Stojimirović Clinic of Nephrology, University of Belgrade, School of Medicine
DOI: https://doi.org/10.2298/1314
Ključne reči: peritoneal dialysis||, ||dijaliza, peritoneumska, rabbits||, ||zečevi, diseases models, animal||, ||bolest, modeli na životinjama, peritoneum||, ||peritoneum, microscopy, electron||, ||mikroskopija, elektronska, histology||, ||histologija,

Sažetak


Uvod/Cilj. Poslednjih godina u porastu je broj bolesnika sa terminalnom bubrežnom insuficijencijom koji se leče dijalizom. Dugotrajna primena peritoneumske dijalize praćena je razvojem strukturnih i funkcionalnih promena trbušne maramice. Cilj rada bio je analiza ultrastrukturnih promena mezotelnog prekrivača i submezotelnog vezivnog tkiva trbušne maramice na modifikovanom neuremijskom infuzionom modelu peritoneumske dijalize kod zeca. Metode. Istraživanje je izvedeno na pet zdravih zečeva rase Činčila. Hirurške procedure implantacije i vađenja peritoneumskog katetera, prevencija opstrukcije katetera, zaštita životinja od infekcije, instilacije dijalizata, uzimanje i priprema uzoraka peritoneuma za histološku analizu vršeni su prema prethodno opisanim protokolima. Rezultati. Eksperimentalne životinje dobro su podnosile aklimatizaciju, hirurške procedure i instilaciju dijalizata, nije bilo komplikacija tokom perioda praćenja. Analizom uzoraka tkiva životinja kojima je rađena peritoneumska dijaliza evidentirane su promene u mezotelnom sloju i u vezivnom tkivu submezotelnog sloja korišćenjem transmisione elektronske mikroskopije (TAM). Mezotelne ćelije koje su u direktnom kontaktu sa dijaliznom tečnosti podležu deskvamaciji, niske su, ljuspaste, sa proširenjem u predelu perinukleusnog dela; imaju brojne mikrovile u apikalnom delu i mnogobrojne transportne vezikule. Ispod mezotelnih ćelija, uočavaju se prošireni limfni sudovi i snopovi kolagenih vlakana koji se pružaju u različitim pravcima i okružuju tela i razgranate produžetke fibroblasta. Nukleusi fibroblasta su pretežno euhromatski, a u citoplazmi se uočavaju brojne sekretorne granule, vezikule, kao i mitohondrije. Zaključak. Modifikovani model peritoneumske dijalize kod zečeva koji smo koristili u ovoj studiji je praktičan, reproducibilan i ne zahteva sofisticiranu tehnologiju, te je pogodan za dalja histološka istraživanja dugotrajnih efekata dijaliznih rastvora na membranu peritoneuma zečeva.

 

 


Biografije autora

Snežana Žunić-Božinovski, Institute of Pathophysiology, Faculty of Medicine, University of Belgrade, Serbia
Institute of pathophysiology
Dušan Trpinac, Institute of Histology and Embriology, University of Belgrade, School of Medicine
Institute of Histology and Embriology
Željko Laušević, Institute for Digestive Diseases,University of Belgrade, School of Medicine
Institute for Digestive Diseases
Slobodan Krstić, Institute for Digestive Diseases,University of Belgrade, School of Medicine
Institute for Digestive Diseases
Dejan Oprić, Institute of Pathology, University of Belgrade, School of Medicine
Institute of Pathology
Jasna Trbojević-Stanković, Department of Hemodialysis and Nephrology, Clinical Hospital Center „Dr Dragisa Misovic“, Belgrade, Serbia
Department of Hemodialysis and Nephrology

Reference

Brown EA, Johansson L Epidemiology and management of end-stage renal disease in the elderly. Nat Rev Nephrol 2011; 7(10): 591−8.

De Nicola L, Donfrancesco C, Minutolo R, Lo Noce C, De Curtis A, Palmieri L, et al. Epidemiology of chronic kidney disease in Italy: current situation and contribution of the CARHES study. G Ital Nefrol 2011; 28(4): 401−7. (Italian)

Jovanović N, Nešić V, Laušević M, Stojimirović B. peritoneal dialysis adequacy. Srp Аrh Celok Lek 2005; 133(11−12): 498−504. (Serbian)

Lamiere N, Van Biesen W. What can we learn from registry data on peritoneal dialysis outcome? Ronco C, Crepaldi C, Cruz DN, editors. Peritoneal Dialysis – from basic concepts to clinical excellence. Basel: Karger; 2009. p. 227−36.

Stojimirović BB, Obradović MM, Trpinac DP, Milutinović DD, Obradović DI, Nešić VB. Mesothelial paracrystalline inclusions in continuous ambulatory peritoneal dialysis patients. Perit Dial Int 2001; 21(Suppl 3): S54−7.

Witowski J, Jorres A. Peritoneal dialysis: a biological membrane with nonbiological fluid. Ronco C, Crepaldi C, Cruz DN, editors. Peritoneal Dialysis – from basic concepts to clinical excellence. Basel: Karger; 2009. p. 27−34.

William JD, Craig KJ, Topley N, Von Ruhland C, Fallon M, New-man GR, et al. Morphological changes in the peritoneal mem-brane of patients with renal disease. J Am Soc Nephrol 2002; 13(2): 470−9.

Stojimirović B, Obradović M, Trpinac D, Milutinović D, Nešić V. Peritoneal lining cells changes in end stage kidney disease. Facta Universitatis 2000; 7(1): 97−101.

Trpinac DP, Stojimirović BB, Obradović MM, Milutinović DD, Obrasdović DI, Nešić VB. Effect of uremia and peritoneal dialy-sis on peritoneal mesothelial cells. Vojnosanit Pregl 2002; 59(1): 17−21. (Serbian)

Stojimirović BB, Obradović MM, Trpinac DP, Milutinović DD, Obradović DI, Nešić B. Characteristics of lamellar bodies in peritoneum Facta Universitatis 2002; 9(2): 171−4.

Trbojević-Stanković J, Obradović M, Cemerikić-Martinović V, Trpinac D, Lausević Z, Stojimirović B. Immunohistochemical study of pathological alterations of peritoneum in patients with terminal renal insufficiency and on peritoneal dialysis. Vojnosanit Pregl 2011; 68(7): 556−60.

Smit W, Parikova A, Struijk DG, Krediet RT. The difference in causes of early and late ultrafiltration failure in peritoneal dialysis. Perit Dial Int 2005; 25(Suppl 3): S41−5.

Stojimirović B, Jovanović N, Laušević Ž, Krstić S, Obradović M, Žunić-Božinovski S. First histological findings in rabbit model of peritoneal dialysis. Acta Veterinaria. 2010; 60 (5−6): 625−32.

Stojimirovic B, Jovanovic N, Lausevic Z, Krstic S, Trbojevic-Stankovic J, Trpinac D, et al. Possibilities of histological studies in non-uremic rabbit model of peritoneal dialysis. J AnimVet Adv 2011; 10(11): 1414−20.

Žunić-Božinovski S., Laušević Ž, Krstić S, Jovanović N, Trbojević-Stanković J, Stojimirović B. An experimental, non-uremic rabbit model of peritoneal dialysis. Physiol Res 2008; 57(2): 253−60.

Hirihara I, Kusano E, Yanagiba S, Miyata Y, Ando Y, Muto S, et al. Peritoneal injury by methylglyoxal in peritoneal dialysis. Perit Dial Int 2006; 26(3): 380−92.

De Vriese AS, Mortier S, Lamiere NH. What happens to the peritoneal membrane in long term peritoneal dialysis? Perit Dial Int 2001; 21(Suppl 3): S9−18.

William JD, Craig KJ, Topley N, Von Ruhland C, Fallon M, New-man GR, et al. Morphological changes in the peritoneal mem-brane of patients with renal disease. J Am Soc Nephrol 2002; 13(2): 470−9.

Peng YM, Shu ZJ, Xiao L, Sun L, Tang WB, Huang Y, et al. A new non-uremic rat model of long term peritoneal dialysis. Physiol Res 2011; 60(1): 157−64.

Santamaria B, Ucero AC, Reyero A, Selgas R, Ruiz-Ortera M, Catalan M, et al. 3,4-Dideoxyglucosone-3-one as a mediator of peritoneal demesothelization. Nephrol Dial Transplant 2008; 23(10): 3307−15.

Yamamoto T, Tomo T, Okabe E, Namoto S, Suzuki K, Hirao Y. Gluthatione depletion as a mechanism of 3,4-Dideoxyglucosone-3-one-induced cytotoxicity in human peri-toneal mesothelial cells: role in bioimcompatibility of perito-neal dialysis fluid. Nephrol Dial Transplant 2009; 24(5): 1436–42.

Aroeira LS, Aguilera A, Sanchez-Tomero JA, Bajo MA, del Peso G, Jimenez-Heffernan JA, et al. Epithelial to mesenchimal transition and peritoneal membrane failure in peritoneal dialysis patients: pathologic significance and potential therapeutic options. J Am Soc Nephrol 2007; 18(7): 2004−13.

Witowksi J, Ksiazek K, Jorres A. Glucose-induced mesothelial cells senescence and peritoneal neoangiogenesis and fibrosis. Perit Dial Int 2008; 28(Suppl 5): S34–7.

Objavljeno
2017/01/20
Broj časopisa
Vol. 70 Br. 11 (2013)
Rubrika
Originalni članak