A Comprehensive Review of the Direct Peptide Reactivity Assay in Skin Sensitization Testing: Applicability, Limitations, and Considerations

Elma Omeragić; Belma Imamović; Mirza Dedić; Indira Mujezin; Ervina Bečić

doi:10.5937/arhfarm74-49552

Elma Omeragić University of Sarajevo – Faculty of Pharmacy
Belma Imamović University of Sarajevo – Faculty of Pharmacy
Mirza Dedić University of Sarajevo – Faculty of Pharmacy
Indira Mujezin University of Sarajevo – Faculty of Health Studies; Public Institution Health Centre of Sarajevo Canton
Ervina Bečić University of Sarajevo – Faculty of Pharmacy

DOI: https://doi.org/10.5937/arhfarm74-49552

Keywords: DPRA, in chemico method, skin sensitization

Abstract

Skin sensitization is a crucial endpoint in the safety assessment of chemicals, with the Direct Peptide Reactivity Assay (DPRA) emerging as a valuable in chemico method for evaluating a substance's sensitization potential. This review delves into the principles, applicability, and limitations of the DPRA within the context of the Adverse Outcome Pathway (AOP) framework for skin sensitization. We examine the DPRA's role in addressing the molecular initiating event of skin sensitization, its integration into Integrated Approaches to Testing and Assessment (IATA), and its performance in predicting sensitizers. The review also highlights the challenges in testing certain categories of chemicals and the importance of considering the DPRA's results alongside other complementary methods. By providing a comprehensive overview of the DPRA, this review aims to inform researchers, regulators, and clinicians about its utility and limitations in the context of skin sensitization testing.

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