APPLICABILITY OF LDL-CHOLESTEROL CALCULATION FORMULAS IN HYPERTRIGLYCERIDEMIA: INSIGHTS FROM THE VOJVODINA

LDL-C FORMULAS IN HYPERTRIGLYCERIDEMIA

  • Dragana Žuvić Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0000-0002-0114-2989
  • Stanislava Nikolić Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0000-0002-7859-0923
  • Romana Mijović Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0000-0002-4034-1585
  • Branislava Ilinčić Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0000-0001-6160-8173
  • Aneta Ranđelović Živković Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0009-0008-9714-4831
  • Dušan Sedlarević Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0009-0008-2075-4754
  • Velibor Čabarkapa Faculty of Medicine, University of Novi Sad; Center for Laboratory Diagnostics, University Clinical Center of Vojvodina, Novi Sad, Serbia https://orcid.org/0000-0001-7166-3379
DOI: https://doi.org/10.5937/jomb0-61948
Keywords: lipids; cholesterol, LDL; hypertriglyceridemia; Sampson formula

Abstract


Background: LDL-cholesterol is a key parameter for assessing the risk of atherosclerotic cardiovascular disease. Direct measurement of LDL-cholesterol is not always possible due to practical or financial reasons, making the use of calculation formulas essential for its evaluation. The aim of this study was to examine the applicability of four different formulas for calculating LDL-cholesterol compared to direct method in patients with serum triglyceride levels from 4.5 to 9.0 mmol/L in the population of Vojvodina.

Methods: The retrospective study included 272 subjects whose lipid status parameters were measured using standard laboratory methods between June 2022 and June 2023. The participants had serum triglyceride levels ranging from 4.5 and 9.0 mmol/L. LDL-cholesterol was determined by direct method (d-LDL-C) on Alinity c analyser (Abbott). Additionally, LDL-cholesterol levels were calculated for this population using the formulas proposed by Friedewald, Sampson, Anandaraja and Martin.

Results: In the studied population, the average age was 52 years and the median concentration of triglycerides was 5.48 (4.94-6.58) mmol/L. A statistically significant positive correlation was found between d-LDL-C and all calculated parameters (P<0.001). The lowest mean difference was observed between d-LDL-C and the Sampson formula (MD=-0.032). When comparing d-LDL-C and LDL-cholesterol values obtained by calculation, only the formula of Sampson et al. did not show a statistically significant difference (P=0.240).

Conclusion: In the studied population of patients with hypertriglyceridemia, the formula by Sampson et al. showed the best performance in comparison to the others tested and its application could be considered for the Vojvodina population.

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Published
2026/02/04
Issue
Ahead of print
Section
Original paper

Copyright (c) 2026 Dragana Žuvić, Stanislava Nikolić, Romana Mijović, Branislava Ilinčić, Aneta Ranđelović Živković, Dušan Sedlarević, Velibor Čabarkapa

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