.

Evaluation of a new von Willebrand Factor multimers assay kit

  • Marika Pikta North Estonia Medical Centre https://orcid.org/0000-0002-5891-7589
  • Timea Szanto Coagulation Disorders Unit, Department of Hematology, Helsinki University Hospital Comprehensive Cancer Centre and University of Helsinki, Helsinki, Finland
  • Margus Viigimaa Department of Health Technologies, Tallinn University of Technology, Tallinn, Estonia; Centre of Cardiology, North Estonia Medical Centre, Tallinn, Estonia
  • Sandra Lejniece Riga Stradins University, Riga, Latvia; Chemotherapy and Hematology Clinic, Riga East University Hospital, Riga, Latvia
  • Dārta Balode Riga Stradins University, Riga, Latvia; Chemotherapy and Hematology Clinic, Riga East University Hospital, Riga, Latvia
  • Kadri Saks Hematology Department, Tallinn Children`s Hospital, Tallinn, Estonia
  • Valdas Banys Vilnius University, Faculty of Medicine, Institute of Biomedical Sciences, Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Vilnius, Lithuania

Sažetak


Accurate diagnosis and classification of von Willebrand disease are essential for optimal management.  The von Willebrand factor multimers analysis is in the phenotypic classification, especially in discrepant cases, an integral part of the diagnostic process. The aim of this study was to evaluate the performance of a new Hydragel 11VWF multimer assay (H11VW). Results: Comparison study did not reveal any significant difference between H5VW and H11VW.  The assessment of within-subject results, using H5VW and H11VW, demonstrates the reproducibility of the method in both healthy and VWD patients’ samples collected over time, with identical multimeric pattern on densitometric curves.Conclusion: This assay demonstrated acceptable performance, produced within-day results and can be used in routine practice for the visual investigation of gel and quantitative estimation of VWF multimer fractions.

Reference

1. Favaloro EJ. Von Willebrand disease: local diagnosis and management of a globally distributed bleeding disorder. Semin Thromb Hemost 2011; 37(5): 440-55.
2. Szanto T, Lassila R, Funding E, et al. Von Willebrand disease—the Nordic perspective. Ann Blood 2018; 3: 2.
3. Nummi V, Lassila R, Joutsi-Korhonen L, et al. Comprehensive re-evaluation ofhistorical von Willebrand disease diagnosis in association with whole blood platelet aggregation and function. Int J Lab Hem 2018; 40: 304-311.
4. Roberts JC, Flood VH. Laboratory diagnosis of von Willebrand disease. Int J Lab Hematol 2015; 37(Suppl): 11‐17.
5. Torres R, Genzen JR, Levene MJ. Clinical Measurement of von Willebrand Factor by Fluorescence Correlation Spectroscopy. Clin Chem 2012; 58: 1010-1018
6. Meijer P, Haverkate F. An external quality assessment program for von Willebrand factor laboratory analysis: an overview from the European concerted action on thrombosis and disabilities foundation. Semin Thromb Hemost 2006; 32: 485-491.
7. Ledford-Kraemer MR. Analysis of von Willebrand factor structure by multimer analysis. Am J Hematol 2010; 85: 510-514.
8. Oliver S, Lau KKE, Chapman K, Favaloro EJ. Laboratory testing for von Willebrand factor multimers. Methods Mol Biol 2017; 1646: 495–511
9. Crist RA, Heikal NM, Rodgers GM, Grenache DG, Smock KJ. Evaluation of a new commercial method for von Willebrand factor multimeric analysis. Int J Lab Hem 2018; 40: 586–591.
10. Favaloro EJ, Oliver S. Evaluation of a new commercial von Willebrand factor multimer assay. Haemophilia. 2017; 23: e373-e377.
11. Pikta M, Zemtsovskaja G, Bautista H, et al. Preclinical evaluation of a semi-automated and rapid commercial electrophoresis assay for von Willebrand factor multimers. J Clin Lab Anal 2018; 32: e22416.
12. Bowyer AE, Goodfellow KJ, Seidel H, et al. Evaluation of a semi-automated von Willebrand factor multimer assay, the Hydragel 5 von Willebrand multimer, by two European Centers. Res Pract Thromb Haemost 2018; 2: 790–799.
13. Pikta M, Zolotareva V, Tõnne J, Viigimaa M, Banys V. Implementation and Verification of New VWF: Ac Assay System with Components from Different Manufacturers. Laboratorine medicina 2016; 72: 185-188
14. Favaloro EJ. Rethinking internal quality control and external quality assessment for laboratory diagnostics of von Willebrand disease. Ann Blood 2019; 4: 4.
Objavljeno
2020/08/18
Rubrika
Original paper