Асоцијације експресије Галектина-3 и ЛГАЛС-3 (рс4652) Варијација гена са ризиком болести коренске артерије код дијабетичара

  • Samy Hassan 1Medical Biochemistry Department, Faculty of Medicine, University of Zagazig, Zagazig, Egypt
  • Mohamed Hassaan
  • Basma Demasy
  • Norhan Sabbah assistant professor of medical biochemistry ,Zagazig university
DOI: https://doi.org/10.5937/jomb0-30424
Ključne reči: ЛГАЛС-3 (рс4652), израз Галецтин-3, ИЛ-6, Т2ДМ, ЦАД

Sažetak


Позадински ген Галектин-3 који је кодиран геном лектин галактозида који веже лектин-3 (ЛГАЛС-3) служи као важан генетски фактор у дијабетес мелитусу типа 2 (Т2ДМ) и његовим кардиоваскуларним препрекама у различитим популацијама. Желели смо да изазовемо про-упални ефекат галектина-3 како је утврђено интерлеукином-6 (ИЛ-6) нивоа у серуму и за истраживање односа између галектина-3 (ЛГАЛС-3 рс4652) варијација гена и ниво његове експресије са коронарном артеријском болешћу (ЦАД) ризик код Т2ДМ египатских пацијената.Методе: 112 витких испитаника је упоређено са 100 Т2ДМ без ЦАД-а и 84 Т2ДМ са ЦАД-ом. Ланчана реакција полимеразе тетра-примера за појачавање, коришћена је за тестирање ЛГАЛС-3 (рс4652) и израз галектина-3 тестиран је квантитативном ланчаном реакцијом полимеразе у стварном времену. Серум ИЛ-6 је мерен помоћу имуносорбентног теста везаног ензимом. Резултати: Открили смо да је преваленца ЛГАЛС-3 (рс4652) Нивои генотипа генотипа и експресије гена у Т2ДМ са ЦАД-ом били су значајно виши од додатне две групе и такође су позитивно повезани са нивоима циркулације ИЛ-6, носачи алела Ц (АЦ + ЦЦ) имали су значајно веће релативне нивое експресије Галектин-3 у поређењу са носиоцима алела А (АА).Закључак: Закључили смо да су нивои експресије галактина-3 и ЛГАЛС-3 (рс4652) АЦП генотип је био фактор ризика од коронарне артерије код дијабетичара типа два међу египатским узорком.

Reference

1. Alturfan AA, Basar I, Emekli-Alturfan E, Ayan F, Koldas L, and Emekli N. Galectin-3 and plasma cytokines in patients with acute myocardial infarction. Lab Med 2014;45:336-341.
2. American Diabetes Association. 15. Diabetes Care in the Hospital: Standards of Medical Care in Diabetes-2019. Diabetes Care 2019;42(Suppl 1): S173-S181.
3. Atabaki M, Hashemi M, Daneshvar H, and Alijani E. Lectin, galactoside-binding, soluble, 3 rs4652 A/C gene variation and the risk for rheumatoid arthritis. Biomed Rep 2017;6:251-255.
4. Bobronnikova L. Galectin-3 as a potential biomarker of metabolic disorders and cardiovascular remodeling in patients with hypertension and type 2 diabetes. Vessel Plus 2017;1:61-67.
5. Buccolo G, and David H. Quantitative determination of serum triglycerides by use of enzymes. Clin Chem 1973;19:476-482.
6. De Boer RA, Verweij N, van Veldhuisen DJ, Westra HJ, Bakker SJ, Gansevoort RT, et al. A genome-wide association study of circulating galectin-3. PLoS One 2012;7:e47385.
7. Debrunner M, Schuiki E, Minder E, Straumann E, Naegeli B, Mury R, et al. Proinflammatory cytokines in acute myocardial infarction with and without cardiogenic shock. Clin Res Cardiol 2008;97:298-305.
8. Djordjevic A, Dekleva M, Zivkovic M, Stankovic A, Markovic Nikolic N, Alavantic D, et al. Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. Mol Biol Rep 2018;45:2227-2236.
9. Djordjevic A, Zivkovic M, Stankovic A, Zivotic I, Koncar I, Davidovic L, et al. Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. J Clin Lab Anal 2016;30:1150-1157.
10. Dong H, Wang ZH, Zhang N, Liu SD, Zhao JJ, and Liu SY. Serum Galectin-3 level, not Galectin-1, is associated with the clinical feature and outcome in patients with acute ischemic stroke. Oncotarget 2017;8:109752-109761.
11. Fashanu OE, Heckbert SR, Aguilar D, Jensen PN, Ballantyne CM, Basu S, et al. Galectin-3 and Venous Thromboembolism Incidence: the Atherosclerosis Risk in Communities (ARIC) Study. Res Pract Thromb Haemost 2017;1:223-230.
12. Filer A, Bik M, Parsonage GN, Fitton J, Trebilcock E, Howlett K, et al. Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways. Arthritis Rheum 2009;60:1604-1614.
13. Frieldewald WT, Levy RI, and Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499-502.
14. Glovaci D, Fan W, and Wong ND. Epidemiology of Diabetes Mellitus and Cardiovascular Disease. Curr Cardiol Rep 2019;21:21.
15. Hegazi R, El-Gamal M, Abdel-Hady N, and Hamdy O. Epidemiology of and Risk Factors for Type 2 Diabetes in Egypt. Ann Glob Health 2015;81:814-820.
16. Hodeib H, Hagras MM, Abdelhai D, Watany MM, Selim A, Tawfik MA, et al. Galectin-3 as a prognostic biomarker for diabetic nephropathy. Diabetes Metab Syndr Obes 2019;12:325-331.
17. Humphry RW, Cameron A, and Gunn GJ. A practical approach to calculate sample size for herd prevalence surveys. Prev Vet Med 2004;65:173-188.
18. Iacobini C, Amadio L, Oddi G, Ricci C, Barsotti P, Missori S, et al. Role of galectin-3 in diabetic nephropathy. J Am Soc Nephrol 2003;14(8 Suppl 3): S264-S270.
19. Iacobini C, Menini S, Ricci C, Scipioni A, Sansoni V, Cordone S, et al. Accelerated lipid-induced atherogenesis in galectin-3-deficient mice: role of lipoxidation via receptor-mediated mechanisms. Arterioscler Thromb Vasc Biol 2009;29:831-836.
20. Kaplan A, and Glucose K. Clin Chem. The CV Mosby Co., St Louis, Toronto, Princeton, 1984, 436 p.
21. Koshy L, Anju AL, Harikrishnan S, Kutty VR, Jissa VT, Kurikesu I, et al. Evaluating genomic DNA extraction methods from human whole blood using endpoint and real-time PCR assays. Mol Biol Rep 2017;44:97-108.
22. Lind L. Circulating markers of inflammation and atherosclerosis. Atherosclerosis 2003;169:203-214.
23. Livak KJ, and Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method. Methods 2001;25:402-408.
24. MacKinnon AC, Liu X, Hadoke PW, Miller MR, Newby DE, and Sethi T. Inhibition of galectin-3 reduces atherosclerosis in apolipoprotein E-deficient mice. Glycobiology 2013;23:654-663.
25. Madrigal-Matute J, Lindholt JS, Fernandez-Garcia CE, Benito-Martin A, Burillo E, Zalba G, et al. Galectin-3, a biomarker linking oxidative stress and inflammation with the clinical outcomes of patients with atherothrombosis. J Am Heart Assoc 2014;3:e000785.
26. Maiolino G, Rossitto G, Pedon L, Cesari M, Frigo AC, Azzolini M, et al. Galectin-3 predicts long-term cardiovascular death in high-risk patients with coronary artery disease. Arterioscler Thromb Vasc Biol 2015;35:725-732.
27. Mancia G, Fagard R, Narkiewicz K, Redón J, Zanchetti A, Böhm M, et al. 2013 ESH/ESC Guidelines for the management of arterial hypertension: the Task Force for the management of arterial hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC). J Hypertens 2013;31:1281-1357.
28. Menini S, Iacobini C, Blasetti Fantauzzi C, Pesce CM, and Pugliese G. Role of Galectin-3 in Obesity and Impaired Glucose Homeostasis. Oxid Med Cell Longev 2016;2016:9618092.
29. Naito HK. Cholesterol. Clin Chem. The C.V. Mosby Co. St Louis, Toronto, Princeton, 1984, 1194-11206 and 437.
30. Naito HK. High-density lipoprotein (HDL) cholesterol. In: Kaplan A, et al. (eds) Clin Chem. The C.V. Mosby Co. St Louis, Toronto, Princeton, 1984, 1207-1213 and 437.
31. Ozturk D, Celik O, Satilmis S, Aslan S, Erturk M, Cakmak HA, et al. Association between serum galectin-3 levels and coronary atherosclerosis and plaque burden/structure in patients with type 2 diabetes mellitus. Coron Artery Dis 2015;26:396-401.
32. Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, et al. Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction. Arterioscler Thromb Vasc Biol 2008;28:433-440.
33. Pugliese G, Iacobini C, Ricci C, Blasetti Fantauzzi C, and Menini S. Galectin-3 in diabetic patients. Clin Chem Lab Med 2014;52:1413-1423.
34. Qu D, Liu J, Lau CW, and Huang Y. IL-6 in diabetes and cardiovascular complications. Br J Pharmacol 2014;171:3595-3603.
35. Reiner Z. New ESC/EAS Guidelines for the management of dyslipidaemias any controversies behind the consensus? Eur J Cardiovasc Prev Rehabil 2011;18:724-727.
36. Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract 2019;157:107843.
37. Sciacchitano S, Lavra L, Morgante A, Ulivieri A, Magi F, De Francesco GP, et al. Galectin-3: One Molecule for an Alphabet of Diseases, from A to Z. Int J Mol Sci 2018;19:379.
38. Steffes M, Cleary P, Goldstein D, Little R, Wiedmeyer HM, Rohlfing C, et al. Hemoglobin A1c measurements over nearly two decades: sustaining comparable values throughout the diabetes control and complications trial and the epidemiology of diabetes interventions and complications study. Clin Chem 2005;51:753-758.
39. Tan KCB, Cheung CL, Lee ACH, Lam JKY, Wong Y, and Shiu SWM. Galectin-3 and risk of cardiovascular events and all-cause mortality in type 2 diabetes. Diabetes Metab Res Rev 2019;35:e3093.
40. Tian L, Chen K, Cao J, Han Z, Gao L, Wang Y, et al. Galectin-3-induced oxidized low-density lipoprotein promotes the phenotypic transformation of vascular smooth muscle cells. Mol Med Rep 2015;12:4995-5002.
41. Tuñón J, Blanco-Colio L, Cristóbal C, Tarín N, Higueras J, Huelmos A, et al. Usefulness of a combination of monocyte chemoattractant protein-1, galectin-3, and N-terminal probrain natriuretic peptide to predict cardiovascular events in patients with coronary artery disease. Am J Cardiol 2014;113:434-440.
42. Van Snick J. Interleukin-6: an overview. Annu Rev Immunol 1990;8:253-278.
43. Zhang Y, Wang Y, Zhai M, Gan T, Zhao X, Zhang R, et al. Influence of LGALS3 gene polymorphisms on susceptibility and prognosis of dilated cardiomyopathy in a Northern Han Chinese population. Gene 2018;642:293-298.
44. Zhu W, Sano H, Nagai R, Fukuhara K, Miyazaki A, and Horiuchi S. The role of galectin-3 in endocytosis of advanced glycation end products and modified low density lipoproteins. Biochem Biophys Res Commun 2001;280:1183-1188.
Objavljeno
2021/07/08
Broj časopisa
Vol. 40 Br. 4 (2021): JMB
Rubrika
Original paper

Sva prava zadržana (c) 2021 Samy Hassan, Mohamed Hassaan, Basma Demasy, Norhan Sabbah

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