Uticaj različitih uslova skladištenja uzoraka i njihove mikrobiološke kontaminacije na koncentracije rutinskih biohemijskih parametara

  • Tamara Gojković Katedra za medicinsku biohemiju, Univerzitet u Beogradu - Farmaceutski fakultet
  • Sandra Vladimirov Katedra za medicinsku biohemiju, Univerzitet u Beogradu - Farmaceutski fakultet
  • Tamara Antonić Katedra za medicinsku biohemiju, Univerzitet u Beogradu - Farmaceutski fakultet
  • Nataša Bogavac-Stanojević Katedra za medicinsku biohemiju, Univerzitet u Beogradu - Farmaceutski fakultet
  • Katarina Novović Institut za molekularnu genetiku i genetičko inženjerstvo, Univerzitet u Beogradu
  • Vesna Spasojević-Kalimanovska Katedra za medicinsku biohemiju, Univerzitet u Beogradu - Farmaceutski fakultet
  • Brankica Filipić Katedra za mikrobiologiju i imunologiju, Univerzitet u Beogradu - Farmaceutski fakultet
DOI: https://doi.org/10.5937/jomb0-40360
Ključne reči: ciklusi zamrzavanja-odmrzavanja, mikrobiološka kontaminacija, konzervans kalijum-fluorid, preanalitička faza

Sažetak


Uvod: Preanalitička (PA) faza je složen proces koji čine: prikupljanje, rukovanje, transport i skladištenje uzoraka, te predstavlja najznačajniji izvor laboratorijskih grešaka. Cilj ovog istraživanja bio je da se ispita kako različiti antikoagulansi, uslovi skladištenja i ciklusi zamrzavanja-odmrzavanja (FTC) utiču na koncentracije osnovnih biohemijskih parametara. Pored toga, ispitivano je prisustvo i efekat mikrobiološke kontaminacije uzoraka tokom rutinskog laboratorijskog rada.

Metode: Dva pool-a plazme (EDTA i natrijum-fluorid/kalijum oksalat (NaF)) su skladištena na +4C˚/-20˚C. Koncentracije ukupnog holesterola (TC), glukoze, triglicerida (TG), uree, ukupnih proteina (TP) i albumina određene su rutinskim metodama na Ilab 300+ analizatoru. Mikrobiološka kontaminacija uzoraka je potvrđena 16S rRNA sekvencioniranjem. Eksperiment je sproveden tokom 5 uzastopnih dana: 1.dan – svež uzorak, 2.dan–1.FTC, 3.dan–2.FTC, 4.dan–3.FTC, 5.dan–4.FTC. Završnu tačku eksperimenta predstavljala je pojava bakterija u dva uzastopna uzorka.

Rezultati: Tokom 4 FTC koncentracije uree u plazmi se nisu značajno razlikovale. Koncentracija glukoze je bila stabilna u EDTA+4˚C i NaF-20˚C do 3.FTC (P=0.008, P=0.042, redom). Promene u koncentraciji proteina ispoljile su cik-cak obrazac. Koncentracije TG su se značajno promenile u uzorku EDTA-20˚C nakon 1. i 4. FTC-a (P=0.022, P=0.010, redom). U uzorcima NaF plazme nije došlo do bakterijske kontaminacije tokom 4 FTC.

 

Zaključci: Koncentracije uree i glukoze su bile stabilne tokom trajanja eksperimenta. Promene u koncentracijama lipida i proteina nakon FTC prate složene obrasce. Rast bakterija nije primećen u uzorcima NaF plazme, te upotreba ovog antikoagulansa može biti opravdana u analitičkim procedurama podložnim uticaju mikrobiološke kontaminacije.

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Objavljeno
2023/12/25
Broj časopisa
Vol. 43 Br. 3 (2024): Journal of Medical Biochemistry
Rubrika
Original paper

Sva prava zadržana (c) 2023 Tamara Gojkovic, Sandra Vladimirov, Tamara Antonic, Natasa Bogavac-Stanojevic, Katarina Novovic, Vesna Spasojevic-Kalimanovska, Brankica Filipic

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