Asocijacija varijanti u genima hsa-miR-27a i hsa-miR-146a sa muškim sterilitetom

  • Zorana Dobrijević University of Belgrade - Institute for the Application of Nuclear Energy
  • Srećko Rajovski
  • Suzana Matijašević Joković
  • Nikoleta Milanović
  • Nemanja Radovanović
  • Miloš Brkušanin
  • Dušanka Savić-Pavićević
  • Goran Brajušković
DOI: https://doi.org/10.5937/jomb0-50876
Ključne reči: muški sterilitet, rs2910164, rs895819, polimorfizam, miR-27a, miR-146a

Sažetak


Uvod: miRNA molekule odlikuje izuzetan potencijal za upotrebu u vidu dijagnostičkih i prognostičkih biomarkera i terapijskih targeta kod muškog steriliteta i bolesti reproduktivnog sistema. Cilj ove studije  bilo je ispitivanje asocijacije dveju funkcionalnih genetičkih varijanti gena  hsa-miR-27a (rs2910164) i hsa-miR-146a (rs895819) i idiopatskog muškog steriliteta  u populaciji Severne Makedonije i testiranje njihove asocijacije  sa vrednostima seminalnih parametara.

Metode: Grupu ispitanika činilo je 158 muškaraca sa inicijalnom dijagnozom idiopatskog muškog steriliteta. Kontrolna grupa uključivala je 126 muškaraca volontera podudarne starosti koji su očevi barem jednog deteta.

Rezultati: Po prvi put pokazana je asocijacija manje učestalog alela C varijante rs2910164 sa povišenim rizikom za za pojavu steriliteta kod muškaraca sa astenoteratozoospermijom. Takođe, rezultati koji ukazuju na asocijaciju alela C sa nižom stopom vijabilnosti spermatozoida predstavljaju novi nalaz. Nije pokazana asocijacija varijante rs895819 sa rizikom za pojavu muškog steriliteta. Ipak, procenat učesnika u studiji sa genotipom CC među muškarcima sa dijagnozom astenoteratozoospermije bio je 0, dok je u grupi kontrola iznosio 7.2%. Pored toga, ustanovljen je efekat varijante rs895819 na brzu progresivnu pokretljivost spermatozoida.

Zaključci: Asocijacija rs2910164 i rs895819 sa idiopatskim muškim sterilitetom uopšteno nije potvrđena. S druge strane, za obe navedene varijante pokazana je asocijacija sa rizikom za pojavu određenih tipova muškog steriliteta,  kao i sa abnormalnostima spermatozoida. Navedene rezultate potrebno je potvrditi  u budućim studijama u drugim etničkim grupama.

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Objavljeno
2024/07/19
Broj časopisa
Vol. 43 Br. 6 (2024): Journal of Medical Biochemistry
Rubrika
Original paper

Sva prava zadržana (c) 2024 Zorana Dobrijević, Srećko Rajovski, Suzana Matijašević Joković, Nikoleta Milanović, Nemanja Radovanović, Miloš Brkušanin, Dušanka Savić-Pavićević, Goran Brajušković

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