POTENCIJAL ANTAGONISTA Bacillus spp. ZA SUZBIJANJE FITOPATOGENA Xanthomonas euvesicatoria

  • Ivana Pajčin Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, 21000 Novi Sad, Bulevar cara Lazara 1, Srbija
  • Vanja Vlajkov Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, 21000 Novi Sad, Bulevar cara Lazara 1, Srbija
  • Zorana Rončević Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, 21000 Novi Sad, Bulevar cara Lazara 1, Srbija
  • Jelena Dodić Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, 21000 Novi Sad, Bulevar cara Lazara 1, Srbija
  • Mila Grahovac Univerzitet u Novom Sadu, Poljoprivredni fakultet, 21000 Novi Sad, Trg Dositeja Obradovića 8, Srbija
  • Maja Ignjatov Institut za ratarstvo i povrtarstvo, 21000 Novi Sad, Maksima Gorkog 30, Srbija
  • Jovana Grahovac Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, 21000 Novi Sad, Bulevar cara Lazara 1, Srbija
DOI: https://doi.org/10.5937/jpea24-27076
Ključne reči: antimikrobna aktivnost, paprika, bakteriozna pegavost, biomasa, antimikrobna jedinjenja, osetljivost

Sažetak


Bakterijski patogeni Xanthomonas euvesicatoria predstavljaju glavne uzročnike bakteriozne pegavosti, stoga predstavljaju značajan problem u poljoprivrednoj praksi usled izazivanja velikih gubitaka prinosa u proizvodnji paprike i paradajza. Uobičajena sredstva za suzbijanje i kontrolu ovih fitopatogena predstavljaju preparati na bazi bakra. Međutim, razvoj rezistentnosti prema pesticidima na bazi bakra doveo je do zaokreta u polju suzbijanja ovih patogena ka primeni biopesticida, odnosno bioloških kontrolnih agenasa. Bakterije roda Bacillus i njihovi metaboliti sa izraženom antimikrobnom aktivnošću protiv ciljanih fitopatogena predstavljaju najperspektivnije aktivne komponente biokontrolnih preparata za zaštitu bilja. U ovom istraživanju nekoliko sojeva roda Bacillus ispitano je sa ciljem odabira antagonista dovoljno efikasnih u suzbijanju sojeva Xanthomonas euvesicatoria, koji su izolovani sa listova paprike sa simptomima bakteriozne pegavosti. Prilikom testiranja antimikrobne aktivnosti uzoraka kultivacionih tečnosti, koji sadrže i biomasu testiranih antagonista, najbolji rezultati u suzbijanju testiranih fitopatogena su postignuti primenom izolata Bacillus sp3. Sa druge strane, prilikom testiranja antimikrobne aktivnosti supernatanata oslobođenih biomase antagonista, koji sadrže samo produkovana antimikrobna jedinjenja, izolati Bacillus sp1 i Bacillus sp2 su pokazali najveću antimikrobnu aktivnost protiv fitopatogena Xanthomonas euvesicatoria. Rezultati ovog istraživanja predstavljaju osnovu za dalji razvoj bioprocesnih rešenja za proizvodnju biopesticida na bazi biomase ili antimikrobnih jedinjenja koja proizvode antagonisti roda Bacillus, a koji pokazuju visoku efikasnost u suzbijanju bakteriozne pegavosti paprike.

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Objavljeno
2020/11/16
Broj časopisa
Vol. 24 Br. 2 (2020): Journal on Processing and Energy in Agriculture
Rubrika
Članci